Antigen-specific suppressor cell activity in patients with scleroderma.

Antigen-specific suppressor cell activity (SCA) of peripheral blood mononuclear cells was investigated in 22 patients with scleroderma (PSS) and 22 age and sex matched healthy controls. SCA was induced by priming peripheral blood mononuclear cells with high dose antigen (ovalbumin). Antigen-specific SCA was measured by determining the ability of the primed cells to suppress an optimal ovalbumin specific plaque forming cell (PFC) response in an autologous target culture.

Impaired antigen-specific suppressor cell activity in patients with rheumatoid arthritis.

Antigen-specific suppressor cell activity of peripheral blood mononuclear cells was investigated in 20 patients with rheumatoid arthritis (RA) and 16 age- and sex-matched healthy controls. Suppressor cell activity was generated by priming peripheral blood mononuclear cells with high dose antigen (ovalbumin) and adding the washed primed or control (unprimed) cells to autologous, optimally stimulated, target plaque forming cell (PFC) cultures. The ability of the primed cells to interfere with an optimal ovalbumin specific PFC response in the target culture was used as a measure of antigen-specific suppressor cell activity.

Impaired antigen-specific suppressor cell activity in patients with systemic lupus erythematosus.

Antigen-specific suppressor cell activity of peripheral blood mononuclear cells was investigated in twenty-nine patients with systemic lupus erythematosus (SLE) and sixteen normal, age- and sex-matched healthy controls. Suppressor cell activity was generated by priming peripheral blood mononuclear cells with high dose antigen (ovalbumin) and adding the washed primed or control (unprimed) cells to autologous optimally stimulated target plaque-forming cell (PFC) cultures. The ability of the primed cells to interfere with an optimal ovalbumin-specific PFC response in the target cultures was used as a measure of antigen-specific suppressor cell activity.

Inhibition of mitogen mediated lymphocyte blastogenesis by adenosine.

The effect of adenosine on the proliferative response of human peripheral circulating lymphocytes to stimulation by concanavalin A, phytohemagglutinin and pokeweed mitogen was evaluated. Increasing concentrations of adenosine substantially inhibited mitogen mediated lymphocyte blastogenesis. Erythro-9(2-hydroxyl-3-nonyl) adenine.