Bisphenol A content in fish caught in two different sites of the Tyrrhenian Sea (Italy).

Bisphenol A (BPA) is an endocrine disruptor (ED) that is abundant in the environment because of its extensive use in human-manufactured products. In this study, the BPA concentration was measured in the muscle and liver of five edible fish, characterized by different habitat and habits, caught in two different sites of the Tyrrhenian Sea (Italy). Our results show that: (i) fish livers are about 2.

Apple juice clarification by immobilized pectolytic enzymes in packed or fluidized bed reactors.

The catalytic behavior of a mixture of pectic enzymes, covalently immobilized on different supports (glass microspheres, nylon 6/6 pellets, and PAN beads), was analyzed with a pectin aqueous solution that simulates apple juice. The following parameters were investigated: the rate constant at which pectin hydrolysis is conducted, the time (tau(50)) in which the reduction of 50% of the initial viscosity is reached, and the time (tau(comp,dep)) required to obtain complete depectinization. The best catalytic system was proven to be PAN beads, and their pH and temperature behavior were determined.

Nonisothermal reactors for the production of pure water from peritoneal dialysis waste waters.

The diffusion of peritoneal dialysis (PD) at home is somewhat restricted by the difficulty of transport and storage of a large amount of dialytic solutions. This problem is exacerbated in the case of hemodialysis. With the aim of producing pure water to be used in preparing the solution for peritoneal dialysis, or for hemodialysis in general, as one example, we purified the spent dialysate solution from PD.

Nonisothermal bioreactors in the treatment of vegetation waters from olive oil: laccase versus syringic acid as bioremediation model.

Laccase from Trametes versicolor was immobilized by diazotization on a nylon membrane grafted with glycidil methacrylate, using phenylenediamine as spacer and coupling agent. The behavior of these enzyme derivatives was studied under isothermal and nonisothermal conditions by using syringic acid as substrate, in view of the employment of these membranes in processes of detoxification of vegetation waters from olive oil mills. The pH and temperature dependence of catalytic activity under isothermal conditions has shown that these membranes can be usefully employed under extreme pH and temperatures.

A novel packed-bed bioreactor operating under isothermal and non-isothermal conditions.

A novel packed-bed bioreactor, operating under isothermal and non-isothermal conditions, has been constructed. The core of the apparatus consisted in a polypropylene ring filled with beta-galactosidase immobilized on beads of polyacrylic acid, grafted with dimethylaminoethyl methacrylate. Phenylendiamine and glutaraldehyde were used as spacer and coupling agent, respectively.

In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase.

The influence of an extremely low frequency (ELF) magnetic field (50 Hz and 1 mT, EMF) on the activity of a soluble and insoluble horseradish peroxidase (E.C. 1.

Advantages of using non-isothermal bioreactors in agricultural waste water treatment by means of immobilized urease. Study on the influence of spacer length and immobilization method.

The behavior of three different catalytic membranes, obtained by immobilizing urease on nylon sheets chemically grafted with methyl methacrylate, was studied in a bioreactor operating under isothermal and non-isothermal conditions. Membrane activation was carried out by condensation or acyl azide reaction, and spacers of different lengths, such as hexamethylendiamine or hydrazine, were used. Under isothermal conditions, the activities of the catalytic membranes and soluble urease were characterized as a function of pH, temperature, and urea concentration.

Regional distribution of vasoactive intestinal peptide immunoreactivity in the brain of salmon, trout and carp.

The content of vasoactive intestinal peptide (VIP) was determined by radioimmunoassay in different brain regions of three fish species, namely salmon, trout and carp. The VIP extracted from salmon brain was shown by reverse-phase high-performance liquid chromatography to coelute with native porcine VIP. Highest VIP levels were measured in the salmon brain with a maximum of 112 pg/mg protein in the hypothalamus, followed by the telencephalon, olfactory bulb and optic tectum.

The gill lipids of spawning Pacific salmon.

The gill phospholipids of 2 Pacific salmon species, Oncorhynchus keta and O gorbuscha, were composed of 38-43% phosphatidylcholine (PC) and 19-22% phosphatidylethanolamine (PE). Ether phosphoglycerides constituted 4-8% of the total PC fraction and 37-51% of the PE fraction. No significant changes in gill phospholipids were observed between those of pre-spawning fish in sea water and those of spawned salmon in the river.

Modulation of membrane potential in algal cells by temperature gradients. A thermodynamic approach.

The aim of the present study is to ascertain whether transmembrane temperature gradients couple with transport of electric charge in living cells of Valonia utricularis and eventually measure the thermodynamic coupling coefficient (s). Simple experimental procedures are described that allow generation of temperature gradients of predetermined sense and intensity across the cell membrane. Simultaneous measurement of the potential difference is ensured by standard electrophysiological methods.

Temperature gradients and prebiological evolution.

Thermodiffusive transport of trace elements that play important roles in living organisms, such as molybdenum, nickel, copper, and vanadium, was studied in a nonisothermal biphasic system comprised of a liquid solution and jelly layers. Our intent was to mimic the effects of temperature gradients on prebiological evolution. Conditions were found, similar to those probably existing during development of early eobionts, under which all the elements tested were concentrated within the heated jelly.