[Inhibitory effect of the recombinant adenovirus pre-miR-10a on K562 cell proliferation].

Aim: To investigate the changes of proliferation and apoptosis in K562 cells over-expressing miR-10a.

Methods: K562 cells were infected with pAd-pre-miR-10a virus, and then the levels of miR-10a and bcr/abl in transfected K562 cells was detected using RT-PCR and the level of BCR/ABL using Western blotting. The methylthiazolyl tetrazolium (MTT) assay and flow cytometry (FCM) were used to monitor the changes of proliferation and apoptosis of K562 cells after transfection.