AMPK agonist alleviate renal tubulointerstitial fibrosis via activating mitophagy in high fat and streptozotocin induced diabetic mice.

Renal tubulointerstitial fibrosis was a crucial pathological feature of diabetic nephropathy (DN), and renal tubular injury might associate with abnormal mitophagy. In this study, we investigated the effects and molecular mechanisms of AMPK agonist metformin on mitophagy and cellular injury in renal tubular cell under diabetic condition. The high fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetic mice model and HK-2 cells were used in this study.

Mitochondria-Targeted Peptide SS31 Attenuates Renal Tubulointerstitial Injury via Inhibiting Mitochondrial Fission in Diabetic Mice.

Objective: Renal tubular injury is an early characteristic of diabetic nephropathy (DN) that is related to mitochondrial dysfunction. In this study, we explore the effects and mechanisms of mitochondria-targeted peptide SS31 on renal tubulointerstitial injury in DN.

Method: 40 C57BL/6 mice were randomly divided into control group, STZ group, STZ+SS31 group, and STZ+normal saline group.

HPLC determination and clinical significance of serum prednisone in patients with nephrotic syndrome.

Aim: A rapid protocol is necessary to determine the serum concentrations of prednisone.

Methods: The HP1100 high-performance liquid chromatographic (HPLC) system was employed. The HP Lichrosphere C8 column (250 mm × 4 mm, i.

Influencing factors for saliva urea and its application in chronic kidney disease.

Objectives: To analyze possible influencing factors on concentration of saliva urea (SaU) and to validate its application in chronic kidney disease(CKD).

Design And Methods: Level of SaU in patients and normal subjects was researched.

Results: The concentration of SaU did not vary with sampling time and genders, but was related to ages and level of serum urea (U).