Downregulation of RRM2 Attenuates Retroperitoneal Liposarcoma Progression via the Akt/mTOR/4EBP1 Pathway: Clinical, Biological, and Therapeutic Significance.

Background: Retroperitoneal liposarcoma (RLPS) is a rare tumor with high recurrence rate. Ribonucleotide reductase small subunit M2 (RRM2) protein is essential for DNA synthesis and replication. Our previous study has demonstrated that RRM2 downregulation inhibited the proliferation of RLPS cells, but further association between RRM2 and RLPS and relevant mechanisms remains to be explored.

Inducible de novo expression of neoantigens in tumor cells and mice.

Inducible expression of neoantigens in mice would enable the study of endogenous antigen-specific naïve T cell responses in disease and infection, but has been difficult to generate because leaky antigen expression in the thymus results in central T cell tolerance. Here we develop inversion-induced joined neoantigen (NINJA), using RNA splicing, DNA recombination and three levels of regulation to prevent leakiness and allow tight control over neoantigen expression. We apply NINJA to create tumor cell lines with inducible neoantigen expression, which could be used to study antitumor immunity.

ISLR regulates skeletal muscle atrophy via IGF1-PI3K/Akt-Foxo signaling pathway.

Immunoglobulin superfamily containing leucine-rich repeat (Islr) contains an Ig-like domain, an LRR motif, and a transmembrane domain and is highly expressed in various chicken tissues. Although Islr has known roles in muscle regeneration, its role in the regulation of muscle atrophy has not been studied. In this study, we constructed Islr-silenced or Islr-overexpressed myoblasts to investigate its role during the differentiation of myoblasts into myotubes.

The association between glucose-related variables and plaque morphology in patients with ST-segment elevated myocardial infarction.

Background: Plaque rupture (PR) and plaque erosion (PE) are main causes of acute myocardial infarction with different demographic and histology characteristics and need different treatment strategy. PR and PE can be identified with optical coherence tomography (OCT) accurately, but convenient and effective noninvasive markers for them are rarely found. History of diabetes mellitus (DM) was reported to be a potential predictor of PR in ST-segment elevated myocardial infarction (STEMI) patients, but the predictive value of other glucose-related variables for it is still uncertain.

Identification of TYMS as a promoting factor of retroperitoneal liposarcoma progression: Bioinformatics analysis and biological evidence.

Retroperitoneal liposarcoma (RLPS) is one of the most common types of retroperitoneal sarcomas, and has a high recurrence rate. There is an urgent need to further explore its pathogenesis and develop more effective treatment strategies. The aim of the present study was to identify potential driver genes of RLPS through bioinformatics analysis and molecular biology to elucidate potential targets that are suitable for further analysis for the treatment of RLPS.

Decreased expression of farnesoid X receptor may indicate poor prognosis in patients with colorectal cancer.

Background: Colorectal cancer (CRC) is one of the most common malignancies worldwide. Many studies showed that increased secretion of bile acid may contribute to the pathogenesis of CRC. Farnesoid X receptor (FXR) plays an important role in several physiological functions of bile acid metabolism and circulation, while the diagnostic and prognostic values of FXR remain unknown.

MiR-148a-3p Regulates Skeletal Muscle Satellite Cell Differentiation and Apoptosis via the PI3K/AKT Signaling Pathway by Targeting Meox2.

As bioinformatic approaches have been developed, it has been demonstrated that microRNAs (miRNAs) are involved in the formation of muscles and play important roles in regulation of muscle cell proliferation and differentiation. Previously, it has been demonstrated that miR-148a-3p is one of the most abundant miRNAs in chicken skeletal muscle. Here, we build on that work and demonstrate that miR-148a-3p is important in the control of differentiation of chicken skeletal muscle satellite cells (SMSCs).

Combined exposure to hypoxia and ammonia aggravated biological effects on glucose metabolism, oxidative stress, inflammation and apoptosis in largemouth bass (Micropterus salmoides).

Hypoxia and ammonia are unavoidable environmental factors in aquaculture, and have been shown cause various adverse effects in fish. In the present study, a two-factor crossover experiment was carried out to evaluate the combined effect of hypoxia and ammonia on oxidative stress and glucose metabolism endpoints in largemouth bass. The fish were divided into four experimental groups: hypoxia and ammonia group, hypoxia group, ammonia group, and control group.

Immune-Related Prognostic Model in Colon Cancer: A Gene Expression-Based Study.

Mounting evidence supports that the malignant phenotypes of cancers are defined not only by the intrinsic activity of tumor cells but also by immune cells that are recruited and activated in tumor-related microenvironment. Here, we developed a diagnostic and prognostic model for colon cancer, based on expression profiles of immune-related genes and immune cell component. As a result, we found that B cell infiltration ratio, CD4 T cells, as well as immune-related genes of , , , , , , , and may be highly relevant with clinical outcome of colon cancer.

Quyushengxin Formula Causes Differences in Bacterial and Phage Composition in Ulcerative Colitis Patients.

Background: Ulcerative colitis (UC) is a chronic inflammatory disease that affects the colon and the rectum. Recently, some studies have shown that microorganisms in the gut play important roles in many chronic diseases such as UC.

Methods: To study the candidate viruses and bacteria involved in UC and to investigate the therapeutic mechanism of Quyushengxin formula (QYSX) in UC patients, metagenomic sequencing was performed on the feces from healthy donors and UC patients before and after QYSX treatment.

Prevalence of Prenatal Depression Among Pregnant Women and the Importance of Resilience: A Multi-Site Questionnaire-Based Survey in Mainland China.

Background: Prenatal depression is associated with adverse maternal and fetal health consequences, yet it has not raised sufficient concerns in China. Psychological worries and stress may lead to prenatal depression, but resilience could relieve some of the negative effects of worries and stress and mitigate prenatal depression.

Aims: This study aimed to assess the prevalence of prenatal depression and explore its associated factors.

Measurement of the adjustable slight roughness emulated by a spatial light modulator employing the vortex beam speckle pattern.

In this paper, we analyze the speckle patterns generated by the scattering of optical vortex on different roughness surfaces, compared with those produced by Gaussian light, where the roughness is emulated by a spatial light modulator and adjustable. The scheme and experiments demonstrate an improvement with the vortex beam, especially for slight roughness measurements. In addition, since the topological charge used to produce speckle patterns has a great influence on the speckle size and can be optimized to adjust it for different object measurements, we also investigate how the roughness depends on the topological charge.

In Situ Formed LiZn Alloy Skeleton for Stable Lithium Anodes.

Lithium metal is the most promising anode for developing high-energy density rechargeable batteries because of its ultrahigh theoretical capacity and extremely low reduction potential. However, the formation of dendritic lithium and the huge volume change of the anode during the charge/discharge process severely hinder the practical application of the lithium anode. Obtaining high-performance, simple methods that can simultaneously modify the interface and restrict the volume change of the Li anode are highly required.

A novel prognostic model based on multi-omics features predicts the prognosis of colon cancer patients.

Background: As a common malignant tumor in the colon, colon cancer (CC) has high incidence and recurrence rates. This study is designed to build a prognostic model for CC.

Methods: The gene expression dataset, microRNA-seq dataset, copy number variation (CNV) dataset, DNA methylation dataset, and transcription factor (TF) dataset of CC were downloaded from UCSC Xena database.

MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF‑β2/SMAD Signaling Pathway.

MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19.

Interface Defects Passivation and Conductivity Improvement in Planar Perovskite Solar Cells Using NaS-Doped Compact TiO Electron Transport Layers.

Numerous trap states and low conductivity of compact TiO layers are major obstacles for achieving high power conversion efficiency and high-stability perovskite solar cells. Here we report an effective NaS-doped TiO layer, which can improve the conductivity of TiO layers, the contact of the TiO/perovskite interface, and the crystallinity of perovskite layers. Comprehensive investigations demonstrate that Na cations increase the conductivity of TiO layers while S anions change the wettability of TiO layers, thus improving the crystallinity of perovskite layers and passivate defects at the TiO/PVK interface.

Enhancing ZnO nanowire gas sensors using Au/FeO hybrid nanoparticle decoration.

Heterojunctions are an important strategy for designing high performance electrical sensor materials and related devices. Herein, a new type of metal-semiconductor hybrid nanoparticle has been successfully used to remarkably sensitize the surface of ZnO nanowires for detecting NO with high responses over a broad temperature window ranging from room temperature to 600 °C. These hybrid nanoparticles are comprised of iron oxide nanowires with well dispersed single crystalline Au nanoparticles.

Maximized lateral inhibition in paired magnetic domain wall racetracks for neuromorphic computing.

Lateral inhibition is an important functionality in neuromorphic computing, modeled after the biological neuron behavior that a firing neuron deactivates its neighbors belonging to the same layer and prevents them from firing. In most neuromorphic hardware platforms lateral inhibition is implemented by external circuitry, thereby decreasing the energy efficiency and increasing the area overhead of such systems. Recently, the domain wall-magnetic tunnel junction (DW-MTJ) artificial neuron is demonstrated in modeling to be intrinsically inhibitory.

Molecular characterization, tissue distribution, and functional analysis of the gene in chicken.

The Src homology 3 and cysteine-rich domain 3 gene () encodes a protein containing both a cysteine-rich domain and two Src (sarcoma) homology 3 domains (SH3). is specifically expressed in skeletal muscle and plays an important role in skeletal muscle development, but the explicit sequence and function of chicken remain unknown. In the current study, we found the full-length chicken cDNA to be 1383 bp long, with a 1092 bp open reading frame that harbors one cysteine-rich C1 domain and two SH3 domains.

What Drives the Performance of Collaboration Networks: A Qualitative Comparative Analysis of Local Water Governance in China.

Local water governance is challenging given the significance to public health and the difficulties to manage it in a fragmented administrative system. A collaboration network is a popular governance tool in local governance to cope with functional fragmentation problems and institutional collective action (ICA) dilemmas. Empirical works are needed to examine the outcomes of such governance networks, especially in the context of environmental governance.

Effects of vacancy defects on the mechanical properties in neutron irradiated Czochralski silicon.

We have investigated the mechanical properties of neutron irradiated Czochralski (NICZ) silicon using nanoindentations combined with micro-Raman spectroscopy. It is found that NICZ silicon shows higher hardness (∼13% higher) than non-irradiated silicon, with a slightly lower Young's modulus. When the samples were subjected to isochronal anneals in the temperature range of 250 °C-650 °C, the hardness of NICZ silicon gradually decreases as the temperature increases and it is finally comparable to that of the non-irradiated silicon.

Exosomes Transmit Viral Genetic Information and Immune Signals may cause Immunosuppression and Immune Tolerance in ALV-J Infected HD11 cells.

Avian leukosis virus (ALV) is oncogenic retrovirus that not only causes immunosuppression but also enhances the host's susceptibility to secondary infection. Exosomes play vital role in the signal transduction cascades that occur in response to viral infection. We want to explore the function of exosomes in the spread of ALV and the body's subsequent immunological response.

Association between ZNF184 and symptoms of Parkinson's disease in southern Chinese.

Study Objectives: The aim was to investigate the association between ZNF184 and symptoms of Parkinson's disease (PD) in southern Chinese.

Methods: A total of 241 PD patients were recruited in this study. All patients were evaluated by Sniffin' Sticks 16 (SS-16), Hamilton anxiety rating scale and Hamilton depression rating scale, 39-item Parkinson's disease Questionnaire (PDQ-39) and MDS-Unified PD Rating Scale (MDS-UPDRS).

Understanding the Sociocognitive Process of Construction Workers' Unsafe Behaviors: An Agent-Based Modeling Approach.

Previous literature has recognized that workers' unsafe behavior is the combined result of both isolated individual cognitive processes and their interaction with others. Based on the consideration of both individual cognitive factors and social organizational factors, this paper aims to develop an Agent-Based Modeling (ABM) approach to explore construction workers' sociocognitive processes under the interaction with managers, coworkers, and foremen. The developed model is applied to explore the causes of cognitive failure of construction workers and the influence of social groups and social organizational factors on the workers' unsafe behavior.