Publications by authors named "Camino Guerra"

Germplasm banking is a key technology enabling the ex-situ conservation of wild species. However, cryopreservation protocols must be tested to assure the applicability of the banked material. The objective of this study was defining a range of parameters for the composition of a semen extender for Cantabrian chamois epididymal spermatozoa (post-mortem collection).

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We tested the protective action of seminal plasma on epididymal spermatozoa from Iberian red deer, especially considering cryopreservation, as a means for germplasm banking improvement. We obtained seminal plasma by centrifuging electroejaculated semen, and part of it was thermically inactivated (denatured plasma; 55 degrees C 30 min). Epididymal samples (always at 5 degrees C) were obtained from genitalia harvested after regulated hunting, and pooled for each assay (five in total).

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Article Synopsis
  • Seasonality plays a crucial role in the physiology and reproductive behaviors of Iberian red deer, roe deer, and Cantabrian chamois, indicating a need for careful timing in biological resource bank (BRB) establishment.
  • The study involved collecting and analyzing testicles from hunted deer at various times of the year, focusing on multiple reproductive parameters and sperm quality across defined seasonal periods.
  • Results show that while the rutting/breeding season generally yields higher reproductive metrics, specific species display variations in sperm quality, suggesting optimal times for sperm collection vary by species and season.
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