Application of Doehlert design combined with chemometrics tools: Example of the optimization of the elution of neurotransmitters and metabolites by HPLC.

Experimental designs are essential mathematical tools in fields like agronomy, chemistry, and analytical chemistry for optimizing processes and minimizing variations. Doehlert designs, in particular, are valued for their efficiency in exploring experimental space with minimal experiments, providing detailed insights into complex processes. In analytical chemistry, these designs are extensively used for tasks such as extraction, purification, and method optimization, allowing systematic variation of factors to enhance accuracy and efficiency.