Publications by authors named "Camille Lasserre"
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated three MALDI-TOF-based techniques to detect carbapenemase-producing Enterobacteriaceae (CPE) from cultured colonies.
Methods: The performance of three MALDI-TOF-based techniques, including the commercialized MBT STAR®-Carba IVD Kit (Bruker Daltonics) and two in-house protocols performed on the Microflex LT Biotyper (Bruker Daltonics) and the VITEK® MS Plus (bioMérieux), were compared with those of the RAPIDEC® CARBA NP (bioMérieux).
Article Synopsis
- Carbapenemase-producing Enterobacteriaceae (CPE) detection is crucial for effective patient management and infection control, but current methods can be challenging and slow.
- The study introduces a rapid and cost-effective matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) method that involves incubating bacterial isolates with imipenem and analyzing the supernatant for specific metabolites.
- Validation of this method showed excellent repeatability and 100% accuracy in identifying CPE strains, making it a reliable option for routine laboratory use, costing less than 10 cents per test.
Objective: Our goal was to estimate the performance statistics of an electronic surveillance system for surgical site infections (SSIs), generally applicable in French hospitals.
Methods: Three detection algorithms using 2 different data sources were tested retrospectively on 9 types of surgical procedures performed between January 2010 and December 2011 in the University Hospital of Nantes. The first algorithm was based on administrative codes, the second was based on bacteriological data, and the third used both data sources.