Genotyping of SNPs in bread wheat at reduced cost from pooled experiments and imputation.

Pooling and imputation are computational methods that can be combined for achieving cost-effective and accurate high-density genotyping of both common and rare variants, as demonstrated in a MAGIC wheat population. The plant breeding industry has shown growing interest in using the genotype data of relevant markers for performing selection of new competitive varieties. The selection usually benefits from large amounts of marker data, and it is therefore crucial to dispose of data collection methods that are both cost-effective and reliable.

A joint use of pooling and imputation for genotyping SNPs.

Background: Despite continuing technological advances, the cost for large-scale genotyping of a high number of samples can be prohibitive. The purpose of this study is to design a cost-saving strategy for SNP genotyping. We suggest making use of pooling, a group testing technique, to drop the amount of SNP arrays needed.