Canine IVM With SOF Medium, Insulin-Transferrin-Selenium, and Low O Tension Improves Oocyte Meiotic Competence and Decreases Reactive Oxygen Species Levels.

Assisted reproductive technologies in canine species are limited due to the low efficiency of maturation (IVM). Unlike other mammals, bitches ovulate oocytes in the germinal vesicle stage and complete metaphase II (MII) after 48-72 h in the oviductal environment and become fertilizable. For this reason, we compared two different IVM media, synthetic oviductal fluid (SOF) supplemented with 8% bovine serum albumin (BSA) or a mixture of 8% BSA-2.