Angiogenesis and osteogenesis at incorporation process of onlay bone graft.

Purpose: The roles of angiogenesis and osteogenesis in autologous and allogenic bone grafts and the use of platelet-rich plasma (PRP) as a modifier were investigated.

Materials And Methods: Forty rabbit mandibles received onlay grafts of fresh autologous and frozen allogeneic bone. PRP was added on the right side.

Pattern of osteogenesis during onlay bone graft healing.

Objective: The aim of this paper was to analyze how healing occurs between onlay bone graft and the mandible cortex.

Study Design: Autologous and allogeneic corticocancellous bones, harvested from the ilium wing, were grafted at each mandible side of 40 rabbits. One side received platelet-rich plasma (PRP).

Effects of freezing on bone histological morphology.

Allograft bone has been widely used for reconstruction of different portions of the skeleton. The fragment of bone harvested must be kept under low temperatures. The cryopreservation also contributes to decrease the antigenic potential of the tissue.

Evaluation of factors that can modify platelet-rich plasma properties.

Objective: The aim of this article is to discuss a protocol for obtaining platelet-rich plasma (PRP) and evaluate which factors, derived from its preparation method and from whole blood, modify PRP cytometry and coagulation time.

Study Design: Whole blood, harvested from 50 rabbits, was centrifuged at 300g for 10 minutes. Supernatant was recentrifuged at 5000g for 5 minutes.