Specific identification of by Loop-Mediated Isothermal Amplification (LAMP) targeting the Region of Difference 12 (RD12) of the complex.

Bovine tuberculosis is a prevalent zoonotic disease that causes high risks for production animals, dairy producers and consumers, together with significant economic losses. Thus, methods for easy, fast and specific detection of in small and medium-sized livestock under field conditions are very required. In this work, a Loop-Mediated Isothermal Amplification LAMP-PCR targeting the Region of Difference 12 (RD12) of genome was designed for the purpose of identification.