Publications by authors named "Camila Guariz Homem"
Cryptosporidiosis is an emerging zoonotic disease caused by the worldwide distributed parasitic protozoa Cryptosporidium spp. The host-adapted species Cryptosporidium canis is most frequently found in dogs, although human infections with this species have been described. This study aimed to develop a real-time PCR targeting the HSP70 protein gene for C.
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- The study focuses on detecting Cryptosporidium serpentis, a parasite causing gastritis in snakes, using real-time PCR, which targets the Hsp70 gene.
- Fecal samples from 503 asymptomatic adult snakes were analyzed, revealing 60 positive cases of Cryptosporidium sp. using nested PCR, with specific species identified through sequencing.
- Real-time PCR showed a 93.8% sensitivity and 99.5% specificity for detecting C. serpentis, confirming its effectiveness compared to the traditional nested PCR method.