Publications by authors named "Cameron L Gardner"
Recombination-activating genes (RAG1 and RAG2) are critical for lymphoid cell development and function by initiating the variable (V), diversity (D), and joining (J) (V(D)J)-recombination process to generate polyclonal lymphocytes with broad antigen specificity. The clinical manifestations of defective RAG1/2 genes range from immune dysregulation to severe combined immunodeficiencies (SCIDs), causing life-threatening infections and death early in life without hematopoietic cell transplantation (HCT). Despite improvements, haploidentical HCT without myeloablative conditioning carries a high risk of graft failure and incomplete immune reconstitution.
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- Severe combined immune deficiency (SCID) due to RAG1 or RAG2 deficiency results in a lack of T and B lymphocytes, leading to high vulnerability to severe infections without treatment.
- Hematopoietic stem cell transplantation (HSCT) is the primary treatment but has risks like graft-versus-host disease, graft failure, and poor immune recovery.
- Recent research using gene editing on patient-derived iPSCs shows promise in restoring T cell development in RAG2-deficient cells, suggesting targeted gene therapy could be a new treatment approach for this condition.
Article Synopsis
- * Results showed that AK2 deficiency and a null IL2RG mutation led to early T-cell development blocks, while a missense IL2RG mutation allowed for some maturation despite lower cell numbers.
- * The ATO system proves useful in distinguishing between hematopoietic and thymic defects in T-cell deficiency and identifying specific stages where T-cell differentiation is hindered.
Recoding--the repurposing of genetic codons--is a powerful strategy for enhancing genomes with functions not commonly found in nature. Here, we report computational design, synthesis, and progress toward assembly of a 3.97-megabase, 57-codon Escherichia coli genome in which all 62,214 instances of seven codons were replaced with synonymous alternatives across all protein-coding genes.
View Article and Find Full Text PDFA microbial fuel cell (MFC) is a bio-inspired renewable energy converter which directly converts biomass into electricity. This is accomplished via the unique extracellular electron transfer (EET) of a specific species of microbe called the exoelectrogen. Many studies have attempted to improve the power density of MFCs, yet the reported power density is still nearly two orders of magnitude lower than other power sources/converters.
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