Transforming urban planning processes and outcomes through creative methods.

Inclusively delivering the sustainable development goals (SDGs) remains challenging, particularly in urban areas, where some of the most pressing concerns exist. To achieve the transformative SDG agenda, new methods are required to overcome current deficits in engagement around inclusion and equitable outcomes. Evaluating against theories of governance and inclusion, we test a mixture of digital and physical creative methods abilities to deliver co-designed solutions that influence mobility and road safety planning outcomes in East African cities.

Co-designing Urban Living Solutions to Improve Older People's Mobility and Well-Being.

Mobility is a key aspect of active ageing enabling participation and autonomy into later life. Remaining active brings multiple physical but also social benefits leading to higher levels of well-being. With globally increasing levels of urbanisation alongside demographic shifts meaning in many parts of the world this urban population will be older people, the challenge is how cities should evolve to enable so-called active ageing.

Co-Motion: Mobility and Wellbeing in Later Life.

In considering the role of place in supporting positive well-being choices for all, including older people, there has been an almost exclusive focus on issues of design in the public realm. Emerging findings from the Co-Motion project suggest that the experience of being out and about can be also facilitated or profoundly damaged by the attitudes and behaviours of fellow public realm users.

Heat stress: a major contributor to poor animal welfare associated with long-haul live export voyages.

Recent investigations by the Australian Department of Agriculture, Fisheries and Forestry into high mortalities on live export voyages from Australia to the Middle East during the Northern hemisphere summer suggest that animal welfare may be compromised by heat stress. The live export industry has generated a computer model that aims to assess the risk of heat stress and to contain mortality levels on live export ships below certain arbitrary limits. Although the model must be complied with under Australian law, it is not currently available for independent scientific scrutiny, and there is concern that model and the mandated space allowances are inadequate.

The questionable value of some science-based 'welfare' assessments in intensive animal farming: sow stalls as an illustrative example.

A recent review of the code of practice for pigs brought attention to the question of how to assess the impact of housing conditions on pig welfare. The stance adopted by the law-makers, which mirrors that of industry, is that the status quo should be maintained until there is irrefutable scientific evidence in favour of change. Sows in intensive pig farms are often confined in cages (sow stalls) that are little bigger than their body.

Comparison of different stomatal conductance algorithms for ozone flux modelling.

A multiplicative and a semi-mechanistic, BWB-type [Ball, J.T., Woodrow, I.

Modelling stomatal ozone flux across Europe.

A model has been developed to estimate stomatal ozone flux across Europe for a number of important species. An initial application of this model is illustrated for two species, wheat and beech. The model calculates ozone flux using European Monitoring and Evaluation Programme (EMEP) model ozone concentrations in combination with estimates of the atmospheric, boundary layer and stomatal resistances to ozone transfer.

Effects of anti-arthritic drugs on proteoglycan synthesis by equine cartilage.

The concentration-effect relationships of phenylbutazone, indomethacin, betamethasone, pentosan polysulphate (PPS) and polysulphated glycosaminoglycan (PSGAG), on proteoglycan synthesis by equine cultured chondrocytes grown in monolayers, and articular cartilage explants were measured. The effect of PSGAG on interleukin-1beta induced suppression of proteogycan synthesis was also investigated. Proteoglycan synthesis was measured by scintillation assay of radiolabelled sulphate (35SO4) incorporation.

Kinin B2 and B1 receptor-mediated vasoactive effects in rabbit synovium.

Blood flow in response to bradykinin (BK, B2 receptor agonist) and desArg9 BK (B1 receptor agonist) was measured by laser Doppler flowmetry, as a reversal of noradrenaline (50 nmol)-induced decreased blood flow, in the synovium of the anaesthetised rabbit. Either a pretreatment (-6 h) of the cytokines IL-1beta (10 pmol) plus TNFalpha (10 pmol) or saline was injected intra-articularly. BK increased blood flow irrespective of pretreatment, whereas desArg9BK increased blood flow only in the cytokine-pretreated joints.

Calcitonin gene-related peptide: vasoactive effects and potential therapeutic role.

1. The cardiovascular biology of calcitonin gene-related peptide (CGRP) and the structurally related peptides amylin and adrenomedullin are briefly reviewed. 2.

Investigation of 6-hydroxydopamine-induced plasma extravasation in rat skin.

Perfusion of 6-hydroxydopamine into the rat knee and trachea induces plasma extravasation, possibly by tissue-specific mechanisms involving sympathetic and sensory nerves respectively, and we aimed to identify the mediators which contribute to this response in skin. 6-Hydroxydopamine (both hydrobromide and hydrochloride salts), dose dependently increased plasma extravasation into rat dorsal skin, however, when compared to bradykinin or the tachykinin NK1 receptor agonist GR73632, high concentrations of 6-hydroxydopamine (1-10 mumol/site) were required. The response to 6-hydroxydopamine was not inhibited in chemically sympathectomised rats (6-hydroxydopamine, 300 mg/kg i.

The effect of intra-articular capsaicin on nerve fibres within the synovium of the rat knee joint.

The aim of this study was to establish the effects of intra-articular capsaicin (pelargonic acid vallinylamide) on synovial innervation of the rat knee. Rats were sacrificed 1, 2, 4 and 7 days after intra-articular injection of capsaicin and joint tissues stained with either conventional haematoxylin and eosin (H and E) or with specific antibodies to the calcitonin gene-related peptide (CGRP), substance P (both of which are markers for primary afferent fibres), the C-flanking peptide of neuropeptide Y (CPON) (localised in postganglionic sympathetic fibres), or protein gene product 9.5 (a pan-neuronal marker).

Mechanism of bradykinin-induced plasma extravasation in the rat knee joint.

1. We have investigated the mechanism of bradykinin (BK)-induced plasma extravasation into the knee joint of the anaesthetized rat. Accumulation of [125I]-human serum albumin within the synovial cavity was used as a marker of increased vascular permeability.

The effect of intra-articular capsaicin on passive synovial anaphylaxis and blood flow in the rat knee joint.

Normal rat and human synovium is innervated by small diameter, unmyelinated, peptide-containing nerves. A close anatomical association between these nerves and mast cells has been postulated23, although functional interactions have not been described. Capsaicin is frequently used to activate sensory nerves and we have examined both acute and long-term effects of capsaicin on passive synovial anaphylaxis (PSA) and blood flow in the rat knee joint.

The contribution of calcitonin gene-related peptide (CGRP) to neurogenic vasodilator responses.

The neuropeptide calcitonin gene-related peptide (CGRP) is a potent vasodilator in the microcirculation of many tissues including the skin and joint. In order to elucidate the mechanism of endogenous CGRP release, we have used a multiple site 133Xe clearance technique to measure local blood flow changes in response to agents injected intradermally in the rabbit. Capsaicin (100 nmol/site) and human alpha CGRP (3 pmol/site) stimulated similar increases in blood flow and, in both cases, the effect was totally abolished by the CGRP antagonist, CGRP8-37 (1 nmol/site).

Calcitonin gene-related peptide increases blood flow and potentiates plasma protein extravasation in the rat knee joint.

1. The effects of calcitonin gene-related peptide (CGRP) and other vasoactive mediators of inflammation on blood flow in the synovial vessels and plasma protein extravasation into the knee (femoro-tibial) joint of the pentobarbitone-anaesthetized rat were measured. 2.

Evidence that calcitonin gene-related peptide contributes to inflammation in the skin and joint.

Calcitonin gene-related peptide produces dose-related vasodilatation after intradermal injection in several species. In the present study, CGRP increased blood flow in rabbit skin but had no direct effect on edema formation in rat or rabbit skin or in the rat knee joint. However, CGRP produced significant potentiation of edema formation when co-injected with histamine, a potent mediator of increased vascular permeability.

Influence of feeding schedule on the absorption of orally administered flunixin in the horse.

The effects of access to hay and of restricted feeding on the pharmacokinetics of flunixin administered orally to six healthy ponies were compared in a cross-over study. No access to feed for a few hours before and after flunixin administration resulted in rapid absorption with a mean peak plasma concentration of 2.84 +/- 0.

Antithrombotic actions of aspirin in the horse.

The antithrombotic effects of aspirin at two dose rates (4 mg/kg and 11 mg/kg bodyweight [bwt] were evaluated in normal, healthy ponies by measuring template bleeding time. Inhibition of platelet aggregation in response to adenosine diphosphate (ADP) and collagen was evaluated and cyclo-oxygenase activity was monitored by radioimmunoassay of thromboxane B2 (TXB2), the stable metabolite of thromboxane A2 (TXA2). TXB2 was measured in serum and platelet rich plasma.

Functional and morphological studies on blood platelets in a thrombasthenic horse.

A four-year-old Standardbred gelding presented with a 3.5 year history of intermittent epistaxis and spontaneous submucosal petechiae and ecchymoses in the nares and the mouth. Routine haematological and biochemical examinations were unremarkable.

The effect of Strongylus vulgaris larvae on equine intestinal myoelectrical activity.

The myoelectrical activity of the ileum, caecum and large colon was monitored from Ag-AgCl bipolar recording electrodes in four conscious 'parasite-naive' weanling foals. All foals were inoculated with 1000 infective 3rd-stage Strongylus vulgaris larvae and alterations to the myoelectrical activity observed. The frequencies of caecal and colonic spike bursts increased significantly in all post infection periods coinciding with assumed larval penetration into the intestinal mucosa and migration through the vasculature.

Plasma thromboxane B2 levels in horses experimentally infected with Strongylus vulgaris.

Plasma thromboxane B2 (TXB2) the stable inactive metabolite of thromboxane A2 (TXA2), was measured daily by specific radioimmunoassay in three groups of animals before and after experimental infection with Strongylus vulgaris. Infection of four 'parasite naive' foals produced a typical acute syndrome with intermittent but statistically insignificant rises in TXB2 levels. Interpretation of results was complicated by the presence of a non-septic peritonitis associated with implantation of the foals with electrodes for recording myoelectrical activity.

Radioimmunoassay of thromboxane B2 in horse plasma.

A radioimmunoassay for thromboxane B2 (TXB2) in unextracted horse plasma was evaluated. Sensitivity of the assay was 14.0 (SD 5.