Correlating Disordered Activation Domain Ensembles with Gene Expression Levels.

Transcription factor proteins bind to specific DNA promoter sequences and initiate gene transcription. These proteins often contain intrinsically disordered activation domains (ADs) that regulate their transcriptional activity. Like other disordered protein regions, ADs do not have a fixed three-dimensional structure and instead exist in an ensemble of conformations.

Exploring the potential of cryo-FESEM for the taxonomic study of aquatic microcrustaceans: Bathynellacea (Crustacea, Malacostraca) as an example.

The taxonomic study of microcrustaceans is a time consuming and challenging endeavor, which has slowed the rate of new discoveries, and in turn knowledge on, global aquatic biodiversity. To facilitate the study of these small organisms, new applications continually need to be explored. Here, we assess the potential use of environmental scanning electron microscopy (ESEM) techniques, specifically cryo-field emission SEM (cryo-FESEM), for taxonomic descriptions of microcrustaceans.

Differential diagnosis and comparison of diagnostic algorithms in children and adolescents with autoimmune encephalitis in Spain: a prospective cohort study and retrospective analysis.

Background: The usefulness of current diagnostic approaches in children with suspected autoimmune encephalitis is unknown. We aimed to assess the diagnosis of autoimmune encephalitis in clinical practice and to compare the performance of two international diagnostic algorithms (one intended for patients of any age [general], the other intended for paediatric patients), with particular emphasis on the evaluation of patients with probable antibody-negative autoimmune encephalitis because this diagnosis suggests that immunotherapy should be continued or escalated but is difficult to establish.

Methods: We did a prospective cohort study that included all patients (<18 years of age) with suspected autoimmune encephalitis recruited at 40 hospitals in Spain whose physicians provided clinical information every 6 months for 2 years or more.

Unilateral Duplication of the Internal Auditory Canal: Stenosis and Incomplete Transverse Megacrest in a Pediatric Patient.

Based on the available literature, duplicated internal auditory canals (DIACs) represent an exceedingly rare temporal bone anomaly that can result in sensorineural hearing loss (SNHL) in the pediatric population. Often associated with a hypoplastic or aplastic cochlear nerve, DIAC poses limitations on treatment options, such as cochlear implants, for affected patients. Accurate diagnosis and optimal management necessitate a thorough assessment of inner ear structures and potential neural abnormalities with high-resolution computed tomography and magnetic resonance imaging of the temporal bones.

Conjunctival Neuroma After Corneal Neurotization in a Patient With Neurotrophic Keratopathy: A Case Report.

Purpose: To report a rare case of conjunctival neuroma after a successful corneal neurotization surgery.

Methods: The clinical file and histopathology slides of this patient who underwent surgical corneal neurotization for a neurotrophic keratopathy in the right eye were reviewed.

Results: A 70-year-old man with a history of severe herpetic (varicella zoster) neurotrophic keratopathy and keratouveitis in the right eye developed a corneal perforation, which required tectonic keratoplasty (May 2020).

A new species of Neblinaphryne (Anura: Brachycephaloidea: Neblinaphrynidae) from Serra do Imeri, Amazonas state, Brazil.

The highlands of the Guiana Shield (Pantepui) in northern South America harbor a unique fauna and flora. However, this diversity remains poorly documented, as many Pantepui massifs remain little explored or unexplored, mainly because their access is very challenging. Considering amphibians, 11 genera are endemic or sub-endemic to Pantepui, and one of them, Neblinaphryne, is monospecific and was recently described from the Neblina massif, at the border between Brazil and Venezuela.

Emerging Therapies for Brain Metastases in NSCLC, Breast Cancer, and Melanoma: A Critical Review.

Purpose Of Review: Advancements in precision medicine have shifted the treatment paradigm of brain metastases (BM) from non-small cell lung cancer (NSCLC), breast cancer, and melanoma, especially through targeted therapies focused on specific molecular drivers. These novel agents have improved outcomes by overcoming challenges posed by the blood-brain barrier (BBB) and resistance mechanisms, enabling more effective treatment of BM.

Recent Findings: In NSCLC, therapies such as osimertinib have improved efficacy in treating EGFR-mutant BM, with emerging combinations such as amivantamab and lazertinib offering promising alternatives for patients resistant to frontline therapies.

Trophic status and metabolic rates of threatened shallow saline lakes in Central Spain: Providing diagnostic elements for improving management strategies.

Shallow saline lakes in the La Mancha Húmeda Biosphere Reserve in Central Spain show diverse degrees of cultural and natural eutrophication, prompting urgent conservation measures. This study focuses on 17 representative lakes from the site to assess seasonal nutrient dynamics and their connection to plankton metabolism (photosynthesis and respiration) during two successive hydrological periods. Effect of environmental factors was evaluated on a combination of several response variables, demonstrating that source of the nutrient inputs (ranging from natural to anthropic) had the highest influence on the nutrients stoichiometry and metabolic rates.

Strain Partitioning in a Flattening Shear Zone: Re-Evaluation of a Cycladic Style Detachment.

The Almyropotamos tectonic window on southern Evia island in the NW Aegean Sea divides two high pressure-low temperature metamorphic units, representing distinct Hellenic thrust sheets. Ductile thinning along the major low-angle Evia Shear Zone has closely juxtaposed the lower (Basal Unit) marble-flysch sequence structurally below Styra marbles (Cycladic Blueschist Unit). The partially attenuated flysch comprises a matrix dominated by pelitic schist, with dispersed cm- to hm-scale blocks of marble, carbonate schist, quartzite, and metabasite.

Ecological status and type of alteration determine the C-balance and climate change mitigation capacity of Mediterranean inland saline shallow lakes.

Saline shallow lakes may play an important role in carbon exchange with the atmosphere, but their alteration may change carbon balance and greenhouse gas emissions patterns. This study investigated the relationship between carbon metabolic processes and environmental factors, focusing on alterations in salinity, hydroperiod length, and trophic status. The results revealed that disruptions to natural salinity patterns, driven by hydrological changes and ecological degradation, enhanced carbon greenhouse gas emitting metabolisms.

Experimental Assessment of Corrosion Properties for Materials Intended for Heavy Crude Processing.

Heavy crude oil processing presents significant challenges owing to its complex composition and requirement for processing conditions, which increase the process safety risk in crude processing units, such as fixed equipment, for instance pressure vessels and pipes. The aim of this work is to evaluate the influence of heavy crude oils named A and B and the effect of sulfur-rich compounds and organic acids on the performance at high temperatures of three metallic alloys (5Cr-1/2Mo/ASTM A335GP5, X6CrNiMoTi17122/AISI-SAE 316Ti and Ni66.5Cu31.

Use of Telenursing in the conservative treatment of patients with chronic renal insufficiency: scoping review.

Objective: to map the evidence of the use of telenursing in adherence to treatment and promotion of self-care in patients with chronic renal insufficiency undergoing conservative treatment.

Method: scoping review study, with selection of documents indexed in eight databases and two catalogs of theses and dissertations. Additionally, the reference lists of selected studies were consulted.

Bacterial Binding to Polydopamine-Coated Magnetic Nanoparticles.

In medical infections such as blood sepsis and in food quality control, fast and accurate bacteria analysis is required. Using magnetic nanoparticles (MNPs) for bacterial capture and concentration is very promising for rapid analysis. When MNPs are functionalized with the proper surface chemistry, they have the ability to bind to bacteria and aid in the removal and concentration of bacteria from a sample for further analysis.

Ensembling U-Nets for microaneurysm segmentation in optical coherence tomography angiography in patients with diabetic retinopathy.

Diabetic retinopathy is one of the leading causes of blindness around the world. This makes early diagnosis and treatment important in preventing vision loss in a large number of patients. Microaneurysms are the key hallmark of the early stage of the disease, non-proliferative diabetic retinopathy, and can be detected using OCT angiography quickly and non-invasively.

Environmental factors affecting phenology and distribution of Tentyria species (Coleoptera: Tenebrionidae) in Doñana National Park (Southern Iberian Peninsula).

This research focuses on the effect of environmental factors on the phenology and distribution of the Tentyria species (Coleoptera: Tenebrionidae) from Doñana National Park (SW Iberian Peninsula). Data are derived from the results of a project carried out 20 years ago, aimed at inventorying the coleopteran of the park. This information provides a framework for comparison with current or future states since the time elapsed is long enough to detect variations.

Immunogenicity of an Extended Dose Interval for the Ad26.ZEBOV, MVA-BN-Filo Ebola Vaccine Regimen in Adults and Children in the Democratic Republic of the Congo.

During the 2018-2020 Ebola virus disease outbreak in Democratic Republic of the Congo, a phase 3 trial of the Ad26.ZEBOV, MVA-BN-Filo Ebola vaccine (DRC-EB-001) commenced in Goma, with participants being offered the two-dose regimen given 56 days apart. Suspension of trial activities in 2020 due to the COVID-19 pandemic led to some participants receiving a late dose 2 outside the planned interval.

Serological and molecular survey of rat hepatitis E virus () in drug users.

Rat hepatitis E virus (ratHEV) is an emerging cause of acute hepatitis of zoonotic origin. Since seroprevalence studies are scarce, at-risk groups are almost unknown. Because blood-borne infections frequently occur in people with drug use, who are particularly vulnerable to infection due to lack of housing and homelessness, this population constitutes a priority in which ratHEV infection should be evaluated.

Effectiveness of rVSV-ZEBOV vaccination during the 2018-20 Ebola virus disease epidemic in the Democratic Republic of the Congo: a retrospective test-negative study.

Background: The recombinant vesicular stomatitis virus-Zaire Ebola virus (rVSV-ZEBOV) vaccine is the only WHO prequalified vaccine recommended for use to respond to outbreaks of Ebola virus (species Zaire ebolavirus) by WHO's Strategic Advisory Group of Experts on Immunization. Despite the vaccine's widespread use during several outbreaks, no real-world effectiveness estimates are currently available in the literature.

Methods: We conducted a retrospective test-negative analysis to estimate effectiveness of rVSV-ZEBOV vaccination against Ebola virus disease during the 2018-20 epidemic in the Democratic Republic of the Congo, using data on suspected Ebola virus disease cases collected from Ebola treatment centres.

The first report of Bathynellacea in the subterranean water of Mongolia: A new species of Camacho, 2020 (Crustacea, Bathynellidae).

Background: We report the first finding of Bathynellacea, discovered in Mongolia. We also report a new species of the genus Camacho, 2020, which was previously recorded only in the western edge of Russia.

New Information: sp.

Cost-effective fully 3D-printed on-drop electrochemical sensor based on carbon black/polylactic acid: a comparative study with screen-printed sensors in food analysis.

3D-printing technology allows scientist to fabricate easily electrochemical sensors. Until now, these sensors were designed employing a large amount of material, which increases the cost and decreases manufacturing throughput. In this work, a low-cost 3D-printed on-drop electrochemical sensor (3D-PES) was fully manufactured by fused filament fabrication, minimizing the number of printing layers.

Bilateral stringhalt associated with Hypochaeris radicata in Uruguayan horses.

We reported thirteen cases of bilateral stringhalt associated with Hypochaeris radicata that occurred in horses in Uruguay during a severe drought in the summer of 2023. All horses were affected chronically and progressively by bilateral hyperflexion of hindlimbs. In two severely affected horses, the main histological lesions included neuronal chromatolysis and axonal spheroids in the ventral gray horn in the lumbar and sacral spinal cord and axonal degeneration and digestion chambers in ventral roots fibers and long peripheral nerves.