Electrostatic-driven self-sorting and nanostructure speciation in self-assembling tetrapeptides.

Significant efforts in the field of supramolecular materials have strived to co-assemble small molecules in order to realize individual nanostructures with multiple, tunable activities. The design of self-assembling motifs bearing opposite charges is one commonly used method, with favorable electrostatic interactions used to promote mixing in a resulting co-assembly. This approach, at the same time, contrasts with a typical thermodynamic preference for self-sorting.

Aromatic identity, electronic substitution, and sequence in amphiphilic tripeptide self-assembly.

The phenomenon of self-assembly in short peptides (2-4 amino acids) has been a source of curiosity, in part for its role in helping to better understand and predict how minimal sequences within proteins might contribute to the formation of larger structures or aggregates. Building on previous work in this field, here we investigate a family of amphiphilic tripeptides for their self-assembly and hydrogel formation. From a parent peptide, Ac-FID-NH2, which was previously shown to self-assemble into high aspect-ratio filaments and form hydrogels, we explored the significance of structural features or sequence variations on the observed self-assembly.

Self-assembly of amphiphilic tripeptides with sequence-dependent nanostructure.

Supramolecular chemistry enables the creation of a diversity of nanostructures and materials. Many of these have been explored for applications as biomaterials and therapeutics. Among them, self-assembling peptides have been broadly applied.