Publications by authors named "Calvert L Green"
Among the micronutrients required by humans, zinc has particularly divergent modes of action. cDNA microarray and quantitative PCR technologies were used to investigate the zinc responsiveness of known genes that influence zinc homeostasis and to identify, through global screening, genes that may relate to phenotypic outcomes of altered dietary zinc intake. Human monocytic/macrophage THP-1 cells were either acutely zinc depleted, using a cell-permeable zinc-specific chelator, or were supplemented with zinc to alter intracellular zinc concentrations.
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- Researchers used differential mRNA display and cDNA array analysis to identify genes regulated by zinc in the small intestine, thymus, and monocytes, revealing most of the transcriptome is unaffected by dietary zinc.
- Key zinc-regulated genes linked to the immune response, stress response, growth, and energy metabolism were identified, including uroguanylin and various zinc transporters (ZnT-1, ZnT-2, ZnT-4).
- ZnT transporters play crucial roles in zinc acquisition and storage, with their expression varying significantly during pregnancy and lactation, while studies in THP-1 cells highlighted how zinc status influences transporter expression.
Cysteine-rich intestinal protein (CRIP), which contains a double zinc finger motif, is a member of the Group 2 LIM protein family. Our results showed that the developmental regulation of CRIP in neonates was not influenced by conventional vs. specific pathogen-free housing conditions.
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