A Novel Antigen Design Strategy to Isolate Single-Domain Antibodies that Target Human Nav1.7 and Reduce Pain in Animal Models.

Genetic studies have identified the voltage-gated sodium channel 1.7 (Na1.7) as pain target.

Missed opportunities for human papillomavirus vaccination using Iowa's Immunization Registry Information System.

Purpose: Adolescent human papillomavirus (HPV) vaccination rates continue to remain lower than other adolescent vaccines, both nationwide and in Iowa. This study examined predictors of missed opportunities for first-dose HPV vaccine administrations in Iowa in order to conduct more targeted outreach and improve adolescent HPV vaccine uptake.

Methods: A retrospective study was conducted to identify predictors of missed opportunities for first-dose HPV vaccination in Iowa adolescents using Iowa's Immunization Registry Information System.

Benefits and Challenges of Sealing Deceased Individual Immunization Records in Immunization Information Systems (IIS): An Overview of Iowa's Process.

Data cleansing practices aimed to improve data quality in immunization information systems (IIS) continue to be identified and evaluated by immunization programs to generate accurate and reliable immunization coverage rates. The Iowa Immunization Program has implemented several automated, daily data cleansing practices to improve the quality of records in Iowa's Immunization Registry Information System (IRIS), including the process of sealing records of deceased individuals through vital records matching. This process removes deceased individual records from the active IIS population, which helps reduce denominator inflation and improve the accuracy of immunization rate calculations.

A multilevel conceptual framework on green practices: Transforming policies into actionable leadership and employee behavior.

As organizations have recognized their cause/solution relationship with the environment, increasing attention is being given to the role of employees make in achieving green organizational objectives. Even though, business sustainability initiatives are often led by leaders; employee green behavior (EGB) plays a vital role in success of such initiatives. The current paper focuses on relatively less researched topic of EGB.

A comparison of activity demands between trial matches and in-season matches across multiple teams and seasons in semi-professional, male rugby league players.

Trial matches are frequently used for team preparation in rugby league competitions, making it essential to understand the demands experienced to assess their specificity to actual competition. Consequently, this study aimed to compare the activity demands between pre-season trial matches and early in-season rugby league matches. Following a repeated-measures observational design, 39 semi-professional, male rugby league players from two clubs were monitored using microsensors during two trial matches and the first two in-season matches across two consecutive seasons.

Porocarcinoma of the Left Foot: A Case Review.

Eccrine porocarcinoma is a rare malignant tumor of the eccrine sweat gland. This malignancy occurs most commonly in the lower extremities. It tends to occur in patients aged 60 to 80 years, affecting men and women equally.

Cell surface profiling of cultured cells by direct hydrazide capture of oxidized glycoproteins.

Glycoproteins are a particularly interesting subset of the cellular proteome as a high proportion of proteins present on the extracellular cell surface are glycosylated. These cell surface proteins are ideal targets for biologic drug therapies or for diagnostics tests. Here, we describe a modification of the well-described Cell Surface Capture (CSC) method for the selective isolation and identification of cell surface glycoproteins that contain N-linked carbohydrates.

Assessing coastline recession for adaptation planning: sea level rise versus storm erosion.

The Sixth Assessment report (AR6) of the Intergovernmental Panel on Climate Change (IPCC) states with high confidence that most sandy coasts around the world will experience an increase in coastal erosion over the twenty-first century. An increase in long term coastal erosion (coastline recession) along sandy coasts can translate into massive socio-economic impacts, unless appropriate adaptation measures are implemented in the next few decades. To adequately inform adaptation measures, it is necessary to have a good understanding of the relative importance of the physical processes driving coastline recession, as well as of linkages between consideration (or not) of certain processes and the level of risk tolerance; understandings that are hitherto lacking.

Immunoassay for Quantitative Detection of Antibody Transcytosis Across the Blood-Brain Barrier In Vitro.

Automated high-throughput immunoassays are emerging as reliable analytic techniques for the quantitative detection of proteins from a variety of sample types. Herein, we describe a method using the Protein Simple Wes capillary-based automated immunoassays platform for the quantification of His- and HA-tagged antibody transcytosis across an in vitro transwell blood-brain barrier (BBB) model. Compared to conventional ELISA, fluorescence, and Mass Spec-based detection approaches, Wes provides comparable datasets with additional information regarding size, aggregation, and potential degradation of samples before and after BBB transcytosis.

Validity of the PUSH band 2.0 and Speed4lifts to measure velocity during upper and lower body free-weight resistance exercises.

Accuracy and test-retest reliability were assessed for two devices, PUSH Band 2.0 (PUSH) and Speed4lifts. Two identical sessions were performed 6-8 days apart.

Probabilistic projections of the stability of small tidal inlets at century time scale using a reduced complexity approach.

Climate change is widely expected to affect the thousands of small tidal inlets (STIs) dotting the global coastline. To properly inform effective adaptation strategies for the coastal areas in the vicinity of these inlets, it is necessary to know the temporal evolution of inlet stability over climate change time scales (50-100 years). As available numerical models are unable to perform continuous morphodynamic simulations at such time scales, here we develop and pilot a fast, probabilistic, reduced complexity model (RAPSTA - RAPid assessment tool of inlet STAbility) that can also quantify forcing uncertainties.

Coral composition and bottom-wave metrics improve understanding of the patchiness of cyclone damage on reefs.

Coral reefs are likely to be exposed to more intense cyclones under climate change. Cyclone impacts are spatially highly variable given complex hydrodynamics, and coral-specific sensitivity to wave impacts. Predicting reef vulnerability to cyclones is critical to management but requires high resolution environmental data that are difficult to obtain over broad spatial scales.

Barriers and facilitators to implementing community-based physical activity interventions: a qualitative systematic review.

Background: Over the past decade several physical activity (PA) interventions have been shown to be efficacious in a controlled research setting, however there is a continued lack of evidence for how to successfully implement these PA interventions in real-world settings such as the community. This review aims to explore the barriers and facilitators that affect the implementation of community-based PA interventions and make recommendations to improve implementation from the included studies.

Methods: A systematic literature search of EBSCOhost, Scopus, PUBMED and Web of Science was conducted to identify articles that reported qualitative data on the implementation factors of community-based interventions where PA was a primary outcome.

Health Promoting Sports Club in Practice: A Controlled Evaluation of the GAA Healthy Club Project.

Sport is a developing setting and a relevant system in health promotion but there are few examples of settings-based initiatives and systems thinking in sport. The Gaelic Athletic Association (GAA) Healthy Club Project (HCP) adopts a settings approach delivered through and by grassroots clubs who respond to local needs while working within a national support system. The aim of this evaluation was to assess and describe the health promotion impact and experience of the HCP.

LightBox: A multiwell plate illumination system for photoactive molecule characterization.

Multiwell plates (MWPs) are the workhorses of the life sciences. However, biophotonics research with MWPs is limited, in part due to the lack of equipment suitable for photo-irradiation of photoactive molecules in a MWP-suitable, high-throughput manner, either commercially or through open-source MWP systems. Here we present "LightBox", a calibrated controllable MWP illumination system with broad applications including screening of photoactive molecules and characterization of photocatalytic chemicals.

Multi-parametric analysis of 57 SYNGAP1 variants reveal impacts on GTPase signaling, localization, and protein stability.

SYNGAP1 is a neuronal Ras and Rap GTPase-activating protein with important roles in regulating excitatory synaptic plasticity. While many SYNGAP1 missense and nonsense mutations have been associated with intellectual disability, epilepsy, schizophrenia, and autism spectrum disorder (ASD), whether and how they contribute to individual disease phenotypes is often unknown. Here, we characterize 57 variants in seven assays that examine multiple aspects of SYNGAP1 function.

Validity and Reliability of Using Load-Velocity Relationship Profiles to Establish Back Squat 1 m·s-1 Load.

Elsworthy, N, Callaghan, DE, Scanlan, AT, Kertesz, AHM, Kean, CO, Dascombe, BJ, and Guy, JH. Validity and reliability of using load-velocity relationship profiles to establish back squat 1 m·s-1 load. J Strength Cond Res 35(2): 340-346, 2021-Although measuring movement velocity during resistance exercise is being increasingly used to monitor player readiness for competition in team sports, the validity and reliability of using set target velocities has not been examined.

Synthesising wave attenuation for seagrass: Drag coefficient as a unifying indicator.

An estimated 100 million people inhabit coastal areas at risk from flooding and erosion due to climate change. Seagrass meadows, like other coastal ecosystems, attenuate waves. Due to inconsistencies in how wave attenuation is measured results cannot be directly compared.

Cellular localisation of structurally diverse diphenylacetylene fluorophores.

Fluorescent probes are increasingly used as reporter molecules in a wide variety of biophysical experiments, but when designing new compounds it can often be difficult to anticipate the effect that changing chemical structure can have on cellular localisation and fluorescence behaviour. To provide further chemical rationale for probe design, a series of donor-acceptor diphenylacetylene fluorophores with varying lipophilicities and structures were synthesised and analysed in human epidermal cells using a range of cellular imaging techniques. These experiments showed that, within this family, the greatest determinants of cellular localisation were overall lipophilicity and the presence of ionisable groups.

Back squat velocity to assess neuromuscular status of rugby league players following a match.

Objectives: Back squat mean concentric velocity (MV) and countermovement jump (CMJ) performance were examined in sub-elite rugby league players post-match to monitor changes in neuromuscular status (NMS) from baseline. Relationships between changes in back squat MV and CMJ performance variables were used to compare back squat MV to an established method to monitor NMS.

Design: Longitudinal observational design.

Seagrass losses since mid-20th century fuelled CO emissions from soil carbon stocks.

Seagrass meadows store globally significant organic carbon (C ) stocks which, if disturbed, can lead to CO emissions, contributing to climate change. Eutrophication and thermal stress continue to be a major cause of seagrass decline worldwide, but the associated CO emissions remain poorly understood. This study presents comprehensive estimates of seagrass soil C erosion following eutrophication-driven seagrass loss in Cockburn Sound (23 km between 1960s and 1990s) and identifies the main drivers.

Multi-model functionalization of disease-associated PTEN missense mutations identifies multiple molecular mechanisms underlying protein dysfunction.

Functional variomics provides the foundation for personalized medicine by linking genetic variation to disease expression, outcome and treatment, yet its utility is dependent on appropriate assays to evaluate mutation impact on protein function. To fully assess the effects of 106 missense and nonsense variants of PTEN associated with autism spectrum disorder, somatic cancer and PTEN hamartoma syndrome (PHTS), we take a deep phenotypic profiling approach using 18 assays in 5 model systems spanning diverse cellular environments ranging from molecular function to neuronal morphogenesis and behavior. Variants inducing instability occur across the protein, resulting in partial-to-complete loss-of-function (LoF), which is well correlated across models.