Exploring the Relationship Between Humoral and Cellular T Cell Responses Against SARS-CoV-2 in Exposed Individuals From Emilia Romagna Region and COVID-19 Severity.

COVID-19 remains a significant global health problem with uncertain long-term consequences for convalescents. We investigated the relationships between anti-N protein antibody levels, severe acute respiratory syndrome (SARS)-CoV-2-associated TCR repertoire parameters, HLA type and epidemiological information from three cohorts of 524 SARS-CoV-2-infected subjects subgrouped in acute phase, seronegative and seropositive convalescents from the Emilia Romagna region. Epidemiological information and anti-N antibody index were associated with TCR repertoire data.

Discovery of a pan anti-SARS-CoV-2 monoclonal antibody with highly efficient infected cell killing capacity for novel immunotherapeutic approaches.

Unlocking the potential of broadly reactive coronavirus monoclonal antibodies (mAbs) and their derivatives offers a transformative therapeutic avenue against severe COVID-19, especially crucial for safeguarding high-risk populations. Novel mAb-based immunotherapies may help address the reduced efficacy of current vaccines and neutralizing mAbs caused by the emergence of variants of concern (VOCs). Using phage display technology, we discovered a pan-SARS-CoV-2 mAb (C10) that targets a conserved region within the receptor-binding domain (RBD) of the virus.

High-fiber basil seed flour reduces insulin resistance and hepatic steatosis in high-fat diet mice.

The incidence of insulin resistance (IR) and hepatic steatosis is increasing, with dietary fiber playing a protective role against these disorders. Ocimum basilicum L., widely used in food, pharmaceutical, and cosmetic industries, but their health-promoting properties remain underexplored.

The association between intraoperative low driving pressure ventilation and perioperative healthcare-associated costs: A retrospective multicenter cohort study.

Study Objective: A low dynamic driving pressure during mechanical ventilation for general anesthesia has been associated with a lower risk of postoperative respiratory complications (PRC), a key driver of healthcare costs. It is, however, unclear whether maintaining low driving pressure is clinically relevant to measure and contain costs. We hypothesized that a lower dynamic driving pressure is associated with lower costs.

Highly efficient mobility, separation and charge transfer in black SnO-TiO structures with co-catalysts: the key step for the photocatalytic hydrogen evolution.

Oxygen vacancies and co-catalysts enhance photocatalytic hydrogen production by improving the charge carrier separation. Herein, the black SnO-TiO structure (BST) was synthesized for the first time by two consecutive methods. First, the sol-gel nucleation method allowed TiO to form on the SnO nanoparticles, creating a strong interaction and direct contact between them.

CAR affinity modulates the sensitivity of CAR-T cells to PD-1/PD-L1-mediated inhibition.

Chimeric antigen receptor (CAR)-T cell therapy for solid tumors faces significant hurdles, including T-cell inhibition mediated by the PD-1/PD-L1 axis. The effects of disrupting this pathway on T-cells are being actively explored and controversial outcomes have been reported. Here, we hypothesize that CAR-antigen affinity may be a key factor modulating T-cell susceptibility towards the PD-1/PD-L1 axis.

Cellular and humoral responses after second and third SARS-CoV-2 vaccinations in patients with autoimmune diseases treated with rituximab: specific T cell immunity remains longer and plays a protective role against SARS-CoV-2 reinfections.

Background: Humoral and cellular immune responses are known to be crucial for patients to recover from COVID-19 and to protect them against SARS-CoV-2 reinfection once infected or vaccinated.

Objectives: This study aimed to investigate humoral and T cell responses to SARS-CoV-2 vaccination in patients with autoimmune diseases after the second and third vaccine doses while on rituximab and their potential protective role against reinfection.

Methods: Ten COVID-19-naïve patients were included.

Tautomerism unveils a self-inhibition mechanism of crystallization.

Modifiers are commonly used in natural, biological, and synthetic crystallization to tailor the growth of diverse materials. Here, we identify tautomers as a new class of modifiers where the dynamic interconversion between solute and its corresponding tautomer(s) produces native crystal growth inhibitors. The macroscopic and microscopic effects imposed by inhibitor-crystal interactions reveal dual mechanisms of inhibition where tautomer occlusion within crystals that leads to natural bending, tunes elastic modulus, and selectively alters the rate of crystal dissolution.

CoVITEST: A Fast and Reliable Method to Monitor Anti-SARS-CoV-2 Specific T Cells From Whole Blood.

Cellular and humoral immune responses are essential for COVID-19 recovery and protection against SARS-CoV-2 reinfection. To date, the evaluation of SARS-CoV-2 immune protection has mainly focused on antibody detection, generally disregarding the cellular response, or placing it in a secondary position. This phenomenon may be explained by the complex nature of the assays needed to analyze cellular immunity compared with the technically simple and automated detection of antibodies.

Integration of Highly Luminescent Lead Halide Perovskite Nanocrystals on Transparent Lead Halide Nanowire Waveguides through Morphological Transformation and Spontaneous Growth in Water.

The integration of highly luminescent CsPbBr quantum dots on nanowire waveguides has enormous potential applications in nanophotonics, optical sensing, and quantum communications. On the other hand, CsPb Br nanowires have also attracted a lot of attention due to their unique water stability and controversial luminescent property. Here, the growth of CsPbBr nanocrystals on CsPb Br nanowires is reported first by simply immersing CsPbBr powder into pure water, CsPbBr  X (X = Cl, I) nanocrystals on CsPb Br  X nanowires are then synthesized for tunable light sources.

Home care assistants' attitudes and perceptions of caring for people at the end of life in their homes in Sweden.

The ageing population is increasing worldwide, with older people often having multimorbidity and a need for help with activities and personal care. Home Care Assistants (HCAs) are central to the provision of care in the home. They meet older people approaching the end of life and their relatives.

Effect of fiber source and crude protein level on nursery pig performance and fecal microbial communities.

Reduction in dietary crude protein and addition of fiber could mitigate the incidence and severity of post-weaning diarrhea, a common gastrointestinal condition in newly weaned pigs. Therefore, 360 weanling pigs, initially 5.0 ± 0.

Evaluation of microencapsulated organic acids and botanicals on growth performance of nursery and growing-finishing pigs.

A total of 1,215 pigs (L337 × 1050, PIC, Hendersonville, TN) were used to determine the effect of microencapsulated organic acids and botanicals (MOB; AviPlus; Vetagro, Inc. Chicago, IL), on growth performance from weaning to market. Pigs were weaned at approximately 21 d of age and placed in pens based on initial body weight (BW) with 27 pigs per pen in a randomized complete block design.

Live yeast and yeast extracts with and without pharmacological levels of zinc on nursery pig growth performance and antimicrobial susceptibilities of fecal Escherichia coli.

A total of 360 weanling barrows (Line 200 ×400, DNA, Columbus NE; initially 5.6 ± 0.03 kg) were used in a 42-d study to evaluate yeast-based pre- and probiotics (Phileo by Lesaffre, Milwaukee, WI) in diets with or without pharmacological levels of Zn on growth performance and antimicrobial resistance (AMR) patterns of fecal Escherichia coli.

The effects of pharmacological levels of zinc, diet acidification, and dietary crude protein on growth performance in nursery pigs.

This experiment was conducted to evaluate potential replacements for pharmacological levels of Zn (provided by Zn oxide), such as diet acidification (sodium diformate) and low dietary crude protein (CP: 21 vs 18%) on nursery pig performance and fecal dry matter (DM). A total of 360 weaned pigs (Line 200 × 400, DNA, Columbus, NE; initially 5.90 ± 0.

Onasemnogene Abeparvovec (AVXS-101) for the Treatment of Spinal Muscular Atrophy.

Spinal muscular atrophy (SMA) is a debilitating disorder characterized by degeneration of large motor neurons. It is a heterogeneous group of disorders caused by a homozygous deletion in the survival motor neuron () gene on chromosome 5, resulting in a SMN protein deficiency. Small amounts of SMN protein are also produced by the gene, which that differs from by a single nucleotide.

Effect of high-phytase supplementation in lactation diets on sow and litter performance.

A total of 109 sows (Line 241; DNA, Columbus, NE) were used to evaluate the effect of increasing dietary phytase in lactation diets, already adequate in P, on sow and litter performance. On d 107 of gestation, sows were blocked by body weight and parity and allotted to 1 of 3 dietary treatments of increasing phytase concentration (0, 1,000, or 3,000 FYT/kg; Ronozyme HiPhos GT 2700, DSM Nutritional Products, Inc., Parsippany, NJ).

Efficacy of commercial products on nursery pig growth performance fed diets with fumonisin contaminated corn.

Two experiments were conducted to determine the efficacy of various commercial products on growth performance of nursery pigs fed diets high in fumonisin. In experiment 1, 350 pigs (241 × 600; DNA, Columbus, NE; initially 9.9 kg) were used with five pigs per pen and 14 replicates per treatment.

Photocatalytic behavior for the phenol degradation of ZnAl layered double hydroxide functionalized with SDS.

Functionalized ZnAl layered double hydroxide based photocatalyst was obtained by the addition of sodium dodecyl sulfate (SDS) during the synthesis by the coprecipitation method, and further calcination at 400 °C. Bare and modified materials were characterized by X-ray diffraction, nitrogen adsorption-desorption, IR, UV-Vis, EPR and XPS spectroscopies, SEM and HRTEM. The synthesized material was evaluated in the photodegradation of phenol in a 40 ppm aqueous solution (4.

Effects of Fumonisin-Contaminated Corn on Growth Performance of 9 to 28 kg Nursery Pigs.

Fumonisin contamination in corn is an emerging issue in animal feed production. Fumonisin disrupts the metabolism of sphingolipids and reduces growth performance. This experiment was conducted to determine the effect of feeding fumonisin-contaminated corn on growth performance and sphinganine (SA) to sphingosine (SO) ratios of 9 to 28 kg pigs.

Triple-negative breast cancer in Peru: 2000 patients and 15 years of experience.

Background: Epidemiological studies commonly identify the clinical characteristics and survival outcomes of patients with breast cancer at five years. Our study aims to describe the sociodemographic, clinicopathological characteristics and determine the long-term event-free survival (EFS) and overall survival (OS) of a Peruvian population with triple-negative breast cancer.

Methods: We reviewed the medical records of new cases treated at a single institution in the period 2000-2014.

Sow and piglet traits associated with piglet survival at birth and to weaning.

AbstractUnderstanding the relationship between sow and piglet characteristics that are associated with stillborn rate and preweaning mortality is beneficial as litter size continues to increase. Two experiments were previously conducted to evaluate prefarrowing nutrition regimens on sow and litter characteristics. These two datasets (experiments 1 and 2) were then used to identify sow and piglet characteristics associated with stillborn rate and piglet survival to weaning.