Publications by authors named "Caitlin R Russell"
Article Synopsis
- Mutations in the RBM20 cardiac splicing factor are linked to malignant dilated cardiomyopathy (DCM), and engineered isogenic iPSCs were used to study this condition.
- iPSC-derived heart tissues displayed contractile dysfunction, with missense mutations resulting in more severe effects compared to RBM20 knockout lines.
- Analysis of the mutant RBM20 revealed altered RNA binding, unique gene expression defects, and a tendency to localize in the cytoplasm with stress granule interactions, indicating a complex mechanism underlying cardiac disease.
Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales.
Nathaniel Huebsch Peter Loskill Mohammad A Mandegar Natalie C Marks Alice S Sheehan Caitlin R Russell
Tissue Eng Part C Methods
May 2015
Contractile motion is the simplest metric of cardiomyocyte health in vitro, but unbiased quantification is challenging. We describe a rapid automated method, requiring only standard video microscopy, to analyze the contractility of human-induced pluripotent stem cell-derived cardiomyocytes (iPS-CM). New algorithms for generating and filtering motion vectors combined with a newly developed isogenic iPSC line harboring genetically encoded calcium indicator, GCaMP6f, allow simultaneous user-independent measurement and analysis of the coupling between calcium flux and contractility.
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