Prior-Night Sleep Predicts Next-Day Sedentary Behavior as a Function of Work Duration in Public Safety Telecommunicators.

Public safety telecommunicators are at significant risk for obesity and other health conditions, which can likely be attributed to high-intensity job functions that occur in seated positions for long work hours. However, the interactions between these variables, as well as the role of recovery sleep, remains underexplored. We hypothesized that work duration would moderate the daily relationship between prior-night sleep and next day sedentary behavior.

A simple and highly reproducible method for the detection of erythrocyte membrane ZIP metal transporters by immunoblotting.

Manganese is one of the essential trace elements found in erythrocytes. Metal transporters situated on the plasma membrane generally facilitate the movement of manganese into and out of cells. This study aims at determining whether two recently discovered manganese importers, ZIP8 and ZIP14, are located in the erythrocyte membrane.

The Combined Inactivation of Intestinal and Hepatic ZIP14 Exacerbates Manganese Overload in Mice.

ZIP14 is a newly identified manganese transporter with high levels of expression in the small intestine and the liver. Loss-of-function mutations in can lead to systemic manganese overload, which primarily affects the central nervous system, causing neurological disorders. To elucidate the roles of intestinal ZIP14 and hepatic ZIP14 in maintaining systemic manganese homeostasis, we generated mice with single-tissue or two-tissue knockout, including intestine-specific (-In-KO), liver-specific (-L-KO), and double (intestine and liver) -knockout (-DKO) mice.