Physiological and transcriptome analysis of sex-specific responses to cadmium stress in poplars.

Soil cadmium (Cd) pollution is a serious ecological problem worldwide. Understanding Cd-detoxification mechanisms in woody plants will help to evaluate their tolerance ability and phytoremediation potential to Cd-polluted soils. This study investigated the growth, physiochemistry, Cd distribution, and transcriptome sequencing of male and female poplars under three Cd levels (0, 50, and 100 mg·kg).

Enzyme-assisted Rosa davurica mitigates UV-induced skin photodamage by modulating apoptosis through Nrf2/ARE and MAPK/NF-κB pathways.

Exposure to UV irradiation results in abnormal, extensive apoptosis of skin cells. This excessive cell death can promote inflammation and alter the microenvironment, increasing the risk of skin cancer. Despite extensive research, few materials are effective at simultaneously protecting against both UVA and UVB irradiation.

Biomimetic nanostructural materials based on placental amniotic membrane-derived nanofibers for self-healing and anti-adhesion during cesarean section.

Cesarean section (CS) is highly prevalent surgery among females. However, current absorbable anti-adhesion membranes used clinically can partially prevent postoperative adhesions but show limited efficacy in tissue regeneration, leaving post-cesarean women at risk for severe complications including cesarean scar pregnancy, placenta previa, and uterine rupture. Herein, we designed a fully amniotic membrane (AM)-derived biomimetic nanostructural materials (AM-BNMs) as an anti-adhesion barrier, and validated its therapeutic efficacy in a rat CS model.

AIScholar: An OpenFaaS-enhanced cloud platform for intelligent medical data analytics.

This paper presents AIScholar, an intelligent research cloud platform developed based on artificial intelligence analysis methods and the OpenFaaS serverless framework, designed for intelligent analysis of clinical medical data with high scalability. AIScholar simplifies the complex analysis process by encapsulating a wide range of medical data analytics methods into a series of customizable cloud tools that emphasize ease of use and expandability, within OpenFaaS's serverless computing framework. As a multifaceted auxiliary tool in medical scientific exploration, AIScholar accelerates the deployment of computational resources, enabling clinicians and scientific personnel to derive new insights from clinical medical data with unprecedented efficiency.

Facial Thread Lifting Complications: Underlying Causes.

Introduction: Thread lifting procedures are becoming increasingly popular for facial rejuvenation. However, various complications can arise during these procedures, many of which are influenced by anatomic factors.

Methods: In this section, the authors explore the potential side effects associated with thread lifting and emphasize the anatomic structures that require careful attention.

Characterization of Key Lipid Components in the Cell Membrane of Freeze-Drying Resistant Strains Using Nontargeted Lipidomics.

Lactic acid bacteria (LAB) are usually freeze-dried into powder for transportation and storage, with the bacterial membrane playing a crucial role in this process. However, different strains exhibit different levels of freeze-drying resistance in their cell membranes. In this study, () strains 1F20, K56, and J5, demonstrating survival rates of 59.

Injection Techniques for Filler Procedures With Illustration: Narrative Review.

This narrative review explores the various injection techniques utilized in facial filler procedures, emphasizing their applications, benefits, and potential complications. The increasing demand for total facial contouring and rejuvenation has led to a rise in the use of volumizing fillers and the integration of these procedures with other treatments such as toxins, threads, lasers, radiofrequency, and ultrasound. Achieving satisfactory results necessitates a comprehensive understanding of facial anatomy and the effects of aging on skin tissue.

Upper Lip Single-Point Technique for Lip Reshaping: A Safe, Minimalistic Approach in Over 500 Cases-A Retrospective Study.

Lip augmentation has become one of the most popular esthetic procedures globally, driven by societal standards that equate full lips with youth and sensuality. While young individuals often seek volume enhancement for lip beautification, older adults pursue lip rejuvenation to counter aging effects, such as volume loss, fine lines, and reduced definition. A range of techniques has been developed to meet these esthetic goals, but achieving natural and safe results remains a challenge.

Single-cell and spatial transcriptomic analyses reveal heterogeneity characteristics and specific cell subtype regulators in growth hormone-secreting pituitary adenomas.

Background: Growth hormone-secreting pituitary adenomas (GHPA) display diverse biological behaviors and clinical outcomes, necessitating the identification of tumor heterogeneity and prognostically relevant markers.

Methods: In this study, we performed single-cell RNA sequencing (scRNA-seq) on 10 GHPA samples, four of which also underwent spatial transcriptome sequencing, and used scRNA-seq data from four normal pituitary samples as controls. Cell subtype characterization in GHPA was analyzed using multiple algorithms to identify malignant bias regulators, which were then validated using a clinical cohort.

Simultaneous Blockade of CD209 and CD209L by Monoclonal Antibody Does Not Provide Sufficient Protection Against Multiple Viral Infections In Vivo.

Many virus species, including Ebola virus, Marburg virus, SARS-CoV-2, dengue virus (DENV) and Zika virus (ZIKV), exploit CD209 and CD209L as alternative or attachment receptors for viral cis- or trans-infection. Thus, CD209 and CD209L may be critical targets for the development of therapeutic monoclonal blocking antibody drugs to disrupt the infection process caused by multiple viruses. Here, we produced a human chimeric monoclonal blocking antibody that simultaneously blocks CD209 and CD209L, namely 7-H7-B1.

A Nomogram Model for Predicting Recurrent Coronary Thrombosis in Kawasaki Disease Patients.

Background: Coronary thrombosis is a serious cardiovascular complication of Kawasaki disease (KD), and recurrence of coronary thrombosis increases the short-term risk of myocardial infarction and the long-term risk of coronary artery disease. However, there are currently no studies predicting the recurrence of coronary thrombosis, so the aim of this study was to develop and validate a nomogram to predict recurrent coronary thrombosis in KD patients.

Methods: This was a retrospective study of data from 149 KD patients who had a history of previous coronary disease at the Children's Hospital of Chongqing Medical University from 2013 to 2020.

Efficacy and Renal Safety of Protocol-based 11.7% Hypertonic Saline Infusion Compared with 20% Mannitol in Patients with Elevated Intracranial Pressure: A Study Protocol for a Randomized Clinical Trial.

Background: Elevated intracranial pressure (ICP) is a potentially life-threatening condition requiring prompt intervention. While both mannitol and hypertonic saline (HTS) are commonly used hyperosmotic agents for treating elevated ICP, there is insufficient evidence comparing their renal safety profiles and overall effectiveness. This study protocol outlines a pragmatic randomized trial to compare protocol-based 11.

g-CN S-Scheme Homojunction through Van der Waals Interface Regulation by Intrinsic Polymerization Tailoring for Enhanced Photocatalytic H Evolution and CO Reduction.

The effective S-scheme homojunction relies on the precise regulation of band structure and construction of advantaged charge migration interfaces. Here, the electronic structural properties of g-CN were modulated through meticulous polymerization of self-assembled supramolecular precursors. Experimental and DFT results indicate that both the intrinsic bandgap and surface electronic characteristics were adjusted, leading to the formation of an in-situ reconstructed homojunction interface facilitated by intrinsic van der Waals forces.

Exogenously and Endogenously Sequential Regulation of DNA Nanodevices Enables Organelle-Specific Signal Amplification in Subcellular ATP Profiling.

Adenosine triphosphate (ATP), the primary energy currency in cells, is dynamically regulated across different subcellular compartments. The ATP interplay between mitochondria and endoplasmic reticulum (ER) underscores their coordinated roles in various biochemical processes, highlighting the necessity for precise profiling of subcellular ATP dynamics. Here we present an exogenously and endogenously dual-regulated DNA nanodevice for spatiotemporally selective, subcellular-compartment specific signal amplification in ATP sensing.

The Role of Immunotherapy in Lung Cancer Treatment: Current Strategies, Future Directions, and Insights into Metastasis and Immune Microenvironment.

Lung cancer is a leading cause of mortality worldwide. Immunotherapy has emerged as a potentially effective strategy, as traditional medicines have shown minimal success. This review investigates the current state of immunotherapy for lung cancer treatment, focusing on its mechanisms, clinical applications, strategies, and future directions.

Enhancing fermented vegetable flavor with Lactobacillus plantarum and Rhodotorula mucilaginosa.

The formation of flavor in fermented vegetables is directly associated with the interactions among the resident microbial strains. This study explored the cooperative dynamics between Lactobacillus plantarum and Rhodotorula mucilaginosa in a simulated cabbage juice system. The obtained results indicated that the co-cultivation of these strains accelerated fermentation kinetics and enhanced lactic acid production.

Characterization of perceptual interactions among aroma compounds found in Rose damascena and Angelica dahurica root essential oil with threshold, S-curve, σ-τ plot and molecular docking.

The study investigated the perceptual interaction between two types of Rose damascena essential oil and two types of Angelica dahurica root essential oil. Using gas chromatography-olfactometer (GC-O) and gas chromatography-mass spectrometer (GC-MS), 24 and 25 aromatic compounds in Rose damascena essential oil and Angelica dahurica root essential oil were identified and quantified, respectively. Based on flavor dilution (FD) values and odor activity values (OAVs), 10 important aroma compounds in Rose damascena essential oil and 6 in Angelica dahurica root essential oil were identified.

Integrated omics analysis reveals the interactions among ROS metabolism and malate metabolism associated with chilling tolerance in peach fruit treated with hot air.

Continuous supply of NADPH is necessary for the synthesis of ROS, which can be derived from the decarboxylation of malic acid, providing fuels for RbOHs to sustain ROS production. However, excessive accumulations of ROS lead to significant chilling injury (CI) in peaches during cold storage. Our previous studies indicated that hot air (HA) slows the CI progression in peaches by preventing malate degradation.

Artificial Intelligence Models May Aid in Predicting Lymph Node Metastasis in Patients with T1 Colorectal Cancer.

Background/aims: Inaccurate prediction of lymph node metastasis (LNM) may lead to unnecessary surgery following endoscopic resection of T1 colorectal cancer (CRC). We aimed to validate the usefulness of artificial intelligence (AI) models for predicting LNM in patients with T1 CRC.

Methods: We analyzed the clinical data, laboratory results, pathological reports, and endoscopic findings of patients who underwent radical surgery for T1 CRC.

Race and Gender Bias in Narrative Letters of Recommendation for Plastic Surgery Residency Applicants.

Introduction: It has been previously shown that gender bias exists in standardized letters of recommendation for plastic surgery residency. However, similar analysis has not been conducted for narrative letters of recommendation (NLORs). Therefore, this study aims to determine if there exists linguistic bias in NLORs for plastic surgery residency based on applicants' and writers' gender and race.

Application of the nitrogen-to-argon ratio to understand nitrogen transformation pathways in landfills under in-situ stabilization.

The ratio of nitrogen (N) to argon (Ar) in landfill gas was compared to the atmospheric gas ratio to quantify the balance between N generating (anaerobic ammonium oxidation, denitrification) and N consuming (nitrogen fixation) processes on three landfills undergoing in-situ stabilization. In the aerated landfills, as much as 22% of the extracted N could be explained by net denitrification, with coexisting aerobic and anaerobic domains fostering nitrification-dependent denitrification. Nitrogen fixation was also occasionally observed.

Anti-Inflammatory Withanolides From Physalis angulata Var. villosa Bonati.

Two new withanolide derivatives (1 and 2) and 12 known withanolide analogs (3-14) were identified from the aerial part of Physalis angulata var. villosa Bonati. Their structures were determined by various spectral data, quantum chemical calculations, and sugar derivatization methods.