The weak association between hypoxia tolerance and thermal tolerance increases the susceptibility of abalone to climate change.

The simultaneous occurrence of high temperatures and hypoxia events caused mass die-offs of aquatic animals. It is crucial to investigate the relationship between hypoxia tolerance and thermal tolerance of aquatic animals to predict the biological and ecological outcomes under global climate change scenarios. In this study, the hypoxia tolerance and thermal tolerance of Pacific abalone, Haliotis discus hannai, were measured by methods based on adhesion capacity (hypoxia adhesion duration and heat adhesion duration) and heart rate fluctuations (breakpoint of dissolved oxygen and Arrhenius breakpoint temperature).

Chromosome-level genome assembly of the ivory shell Babylonia areolata.

The ivory shell Babylonia areolata is an economically important marine benthic gastropod known for its rapid growth and high nutritional value. B. areolata is distributed in Southeast Asia and the southeast coastal areas of China.

Structure and function of vasa gene in gonadal gametogenesis of Pacific abalone.

Pacific abalone (Haliotis discus hannai) is a marine gastropod mollusc with significant economic importance in both global fisheries and aquaculture. However, studies exploring the gonadal development and regulatory mechanisms of Haliotis discus hannai are limited. This study aimed to explore whether the vasa gene acted as a molecular marker for germ cells.

Molecular Cloning, Characterization, and Function of Insulin-Related Peptide 1 (IRP1) in the .

Abalone is a popular mollusk in the marine aquaculture industry of China. However, existing challenges, like slow growth, individual miniaturization, and the absence of abundant abalone, have emerged as significant obstacles impeding its long-term progress in aquaculture. Studies have demonstrated that insulin-related peptide ) is a crucial factor in the growth of marine organisms.

TOmicsVis: An all-in-one transcriptomic analysis and visualization R package with Shinyapp interface.

Transcriptomic analysis has been widely used in comparative experiments to uncover biological mechanisms in various species. However, a simple tool is still lacking to optimize and integrate the features from multiple R packages. In this study, we developed TOmicsVis (Transcriptomics Visualization) (CRAN: https://cran.

Microbiota characterization of the green mussel at the tissue scale and its relationship with the environment.

Research on the microbiota associated with marine invertebrates is important for understanding host physiology and the relationship between the host and the environment. In this study, the microbiota of the green mussel was characterized at the tissue scale using 16S rRNA gene high-throughput sequencing and compared with the microbiota of the surrounding environment. Different mussel tissues were sampled, along with two environmental samples (the mussel's attachment substratum and seawater).

New genes helped acorn barnacles adapt to a sessile lifestyle.

Barnacles are the only sessile lineages among crustaceans, and their sessile life begins with the settlement of swimming larvae (cyprids) and the formation of protective shells. These processes are crucial for adaptation to a sessile lifestyle, but the underlying molecular mechanisms remain poorly understood. While investigating these mechanisms in the acorn barnacle, Amphibalanus amphitrite, we discovered a new gene, bcs-6, which is involved in the energy metabolism of cyprid settlement and originated from a transposon by acquiring the promoter and cis-regulatory element.

Preliminary analysis of pathways and their implications during salinity stress in abalone.

Transcriptome sequencing has offered immense opportunities to study non-model organisms. Abalone is an important marine mollusk that encounters harsh environmental conditions in its natural habitat and under aquaculture conditions; hence, research that increases molecular information to understand abalone physiology and stress response is noteworthy. Accordingly, the study used transcriptome sequencing of the gill tissues of abalone exposed to low salinity stress.

OmicsSuite: a customized and pipelined suite for analysis and visualization of multi-omics big data.

With the advancements in high-throughput sequencing technologies such as Illumina, PacBio, and 10X Genomics platforms, and gas/liquid chromatography-mass spectrometry, large volumes of biological data in multiple formats can now be obtained through multi-omics analysis. Bioinformatics is constantly evolving and seeking breakthroughs to solve multi-omics problems; however, it is challenging for most experimental biologists to analyse data using command-line interfaces, coding, and scripting. Based on experience with multi-omics, we have developed OmicsSuite, a desktop suite that comprehensively integrates statistics and multi-omics analysis and visualization.

Comparative Cytogenetic Analysis of Diploid and Triploid Pacific Abalone, Haliotis discus hannai.

Introduction: The Pacific abalone, Haliotis discus hannai, is one of the most commercially important marine shellfish in China. Cell engineering breeding is an important tool in abalone genetic breeding, and the triploids obtained through this method have high commercial value. However, current research mainly focuses on establishing induction methods and evaluating the growth traits of triploids, while there is a lack of basic research on triploid cytogenetics.

Impact of ocean acidification on shells of the abalone species Haliotis diversicolor and Haliotis discus hannai.

Ocean acidification (OA) results from the absorption of anthropogenic CO emissions by the ocean and threatens the survival of many marine calcareous organisms including molluscs. We studied OA effects on adult shells of the abalone species Haliotis diversicolor and Haliotis discus hannai that were exposed to three pCO conditions (ambient, ∼880, and ∼1600 μatm) for 1 year. Shell periostracum corrosion under OA was observed for both species.

Ecological benefits of artificial light at night (ALAN): Accelerating the development and metamorphosis of marine shellfish larvae.

Urbanization has led to increasing use of artificial light at night (ALAN), which has rapidly become an important source of pollution in many cities. To identify the ALAN effects on the embryonic development of the Pacific abalone Haliotis discus hannai, we first exposed larvae to natural light with a light period of 12 L:12D (control, Group CTR). We then exposed larvae to three different light regimes.

Comprehensive Comparison of Effects of Antioxidant (Astaxanthin) Supplementation from Different Sources in Diet.

Dietary antioxidant supplementation, especially astaxanthin, has shown great results on reproductive aspects, egg quality, growth, survival, immunity, stress tolerance, and disease resistance in aquatic animals. However, the effects of dietary astaxanthin supplementation from different sources are still unknown. A comprehensive comparison of survival, growth, immune response, antioxidant activity, thermal resistance, disease resistance, and intestinal microbial structure was conducted in dietary antioxidant supplementation from the sources of (GL), industrial synthetic astaxanthin (80 mg/kg astaxanthin actual weight, named as group 'SA80'), (80 mg/kg astaxanthin actual weight, named as group 'PR80') and (120 mg/kg astaxanthin actual weight, named as group 'HP120') at their optimal supplementation amounts.

 sp. nov. (Annelida, Hesionidae) from southeastern China.

(Annelida, Hesionidae) is described based on specimens collected from the coast of southeast China. It is the first species described from the Indo-Pacific, although there are already nine valid species known from other parts of the world. This new species can be distinguished from the other species in having a palpostyle as long as the palpophore and double aciculae in both notopodia and neuropodia, and in bearing bifid furcate chaetae which have a smooth base on the shorter tine.

Molecular Characterization and Function of Bone Morphogenetic Protein 7 (7) in the Pacific Abalone, .

Bone morphogenetic proteins (BMPs) play important roles in a lot of biological processes, such as bone development, cell proliferation, cell differentiation, growth, etc. However, the functions of abalone BMP genes are still unknown. This study aimed to better understand the characterization and biological function of BMP7 of (7) via cloning and sequencing analysis.

Micropodarke fujianensis n. sp. (Annelida: Hesionidae) from Fujian, China.

We describe M. fujianensis n. sp.

Metabolic and transcriptional responses demonstrating enhanced thermal tolerance in domesticated abalone.

Global warming threatens aquatic systems and organisms. Many studies have focused on the vulnerability and stress responses of aquaculture organisms to future thermal conditions. However, it may be of more practical significance to reveal their acclimation potential and mechanisms.

Mitochondrial genome of Kinberg, 1866 (Annelida: Hesionidae).

We report the complete mitochondrial genome of Kinberg, 1866 - the type species of the genus. It is 15061 bp long, and contains 13 protein-coding genes (PCGs), 22 tRNA genes (tRNAs), and 2 rRNA genes (rRNAs), and 1 putative control region. Phylogenetic analysis indicated that was placed as sister to (BS = 100) of the same family Hesionidae.

A Potential Negative Regulatory Function of Myostatin in the Growth of the Pacific Abalone, .

Myostatin, also known as GDF8, is a member of the transforming growth factor-β (TGF-β) superfamily. In vertebrates, myostatin negatively regulates the growth of skeletal muscle. In invertebrates, it has been reported to be closely related to animal growth.

Reproduction strategy of nocturnal marine molluscs: running for love.

The cost of reproduction is the core driver of life history evolution in animals. This paper demonstrates that the cumulative distance moved and the duration of movement of sexually immature abalones, Haliotis discus hannai, kept in various male and female groups, were significantly higher than those of sexually mature individuals, except when kept in mixed cultures of mature males and females. After mixed-culture, sexually mature males moved significantly further and for a longer duration than mature female abalones, and even more so than mature male abalones of any other group.

Structural characteristics of orexin receptor type 2 in Pacific abalone and its diurnal expression pattern after fasting and re-feeding.

Pacific abalone (Haliotis discus hannai) is a typical nocturnal organism. To examine the circadian expression pattern of orexin receptor type 2 (OXR) and its potential effect on the feeding behavior of abalone, the coding region sequence of OXR that is 1215 bp in length and encodes 404 amino acids was first cloned using the rapid amplification of cDNA ends technique. A recombinant expression vector was constructed for H.

Divergent Carry-Over Effects of Hypoxia during the Early Development of Abalone.

After being exposed to environmental stimuli during early developmental stages, some organisms may gain or weaken physiological regulating abilities, which would have long-lasting effects on their performance. Environmental hypoxia events can have significant effects on marine organisms, but for breeding programs and other practical applications, it is important to further explore the long-term physiological effects of early hypoxia exposure in economically significant species. In this study, the Pacific abalone was exposed to moderate hypoxia (∼4 mg/L) from zygote to trochophora, and the assessments of hypoxia tolerance were conducted on the grow-out stage.

Effects of one-year exposure to ocean acidification on two species of abalone.

Ocean acidification (OA) resulting from the absorption of excess atmospheric CO by the ocean threatens the survival of marine calcareous organisms, including mollusks. This study investigated the effects of OA on adults of two abalone species (Haliotis diversicolor, a subtropical species, and Haliotis discus hannai, a temperate species). Abalone were exposed to three pCO conditions for 1 year (ambient, ~ 880, and ~ 1600 μatm), and parameters, including mortality, physiology, immune system, biochemistry, and carry-over effects, were measured.