Summary of the best evidence for vocal rehabilitation training in adults with unilateral vocal fold paralysis.

Purpose: To search, evaluate, and summarize the relevant evidence of voice rehabilitation training in adults with unilateral vocal fold paralysis (UVFP).

Methods: Using the '6S' model, the database of UpToDate, BMJ Best Practice, domestic and foreign guidelines, and voice rehabilitation training for adults with UVFP were systematically searched. The search includes clinical decisions, guidelines, systematic reviews, randomized controlled trials, and expert consensus.

Explaining variations in the stability of the ecosystem of the largest fresh lake in China from 2013 to 2023.

Poyang Lake is the largest freshwater lake in China. In recent years, the stability of the lake's ecosystem has been challenged by climate change and human activities. It is important to study changes in the lake to understand how to maintain the health of the lake ecosystem.

The synthesis and bioactivity of apigenin derivatives.

Background: Apigenin, a naturally occurring compound with a flavone core structure, is known for its diverse bioactivities, including anti-inflammation, anti-toxicant, anti-cancer and so on. There has been significant interest in the medicinal chemistry community. To address these challenges, researchers have developed various derivatives of apigenin to address challenges such as poor water-solubility and low intestinal absorption, aiming to enhance the pharmacological activities and pharmacokinetic properties of this compound.

Study on the impact of digital economy on industrial collaborative agglomeration: Evidence from manufacturing and productive service industries.

In the digital era, digital economy has a far-reaching impact on the collaborative agglomeration of manufacturing and service industries. This research aims to examine the economic relationship between digital economy and industrial collaborative agglomeration. Based on a panel data set of 286 Chinese cities, this research employs Tobit model, moderating effect model, and mediating effect model to conduct data analysis.

Ginsenoside Compound K Reduces Psoriasis-related Inflammation by Activation of the Glucocorticoid Receptor in Keratinocytes.

Aim: To investigate the effects and mechanism of Ginsenoside Compound K (GCK) on psoriasis, focusing on the glucocorticoid receptor (GR) in keratinocytes.

Methods: An imiquimod (IMQ)-induced psoriasis-like dermatitis mouse model was generated to evaluate the anti-inflammatory effect of GCK. Hematoxylin and eosin (H&E) staining was used to assess skin pathological changes.

Ginsenoside compound K exerts anti-inflammatory effects through transcriptional activation and transcriptional inhibition of glucocorticoid receptor in rheumatoid arthritis fibroblast-like synoviocytes.

Ginsenoside compound K (GCK) has anti-inflammatory and immunoregulatory effects, and glucocorticoid receptor (GR) has been considered as its potential target. But the mechanism by which GCK exerts its anti-inflammatory effects after GR activation remains unclear. In this study, molecular docking, isothermal titration calorimetry, siRNA of GR and GRA458T mutation were used to confirm the anti-inflammatory mechanism of GCK targeting GR in fibroblast-like synoviocytes (FLS).

Polysorbate 80 as a possible allergenic component in cross-allergy to docetaxel and fosaprepitant: A literature review.

Objective: Patients had allergies to both fosaprepitant and docetaxel with similar signs and symptoms. To explore the possible causes of allergy and whether there is cross-allergy between fosaprepitant and docetaxel, we conducted a literature review according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.

Methods: A systematic search of the following databases was performed: Pubmed, Embase, Cochrane Library, CINAHL, Scopus, Web of Science and Taylor & Francis.

One-Pot Assembly of Dual-Site-Specific Antibody-Drug Conjugates via Glycan Remodeling and Affinity-Directed Traceless Conjugation.

The drug-to-antibody ratio (DAR) value and dual-drug combination greatly influence the therapeutic index of antibody-drug conjugates (ADCs). The reported approaches usually require multifunctional branched linkers, a combination of complicated technologies, or protein-protein ligation, which may incorporate multihydrophobic fragments or result in low coupling efficiency. Herein, we developed a facile and efficient one-pot method to assemble dual-site-specific ADCs with defined DARs at both the N-glycosylation site and K248 site, either with the same payloads or with two types of payloads.

Enhanced antibody-defucosylation capability of α-L-fucosidase by proximity-based protein fusion.

Up to date, the reported fucosidases generally show poor activities toward the IgG core-fucose, which limits the efficiency of ENGase-catalyzed glycoengineering process. However, EndoS or EndoS2 owns excellent activity and great selectivity towards the N-glycosylation of IgGs, and their non-catalytic domains are deduced to have specific interactions to IgG Fc domain that result in the great activity and selectivity. Herein, we constructed a series fusion protein of AlfC (an α-l-fucosidase from Lactobacillus casei BL23) with EndoS/S2 non-catalytic domain by replacing the catalytic GH (glycan hydrolase) domain of EndoS/S2 with the AlfC.

Hiding Payload Inside the IgG Fc Cavity Significantly Enhances the Therapeutic Index of Antibody-Drug Conjugates.

The inadequate understanding of the structure-activity relationship (SAR) of glycosite-specific antibody-drug conjugates (ADCs) hinders its design and development. Herein, we revealed the systemic SAR and structure-toxicity relationship (STR) of gsADCs by constructing 50 gsADC structures bearing three glycan subtypes and diverse linker-drug combinations. According to the results, extra hydrophilic linkers are indispensable for the intact glycan-based gsADCs to achieve better efficacy.

Evaluation of river longitudinal connectivity based on landscape pattern and its application in the middle and lower reaches of the Yellow River, China.

River connectivity plays an essential role in maintaining the health and stability of river basin ecosystems. It is of great significance to protect river ecosystems to clarify the effect of water conservancy project construction and operation on river hydrological connectivity. The longitudinal connectivity is affected by the landscape patterns of river, such as the convergence and dispersion of the mid-channel bars and the river areas.

F MRI-fluorescence imaging dual-modal cell tracking with partially fluorinated nanoemulsions.

As a noninvasive "hot-spot" imaging technology, fluorine-19 magnetic resonance imaging (F MRI) has been extensively used in cell tracking. However, the peculiar physicochemical properties of perfluorocarbons (PFCs), the most commonly used F MRI agents, sometimes cause low sensitivity, poor cell uptake, and misleading results. In this study, a partially fluorinated agent, perfluoro--butyl benzyl ether, was used to formulate a F MRI-fluorescence imaging (FLI) dual-modal nanoemulsion for cell tracking.

Flow and turbulence in unevenly obstructed channels with rigid and flexible vegetation.

The pattern of vegetation along the cross-section of macrophyte-dominated shallow waters is generally uneven, which affects water velocity and turbulence. This study examined the velocity and turbulence in the open channel with an uneven transverse distribution of vegetation in laboratory flume experiments. Two vegetation patterns were tested: emergent vegetation which covered part of the channel, and a symmetrical combination of submerged and emergent vegetation canopies along the lateral direction of the flume.

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB.

Peoniflorin-6'-O-benzene sulfonate (CP-25) inhibited the activity of GRK2 to exert anti-inflammatory and immunomodulatory effects. This study aimed to investigate the effect of CP-25 the intestinal epithelial barrier and the mechanism. CaCO-2 cell monolayer and dextran sulfate salt (DSS)-induced colitis mouse model was used to evaluate intestinal epithelial barrier function in vitro and in vivo, respectively.

Anticancer activity of oleanolic acid and its derivatives modified at A-ring and C-28 position.

Oleanolic acid (OA) is a five-ring triterpenoid compound, which is widely present in plants. Due to a wide range of pharmacological activities, oleanolic acid has attracted more and more attention. However, oleanolic acid is insoluble in water and has low bioavailability, which limits its clinical application.

A Traceless Site-Specific Conjugation on Native Antibodies Enables Efficient One-Step Payload Assembly.

Direct chemical modification of native antibodies in a site-specific manner remains a great challenge. Ligand-directed conjugation can achieve the selective modification of antibodies, but usually requires multiple extra steps for ligand release and cargo assembly. Herein, we report a novel, traceless strategy to enable the facile and efficient one-step synthesis of site-specific antibody-drug conjugates (ADCs) by harnessing a thioester-based acyl transfer reagent.

The nexuses between carbon emissions, agriculture production efficiency, research and development, and government effectiveness: evidence from major agriculture-producing countries.

Agriculture production efficiency and carbon emissions have become the challenge for the sustainable world. Therefore, this study explores the relationships between agriculture production and carbon emissions in major (seventeen) agriculture-producing countries over the time period of 1996-2018. Data envelopment analysis is applied to estimate the efficiency of agriculture sector production.

Health risk assessment of heavy metals in fish and accumulation patterns in food web in the upper Yangtze River, China.

Unlabelled: This study aims to concern the distribution of As, Cr, Cd, Hg, Cu, Zn, Pb and Fe in surface sediment, zoobenthos and fishes, and quantify the accumulative ecological risk and human health risk of metals in river ecological system based on the field investigation in the upper Yangtze River. The results revealed high ecological risk of As, Cd, Cu, Hg, Zn and Pb in sediment. As and Cd in fish presented potential human health risk of metals by assessing integrated target hazard quotient results based on average and maximum concentrations, respectively.

Ecological risk assessment of heavy metals in sediment in the upper reach of the Yangtze River.

Heavy metal pollution in China's Yangtze River basin has been of high concern. The concentrations of heavy metals (Cr, Cd, Hg, Cu, Zn, Pb, and As) in the sediment were investigated in the upper reaches of the river, China. Sediment quality guidelines (SQGs), an enrichment factor (EF), an index of geo-accumulation (I geo), and potential ecological risk were used to evaluate the extent of contamination from the heavy metal concentrations in the sediment.

Risk analysis of emergent water pollution accidents based on a Bayesian Network.

To guarantee the security of water quality in water transfer channels, especially in open channels, analysis of potential emergent pollution sources in the water transfer process is critical. It is also indispensable for forewarnings and protection from emergent pollution accidents. Bridges above open channels with large amounts of truck traffic are the main locations where emergent accidents could occur.