Publications by authors named "Cai-Yao Zhan"
Cytotechnology
March 2016
Article Synopsis
- The use of gene expression markers like EGFP has been important for selecting mammalian cell clones that produce high levels of recombinant proteins, but more cost-effective methods are still needed.
- A new method is introduced that co-expresses EGFP and a target protein linked by an internal ribosome entry site, enabling efficient clonal selection in standard 96-well plates.
- This technique was tested by expressing recombinant porcine β-defensin 1 in Marc 145 cells, showing its effectiveness as an alternative to traditional cell sorting methods for identifying high-producer clones.
