Risk factors analysis and prediction model construction for severe pneumonia in older adult patients.

Objective: Pneumonia is a common and serious infectious disease that affects the older adult population. Severe pneumonia can lead to high mortality and morbidity in this group. Therefore, it is important to identify the risk factors and develop a prediction model for severe pneumonia in older adult patients.

Suppression of circular RNA serum and glucocorticoid-induced kinase 1 elevates antioxidant molecules and angiogenesis in trophoblast cells to attenuate preeclampsia via microRNA-508-3p to target and restrain PUM homolog 1.

Aim: Preeclampsia (PE) is a pregnancy-specific syndrome characterized by hypertension and proteinuria. Recently, multiple circular RNAs (circRNAs) were considered latent clinical diagnostic markers or therapeutic targets. This study was to explore the impact of circRNA serum and glucocorticoid-induced kinase 1 (SGK1) on PE via influencing the microRNA (miR)-508-3p/PUM homolog 1 (PUM1) axis.

A Cadaveric Limb Analysis of the Posterior Tibial Musculotendinous Junction to determine the feasibility of Interosseous Membrane Tendon Transfer.

The transfer of the posterior tibial tendon through the interosseous membrane is potentially an effective treatment to correct the deformity of the foot and ankle. Our study aimed to evaluate the anatomical feasibility of anterior transfer of the posterior tibial tendon through the interosseous membrane route using the musculotendinous junction (MTJ). Eighteen adult cadavers were used.

CTHRC1 promotes growth, migration and invasion of trophoblasts via reciprocal Wnt/β-catenin regulation.

Preeclampsia (PE) is a pregnancy complication that is characterized by high blood pressure and is associated with high maternal and fetal morbidities. At a mechanistic level, PE is characterized by reduced invasion ability of trophoblasts. Collagen triple helix repeat containing-1 (CTHRC1) is a well-known tumor-promoting factor in several malignant tumors, but its role in trophoblasts remains unknown.

Identification, characterization and expression profiles of PSEN2 in the Chinese tree shrew ().

The gene encodes presenilin-2, a subunit of γ-secretase. Mutations in are not only related to Alzheimer's disease but are also involved in other diseases. The Chinese tree shrew () is a potential animal model for Alzheimer's disease, although little is known about its cDNA sequence, protein structure, and expression.

mRNA profiling reveals response regulators of decreased fungal keratitis symptoms in a tree shrew model.

Fungal keratitis is a corneal disease with a high blindness rate caused by pathogenic fungal infections. The pathogenesis of fungal keratitis and the immune response after fungal infection are still unclear. Notably, the pathological features of fungal keratitis in tree shrews are similar to those in humans.

CircRNAs in the tree shrew () brain during postnatal development and aging.

Circular RNAs (circRNAs) are a novel type of non-coding RNA expressed across different species and tissues. At present, little is known about the expression and function of circRNAs in the tree shrew brain. In this study, we used RNA-seq to identify 35,007 circRNAs in hippocampus and cerebellum samples from infant (aged 47-52 days), young (aged 15-18 months), and old (aged 78-86 months) tree shrews.

A Novel MEF2C Loss-of-Function Mutation Associated with Congenital Double Outlet Right Ventricle.

Congenital heart defect (CHD) represents the most prevalent birth defect, and accounts for substantial morbidity and mortality in humans. Aggregating evidence demonstrates the genetic basis for CHD. However, CHD is a heterogeneous disease, and the genetic determinants underlying CHD in most patients remain unknown.

A new nomogram score for predicting surgery-related pressure ulcers in cardiovascular surgical patients.

The aim of this study was to build a new nomogram score for predicting surgery-related pressure ulcers (SRPU) in cardiovascular surgical patients. We performed a prospective cohort study among consecutive patients with cardiovascular surgery between January 2015 and December 2015. Univariate and multivariate logistic regression was used to analyse the risk factors for SRPU.

A novel HAND2 loss-of-function mutation responsible for tetralogy of Fallot.

Congenital heart disease (CHD), the most common type of developmental abnormality, is associated with substantial morbidity and mortality in humans worldwide. The basic helix-loop-helix transcription factor, heart and neural crest derivatives expressed 2 (HAND2), has been demonstrated to be crucial for normal cardiovascular development in animal models. However, whether a genetically defective HAND2 contributes to congenital heart disease (CHD) in humans remains to be explored.

FOXP3 demethylation as a means of identifying quantitative defects in regulatory T cells in acute coronary syndrome.

Objective: The contribution of regulatory T cells (Tregs) to the pathogenesis of acute coronary syndrome (ACS) remains poorly understood. One core obstacle is the lack of Treg-specific markers. A highly conserved CpG enriched element in forkhead box P3 intron 1 (FOXP3 i l) is unmethylated only in Tregs, and measuring the unmethylation of FOXP3 i l can be used to identify the role of Tregs in clinical diseases.

[Cloning of full-length coding sequence of tree shrew CD3E and prediction of its molecular characteristics].

The use of tree shrews (Tupaia belangeri) in human disease studies demands essential research tools, in particular cellular markers and their monoclonal antibodies for immunological studies. Here we cloned the full-length cDNAs encoding CD3E from total RNA of the spleen, liver and peripheral blood of tree shrews and analyzed their structural characteristics in comparison with other mammals by Discovery Studio software. The results showed that the open reading frame sequence of tree shrew CD3E was 582 bp, encoding 194 amino acids.

[Characteristics of nitrite accumulation in a hydrogenotrophic denitrification system].

A mathematic model for nitrite accumulation in a hydrogenotrophic denitrification system running in a sequencing batch mode was developed and denitrification coefficient alpha was defined to describe the process of nitrite accumulation. The reduction rates of nitrate and nitrite and the nitrite accumulation before/after the nitrite addition were investigated using a sequencing batch reactor. The result showed that in Run 1 the reduction rates of nitrate and nitrite were 6.

Slack and Slick KNa channels are required for the depolarizing afterpotential of acutely isolated, medium diameter rat dorsal root ganglion neurons.

Aim: Na+-activated K+ (K(Na)) channels set and stabilize resting membrane potential in rat small dorsal root ganglion (DRG) neurons. However, whether K(Na) channels play the same role in other size DRG neurons is still elusive. The aim of this study is to identify the existence and potential physiological functions of K(Na) channels in medium diameter (25-35 microm) DRG neurons.

[Hydrogenotrophic denitrification for the removal of nitrate in drinking water].

Autotrophic denitrification with hydrogen as the electron donor for the removal of nitrate in drinking water was investigated by using a bench-scale sequencing batch reactor in an attached growth system. Gas permeable membrane was employed as the hydrogen diffuser to improve the hydrogen mass transfer and decrease the explosion risk. The results showed that nitrate could be removed efficiently with this technology.

DNA sequencing of a plasmid with virulence from marine fish pathogen Vibrio anguillarum.

DNA sequence of a plasmid pEIB1 associated with virulence from the marine fish pathogen Vibrio anguillarum was determined using the methods of restriction endonuclease digestion, subcloning, and primer walking. The whole length of obtained pEIB1 DNA sequence was 66 164 bp, and the overall G+C content of DNA sequence is 42.7%.