Microbiological contamination in donor corneas preserved for medium-term.

To evaluate the characteristics of microbiological contamination in donor corneas preserved for medium-term. A total of 82 donated corneas from June 1, 2014 to November 30, 2014 were retrospectively analyzed. The corneas were preserved in cornea chambers medium-term solution at 4-8 °C for keratoplasty.

Promotion of minTBP-1-PRGDN on the attachment, proliferation and collagen I synthesis of human keratocyte on titanium.

Aim: To investigate the influence of minTBP-1-PRGDN on the attachment, proliferation and collagen I synthesis of human keratocyte on titanium (Ti) surface.

Methods: The chimeric peptide RKLPDAPRGDN (minTBP-1-PRGDN) was synthesized by connecting RKLPDA (minTBP-1) to the N-terminal of PRGDN, the influence of minTBP-1-PRGDN on the attachment, proliferation and collagen I synthesis of human keratocyte on Ti surface were tested using PRGDN and minTBP-1as controls. The keratocytes attached to the surface of Ti were either stained with FITC-labeled phalloidin and viewed with fluorescence microscope or quantified with alamar Blue method.

[Analysis of anatomy features for patients with narrow anterior chamber angle and primary iridociliary body cysts].

Objective: To investigate the prevalence and anatomy features of iridociliary body cysts in patients with narrow anterior chamber angle.

Methods: Retrospective case series study. The prevalence and anatomy features of iridociliary body cysts in 223 patients (402 eyes) were analyzed retrospectively with ultrasound biomicroscopy (UBM).

[Analysis of multifocal electroretinogram first-order kernel P(1) wave in anisometropic amblyopia].

Objective: To investigate the features of the multifocal electroretinogram (mfERG) first-order kernel P(1) wave in anisometropic amblyopic eyes.

Methods: mfERG of fifteen anisometropic amblyopic eyes and their normal control eyes were recorded with ROLAND RETIscan visual evoked response system, the mean amplitude density and eclipse period of the P(1) wave were analyzed and compared between the amblyopic and normal eyes with t-test.

Results: The P(1) wave amplitude density of mfERG first-order kernel in amblyopic eyes (164.

[The investigation of tranilast on the proliferation and migration of human Tenon's capsule fibroblasts].

Objective: To investigate the effect of tranilast, N-(3,4-dimethoxycinnamoyl) anthramilic acid, on the proliferation and migration of human Tenon's capsule fibroblasts.

Methods: fibroblasts were cultured from Tenon's tissue of a glaucoma patient after trabeculectomy. The subcultured cells were incubated with DMEM medium containing different concentrations of tranilast for 72 h.