Publications by authors named "Cai-Lin Kong"

Carotenoids and their cleavage products (norisoprenoids) have excellent functional properties with diverse applications in foods, medicaments, cosmetics, . Carotenoids can be oxidatively cleaved through nonspecific reactions or by carotenoid cleavage oxygenases (CCOs), the product of which could further modify food flavor. This review provides comprehensive information on both carotenoid synthesis and cleavage processes with emphasis on enzyme characterization and biosynthetic pathway optimization.

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Article Synopsis
  • - The study explored the co-cultivation of *Pichia fermentans* Z9Y-3 and *S. cerevisiae* in synthetic grape must to enhance the production of fruity esters during fermentation.
  • - Findings indicated that mixed fermentation increased levels of various fruity esters (like ethyl acetate and medium chain fatty acid ethyl esters), with higher alcohols and fatty acids significantly influencing ester production more than enzymes.
  • - Supplementation with medium chain fatty acids during fermentation boosted fruity ester outputs by substantial percentages, but caution was advised as high ethyl acetate levels could lead to off-flavors.
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The dynamic pattern of volatiles during Pinot Noir winemaking in monsoon climate with yeast extracellular extract (EE) treatment was analyzed. EE from selected Pichia fermentans and Rhodotorula mucilaginosa strains, and almond β-glucosidase were added after 12-h alcohol fermentation, and the volatiles were determined every 24 h by GC-MS. After 6-month storage, wine aroma was evaluated instrumentally as well as by well-trained panelists.

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The chemical profiles and aroma contribution of terpene compounds in Meili grapes and wine were analyzed. Bound terpene compounds were extracted using methanol, purified using Amberlite XAD-2 resin, concentrated in methanol/ethyl acetate, and enzymatically hydrolyzed to release aglycones. Free terpene compounds were identified using solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS).

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