Effects of sacred music on the spiritual well-being of bereaved relatives: a randomized clinical trial.

OBJECTIVE To evaluate the effects of instrumental sacred music and sacred music with vocals on the spiritual well-being of bereaved relatives. METHOD This is a randomized clinical trial carried out with family members bereaving the death of loved ones to cancer. Participants were allocated into three groups: Group 1 (control), Group 2 (experimental using sacred music with vocals) or Group 3 (experimental using instrumental sacred music).

[Prospective study of cytomegalovirus infections in 211 children with kidney transplants].

A prospective serologic study was conducted to determine the incidence of primary Cytomegalovirus (CMV) infection after a renal transplant and to evaluate the consequences on the graft. Serologic tests using the indirect hemagglutination technique with CMV-sensitized erythrocytes were performed serially over 6 years in 370 children initially awaiting renal transplantation. 211 children received a graft during the study period.

Natural variation of poliovirus neutralization epitopes.

Poliovirus-neutralizing monoclonal antibodies were prepared against type 1, type 2, and type 3 wild laboratory (Mahoney, MEF1, and Saukett) and Sabin vaccine strains. Fifty-five poliovirus laboratory strains and field isolates were assayed by neutralization index test with a panel of homotypic monoclonal antibodies. A total of 27 monoclonal antibodies were used.

[Diphteric and tetanic antitoxins seric values in the first year of life, before and after immunization an horizontal survey in Mozambic (author's transl)].

Successive evaluations of diphtheritic (AD) and tetanic antitoxin have been made from birth to twelve months in infants receiving at 3, 4 and 5 months an immunization with antidiphtheritic, antitetanic and absorbed antimeasles vaccine. The decrease of AD of maternal origin is not, contrary to common opinion, regularly exponential. Factors influencing the elimination of AD from maternal origin are discussed.

Freeze-dried erythrocytes for an indirect hemagglutination test for detection of cytomegalovirus antibodies.

An indirect hemagglutination test with lyophilized, fixed, tanned, and cytomegalovirus (CMV)-sensitized sheep erythrocytes for the detection of CMV antibodies is reported. To avoid nonspecific hemagglutination, cells were fixed with glutaraldehyde or Formalin directly in whole blood. The lyophilized, CMV-sensitized erythrocytes obtained by this technique were stable up to 9 months at 37 degrees C and retained the same reactivity at fresh, CMV-sensitized cells.

Determination of type 1 poliovirus subtype classes with neutralizing monoclonal antibodies.

Hybridomas secreting neutralizing monoclonal antibodies (MA) against type 1 poliovirus were developed by fusion of mouse myeloma cells with splenocytes of immunized mice. Strain-specific MA selected for their capacity to neutralize wild (Mahoney) or attenuated (Sabin, LSc 2ab) poliovirus strains were used for identification of field isolates. A simple and reliable neutralization-index-assay was developed and used to test for intratypic serodifferentiation of poliovirus with the selected MA.

[Seroepidemiology of herpesvirus hominis and cytomegalovirus infections in the first 4 years of life (author's transl)].

Two hundred children and their mothers were examined when they were 10 months, 2 years and 4 years of age. Anti-cytomegalovirus, anti-Herpesvirus hominis and anti-rubella virus antibodies were titered. The evolution of antibody titers showed that primary infections with cytomegalovirus and Herpesvirus are especially frequent during the first months of life, and that mothers are the principal source of infection.

Evolution of serum immunoglobulin levels during the first year of life in Mozambique.

The serum levels of IgG, IgA and IgM as well as total proteins were followed from birth to one year of age in a sample of 44 infants. Values for a 'healthy' population are presented. The results of correlation analysis question current views on the independence of the different immunoglobulin classes.

Seroepidemiology of cytomegalovirus infections during the first years of life in urban communities.

Using indirect haemagglutination assay, combined with a collection of blood samples on blotting cards, seroepidemiological surveys of cytomegalovirus infections during early infancy have been done in different populations (French and immigrant) in urban areas. The comparison of CMV antibody status of mothers and their children at 10 months and at 2 years of age enables possible factors of viral transmission to be defined. During the first year of life, seropositive mothers were the only source of infection and they remained the main source during the second year.

Comparison of occurrence of antibodies to human cytomegalovirus as demonstrated by immunofluorescence and indirect hemagglutination techniques.

Indirect immunofluorescence and hemagglutination study of reactivity to human cytomegalovirus immediate early, early, and late antigens revealed that antibody responses to these antigens varies greatly and that hemagglutination is positive when sera contain only antibody to one or both early antigens.

[Epidemiology of cytomegalovirus infections (author's transl)].

Improvements of serological techniques allow seroepidemiologic surveys of cytomegalovirus infections. Intrauterine contamination is not unfrequent (1%) but pathological consequences of these infections in the fetus had only been observed after primary infections of the mother. Infections in the first days of life are frequent.

[Evolution of cytomegalovirus antibodies of maternal origin and acquired, throughout the first year of life (author's transl)].

Cytomegalovirus antibodies have been determined by indirect hemagglutination technique in sera taken during the first year of life in two infant groups : Bantu infants in Mozambique, French infants in Alsace. IgM specific antibodies have been detected in serum fractions obtained by density gradients. The evolutions of maternal antibodies and of antibodies resulting from a primary CMV infection have been documented.

A comparison of the evolution of transmitted IgG immunoglobulin and diphtheria antitoxin titers in the newborn.

The evolution of the titers of transmitted IgG and diphtheria antitoxin was studied in 20 neonates during the first 2 months of life (6 serum samples for each). A rapid fall of the passive IgG titer was observed during the first weeks: as a rule the higher the initial titer the greater the fall. After the 15th day the rate of decline slows down.

[Indirect hemagglutination test for detection of antibodies to cytomegalovirus in blood collected on blotting paper (author's transl)].

Indirect hemagglutination test for detection of antibodies to cytomegalovirus is highly sensitive and reproducible, if employed in well-defined conditions. Standardization of the various factors involved is necessary as well as their reciprocal equilibrium : sheep erythrocytes, antigen, dilution of tanin, buffers quality. The hemagglutination test can be performed on small volumes such as blood collection on blotting paper (PKU).

[Blood serum levels of immunoglobulins among African children: Seasonal variations and ethnic factors (a study done in Lourenco-Marques among a group of 11-year olds) (author's transl)].

The blood titers of IgG, IgA and IgM and total proteins were determined in October 1971 (dry season) and again in April 1972 (wet season) among girls and boys belonging to 4 ethnic groups: White, Bantu, Indian, Mixed. Parasitic studies were also done. These studies indicate a rise of IgM among the girls and of IgA in both sexes from October 1971 to April 1972.