Circular RNA as a source of neoantigens for cancer vaccines.

Background: The effectiveness of somatic neoantigen-based immunotherapy is often hindered by the limited number of mutations in tumors with low to moderate mutation burden. Focusing on microsatellite-stable colorectal cancer (CRC), this study investigates the potential of tumor-associated circular RNAs (circRNAs) as an alternative source of neoepitopes in CRC.

Methods: Tumor-associated circRNAs in CRC were identified using the MiOncoCirc database and ribo-depletion RNA sequencing of paired clinical normal and tumor samples.

The complete chloroplast genome sequence of .

In this study, we first presented the complete chloroplast genome of by using Illumina Novaseq sequencing. Its complete chloroplast genome is 156,596 bp in length, containing a large single copy region of 88,477 bp and a small single copy region of 20,379 bp separated by a pair of inverted repeat regions of 23,870 bp. The chloroplast genome contains 112 unique genes, including 78 protein-coding genes, 30 tRNA, and four rRNA genes.

Characterization and complete genome sequences of two novel variants of the family Closteroviridae from Chinese kiwifruit.

Two distinct closterovirus-like genome sequences (termed AdV-1 v1 and v2) were identified in Actinidia chinensis var. deliciosa 'Miliang-1' that had no disease symptoms using high-throughput sequencing. Using overlapping reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends, the genomic sequences of AdV-1 v1 and v2 were confirmed as 17,646 and 18,578 nucleotides in length, respectively.

Characterisation of the complete chloroplast genome of Swingle and phylogenetic relationships.

In this study, we reported the complete chloroplast genome of Swingle using the HiSeq-4000 sequencing. The chloroplast genome size is 160,229 bp, which consists of a large single-copy region (87,774 bp), a small single-copy region (18,721 bp), and a pair of IR regions (26,867 bp). The chloroplast genome contains 114 unique genes, including 80 protein-coding genes, 30 tRNAs, and 4 rRNAs.

Characterization of the complete chloroplast genome of Bolus in China and phylogenetic relationships.

Gerbera ( Bolus) is one of the most popular ornamental flowers and one of the top five cut flowers worldwide. In this study, we presented the complete chloroplast genome of it using BGISEQ-500 sequencing. Its chloroplast genome is 151,898 bp in size, containing a large single copy region (83,518 bp) and a small single copy region (18,244 bp), and a pair of IR regions (25,068 bp).

Characterization of the complete chloroplast genome of Sanming wild banana () and phylogenetic relationships.

Sanming wild banana () is a cold resistant wild banana found in Fujian province of China. In this study, we characterized its complete chloroplast genome using BGISEQ-500 sequencing. The chloroplast genome is 171,815 bp in size, containing a pair of IR regions (35,142 bp), a large single copy region (89,995 bp), and a small single copy region (11,464 bp).

Characterization of the complete chloroplast genome of the Hongkong kumquat ( Swingle).

Hongkong qumquat ( Swingle) is a wild citrus species native to China. In this study, we firstly reporteded its complete chloroplast genome using BGISEQ-500 sequencing. The chloroplast genome is 160,145 bp in size, containing a large single copy region (87,467 bp), a small single copy region (18,730 bp), and a pair of IR regions (26,974 bp).

Self-adjustment and disintegration threshold of Langmuir solitons in inhomogeneous plasmas.

Dynamics of Langmuir solitons in the presence of a background density gradient is investigated numerically, including cases with steep gradients to the extent the solitons can disintegrate. The disintegration threshold is explained by regarding the electric field part of the soliton as a point mass moving along the self-generated potential well corresponding to the density cavity. On the other hand, it is demonstrated that the Langmuir solitons are robust when the density gradient is below the threshold.

Manipulation of novel nano-prodrug composed of organic pigment-based hybrid network and its optical uses.

Here we developed the first case of pyropheophorbide-a-loaded PEGylated-hybrid carbon nanohorns (CNH-Pyro) to study tumor targeting therapy. During incubation with living cells, CNH-Pyro exhibited very intense red emissions. The intracellular imaging results were carried out by flow cytometry based on four different kinds of cell lines (including three adherent cell lines and one suspension cell line).

Electron and Ion Heating Characteristics during Magnetic Reconnection in the MAST Spherical Tokamak.

Electron and ion heating characteristics during merging reconnection start-up on the MAST spherical tokamak have been revealed in detail using a 130 channel yttrium aluminum garnet (YAG) and a 300 channel Ruby-Thomson scattering system and a new 32 chord ion Doppler tomography diagnostic. Detailed 2D profile measurements of electron and ion temperature together with electron density have been achieved for the first time and it is found that electron temperature forms a highly localized hot spot at the X point and ion temperature globally increases downstream. For the push merging experiment when the guide field is more than 3 times the reconnecting field, a thick layer of a closed flux surface form by the reconnected field sustains the temperature profile for longer than the electron and ion energy relaxation time ~4-10 ms, both characteristic profiles finally forming a triple peak structure at the X point and downstream.

Electron temperature and density probe for small aeronomy satellites.

A compact and low power consumption instrument for measuring the electron density and temperature in the ionosphere has been developed by modifying the previously developed Electron Temperature Probe (ETP). A circuit block which controls frequency of the sinusoidal signal is added to the ETP so that the instrument can measure both T(e) in low frequency mode and N(e) in high frequency mode from the floating potential shift of the electrode. The floating potential shift shows a minimum at the upper hybrid resonance frequency (f(UHR)).

Comparative Transcriptome and iTRAQ Proteome Analyses of Citrus Root Responses to Candidatus Liberibacter asiaticus Infection.

Root samples of 'Sanhu' red tangerine trees infected with and without Candidatus Liberibacter asiaticus (CLas) were collected at 50 days post inoculation and subjected to RNA-sequencing and isobaric tags for relative and absolute quantification (iTRAQ) to profile the differentially expressed genes (DEGs) and proteins (DEPs), respectively. Quantitative real-time PCR was subsequently used to confirm the expression of 16 selected DEGs. Results showed that a total of 3956 genes and 78 proteins were differentially regulated by HLB-infection.

Relationship between wave-like auroral arcs and Pi2 disturbances in plasma sheet prior to substorm onset.

Wave-like substorm arc features in the aurora and Pi2 magnetic disturbances observed in the near-Earth plasma sheet are frequently, and sometimes simultaneously, observed around the substorm onset time. We perform statistical analyses of the THEMIS ASI auroral observations that show wave-like bright spot structure along the arc prior to substorm onset. The azimuthal mode number values of the wave-like substorm arcs are found to be in the range of ~100-240 and decrease with increasing geomagnetic latitude of the substorm auroral arc location.

Multichannel microwave interferometer with an antenna switching system for electron density measurement in a laboratory plasma experiment.

This study presents a simple and powerful technique for multichannel measurements of the density profile in laboratory plasmas by microwave interferometry. This technique uses electromechanical microwave switches to temporally switch the connection between multiple receiver antennas and one phase-detection circuit. Using this method, the phase information detected at different positions is rearranged into a time series that can be acquired from a minimum number of data acquisition channels (e.

Electrode contamination effects of retarding potential analyzer.

The electrode contamination in electrostatic analyzers such as Langmuir probes and retarding potential analyzers (RPA) is a serious problem for space measurements. The contamination layer acts as extra capacitance and resistance and leads to distortion in the measured I-V curve, which leads to erroneous measurement results. There are two main effects of the contamination layer: one is the impedance effect and the other is the charge attachment and accumulation due to the capacitance.

Rice protein exerts a hypocholesterolemic effect through regulating cholesterol metabolism-related gene expression and enzyme activity in adult rats fed a cholesterol-enriched diet.

The major aim of this study is to elucidate the hypocholesterolemic mechanism exerted by rice protein (RP) in adult rats under cholesterol-enriched dietary condition. Compared with casein, the cholesterol levels in plasma and the liver were significantly reduced by RP, accompanying significant inhibition of cholesterol absorption. RP increased the activity and mRNA level of cholesterol 7α-hydroxylase, whereas acyl-CoA:cholesterol acyltransferase activity and gene expression were significantly depressed with consumption of RP.

Means to remove electrode contamination effect of Langmuir probe measurement in space.

Precaution to remove the serious effect of electrode contamination in Langmuir probe experiments has not been taken in many space measurements because the effect is either not understood or ignored. We stress here that one should pay extra attention to the electrode contamination effect to get accurate and reliable plasma measurements so that the long time effort for sounding rocket/satellite missions does not end in vain or becomes less fruitful. In this paper, we describe two main features of voltage-current characteristic curves associated with the contaminated Langmuir probe, which are predicted from the equivalent circuit model, which we proposed in 1970's.

Causes of inpatient death for patients with warfare-related limb trauma and logistic regression analysis of the risk factors.

Objectives: To explore the causes and risk factors of inpatient death for patients with warfare-related limb trauma.

Methods: A retrospective study involving 339 patients with warfare-related limb trauma who were admitted to our hospital from 1998 to 2002 was conducted. Autopsy was performed for 15 cases who died in order to investigate the cause of death.

Experimental identification of electromagnetically induced transparency in magnetized plasma.

We report the first experimental identification of the new wave branch at electron cyclotron frequency produced by the injection of a frequency-matched intense pump wave in magnetized plasma [A. G. Litvak and M.

Ion and electron heating characteristics of magnetic reconnection in a two flux loop merging experiment.

Characteristics of the high-power reconnection heating were measured for the first time directly by two-dimensional measurements of ion and electron temperatures. While electrons are heated mainly inside the current sheet by the Ohmic heating power, ions are heated mainly by fast shock or viscosity damping of the reconnection outflow in the two downstream areas. The magnetic reconnection converts the energy of reconnecting magnetic field B(p) mostly to the ion thermal energy, indicating that the reconnection heating energy is proportional to B(p)(2).

Lithium plasma emitter for collisionless magnetized plasma experiment.

This paper presents a newly developed lithium plasma emitter, which can provide quiescent and low-temperature collisionless conditions for magnetized plasma experiments. This plasma emitter generates thermal emissions of lithium ions and electrons to produce a lithium plasma. Lithium type beta-eucryptite and lanthanum-hexaboride (LaB(6)) powders were mixed and directly heated with a tungsten heater to synthesize ion and electron emissions.

[The causes of death and risk factors in patients of war wound and trauma of extremities by logistic regression model].

Objective: To explore the causes of death and risk factors in patients of war wound and trauma of extremities.

Methods: This retrospective study involved 352 patients of war wound and trauma of extremities admitted to 303rd Hospital of People's Liberation Army during the period between 1968 to 2002. All the data were reviewed and the causes of death of 15 patients were analyzed by autopsy, and a computer's logistic regression model analysis was performed to approach the risk factors of death.

[Serial thin cross-sectional anatomy and computerized three-dimensional reconstruction of metacarpal fascial spaces of hand].

Objective: To build three-dimensional (3-D) visible model for surgical treatment of infection of fascial spaces of hand.

Methods: Serial thin cross-sections (0.2 mm) of hand were made by cryomicrotome, and the thin cross-sections of metacarpal parts were observed.

New interpretation of alpha-particle-driven instabilities in deuterium-tritium experiments on the Tokamak Fusion Test Reactor.

The original description of alpha particle driven instabilities in the Tokamak Fusion Test Reactor in terms of toroidal Alfvén eigenmodes (TAEs) remained inconsistent with three fundamental characteristics of the observations: (i) the variation of the mode frequency with toroidal mode number, (ii) the chirping of the mode frequency for a given toroidal mode number, and (iii) the antiballooning density perturbation of the modes. It is now shown that these characteristics can be explained by observing that cylindrical-like modes can exist in the weak magnetic shear region of the plasma that then make a transition to TAEs as the central safety factor decreases in time.

[Digital technology of X-ray radiography].

This paper introduces to you some methods to radlige the digital radiogzaphy, and there respectiae princiales and featuus.