A large number of American troops fighting in Afghanistan and Iraq have received wounds in their upper extremities leading to significant nerve damage and loss of strength. These injuries impair their ability to perform day-to-day tasks such as lifting a cup of coffee or opening a door. Although the cause of some injuries in service-people is often unique to their employment, civilian employees in other industries are also plagued with similar physical damage due to other kinds of injuries.
View Article and Find Full Text PDFABSTRACT Transmission electron microscopy was used to study the penetration and infection of pansy roots by Thielaviopsis basicola. Events observed in 7- to 10-day-old roots produced on moist filter paper differed slightly from those in roots from 4-week-old plants washed free of potting media prior to inoculation. By 3 h postinoculation (PI), epidermal cells of roots produced on filter paper exhibited aggregated cytoplasm and papilla formation in response to germ tube tips.
View Article and Find Full Text PDFABSTRACT Observations were made of the ultrastructure of infection and colonization of leaves of a susceptible maize inbred by Colletotrichum graminicola and by a C. graminicola pathogenicity mutant. The mutant causes no symptoms on either maize leaves or stalks.
View Article and Find Full Text PDFThis article reports on the use of high pressure freezing followed by freeze substitution (HPF/FS) to study ultrastructural details of host-pathogen interactions in fungal diseases of plants. The specific host-pathogen systems discussed here include a powdery mildew infection of poinsettia and rust infections of daylily and Indian strawberry. The three pathogens considered here all attack the leaves of their hosts and produce specialized hyphal branches known as haustoria that invade individual host cells without killing them.
View Article and Find Full Text PDFPlant Dis
September 2003
An unusual and undescribed foliar blight of tall fescue was observed in a home lawn and in turf grass research plots near Griffin, GA in May and June, 2000 and 2001. Isolation from lesions yielded mycelium of a basidiomycete with hyphal characteristics (binucleate cells, absence of clamp connections) associated with Laetisaria and Limonomyces spp. Isolates from blighted tall fescue and an isolate of Limonomyces roseipellis formed a clade distinct from isolates of Laetisaria fuciformis based on ribosomal DNA sequences.
View Article and Find Full Text PDFBoth conventional chemical fixation and high pressure freezing followed by freeze substitution (HPF/FS) were used to prepare zoosporangia of the oomycete Albugo ipomoeae-panduratae inside infected host leaves for study with transmission electron microscopy. Both fixations gave good preservation of ultrastructural details and data from the two sample types were highly complementary. However, HPF/FS gave better overall specimen contrast and superior preservation of microtubules, basal bodies and curved vacuoles closely associated with basal bodies.
View Article and Find Full Text PDFTransmission electron microscopy was used to examine details of the host-pathogen interface in daylily leaf cells infected by the rust fungus Puccinia hemerocallidis. Samples were prepared for study by high-pressure freezing followed by freeze substitution. The outstanding preservation of ultrastructural details afforded by this fixation protocol greatly facilitated the study of this host-pathogen interface.
View Article and Find Full Text PDFEntomosporium mespili appears to be a hemibiotroph on infected Photinia leaves. This fungal pathogen produced distinctive haustoria in living host cells in young lesions. Each haustorium possessed a long slender neck with a single septum and an enlarged distal body that contained a single nucleus.
View Article and Find Full Text PDFAspergillus nidulans is an ascomycetous fungus that reproduces asexually by forming multicellular conidiophores and uninucleate spores called conidia. Loss of function mutations in the abacus A (abaA) regulatory locus result in formation of aberrant conidiophores that fail to produce conidia. Wild-type conidiophores form two tiers of sterigmata.
View Article and Find Full Text PDFConidium (asexual spore) differentiation in wild-type and the wet-white (wetA) mutant of Aspergillus nidulans was compared in intact chains of successively older conidia. Carbohydrate cytochemistry helped define three stages (Stages I, II, and III) of wild-type conidium maturation on the basis of changes in the ultrastructure and composition of the conidium wall. Conidia of the wetA6 mutant strain formed normally but failed to mature during Stages II and III.
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