[Adenylate cyclase activation and its effects on intracellular cAMP in infected KB cells during trypsin-induced infectious Sendaï virus production (author's transl)].

KB cells infected by Sendaï virus can produce infectious virus if they are trypsinated twice over 24 h. Adenylate cyclase activity in infected KB cells is higher and more strongly activated by trypsin than that of control cells, but intracellular concentration of cAMP is the same, except during a short time after trypsinations, especially after the second trypsination which causes infectious virus production. During this short time, intracellular cAMP is slightly higher in infected cells.

[Adenylate cyclase activation in KB cells infected by Sendaï virus (author's transl)].

Adenylate cyclase activity is increased in KB cells infected by a little dose of Sendaï virus. The apparent Km for ATP substrate is decreased from 0.7 to 0.

[alpha-Amylase activity in lysosomes of Aspergillus oryzae (author's transl)].

The alpha-amylase of mycelial cells of Aspergillus oryzae exists in a particular form in 8000 g pellet. The lysosomal localization of acid phosphatase is confirmed by electron microscopy. The purification of lysosomes by discontinuous gradient of sucrose in D2O shows that alpha-amylase activity is bound to these particles.

[Unmasking effect of trypsin on Sendai virions obtained in KB cell cultures (author's transl)].

The infectivity of virus grown in KB cell cultures can be directly activated by trypsin. Thus the fit conditions for seeding are effected from subculture to subculture if the cell inoculum is pretreated by trypsin.

[The humoral immune response in recent pulmonary tuberculosis (author's transl)].

The average serum concentration of immunoglobulins, alpha-1-antitrypsin, haptoglobin and alpha-2-macroglobulin, calculated in 45 adults with pulmonary tuberculosis increased in a variable way for each of the six parameters studied. Tuberculosis does not induce new correlations between the parameters but modifies the intensity of existing links.

[Inhibition of DNA biosynthesis in human lymphocytes by alpha 1-antitrypsin].

Incorporation of tritiated thymidine into human lymphocytes from tonsils is markedly inhibited by purified alpha1-antitrypsin-preparations. This inhibitory effect is observed in lymphocytes stimulated by a mitogen factor (phytohemagglutinin) as in non stimulated lymphocytes.

[Optimal conditions of alpha-amylase production by Aspergillus oryzae in liquid media].

The alpha-amylase secretion in a mineral culture medium containing starch and glucose follow the lysis of mycelium. This lysis seems to result from the hydrolysing action of dextranase and levulanase on cell wall. Cell lysis and amylase secretion are greatly enhanced by pH elevation of culture medium (optimal pH 8,8).

[Production of interferon and alpha 2-macroglobulin involvement in immune response during human viral hepatitis A (author's transl)].

In viral hepatitis A, we could distinguish between monophasic and polyphasic forms. Monophasic and polyphasic hepatitis A induce the production of plasma interferon. Interferon level, elevated as early as the first days following the appearance of clinical signs, decreases and reaches a minimum value on the seventh day.

[Study of various serum proteins in lung cancer. Immunoglobulins A, G, M, haptoglobin, alpha-1-antitrypsin, alpha-2-macroglobulin].

A statistical study of the plasma concentration of immunoglobulin, alpha-1-antitrypsin, haptoglobin, and alpha-2-macroglobulin in 153 patients with primary carcinoma of the lung showed a strong increase in alpha-1-antitrypsin, haptoglobin and A and C immunoglobulins, whilst alpha-2-macroglobulin increases very moderately, and IgM does not vary. The relationships between variables are also modified, thus there appears a correlation between IgA and IgG to the detriment of the IgG/IgM relationship. Furthermore the coefficient of the correlation IgG/alpha-2-macroblobulin is lower in cancer patients.

[Enzymatic characteristics of the guanylate cyclase of KB cells: their change as a function of the development of the cultures].

We have localized 71% of the guanylate cyclase activity in the (G X 105,000) supernatent fraction of broken KB cells. The reaction follows Michaelis-Menten kinetics, the apparent Km for GTP is 0,5 mM, as long as GTP is lower than a limited concentration, then activity is inhibited. The ion Mn++ is an absolutely required activator, it does not change enzyme-substrate affinity.

[Enzymatic characteristics of the catalytic subunit of the protein kinase complex of human lymphocytes].

In earlier reports we have shown the existence in human lymphocytes homogenate, of a cyclic-AMP dependent protein-kinase activity. We demonstrate by affinity chromatography that two subunits display respectively cyclic-AMP binding and phosphorylating properties. Divalent cations such as Ca++, Mg++ or Mn++ are required for enzymatic activity.

[Identification of cAMP dependant protein kinases in human lymphocytes].

Chromatographic purification by "DEAE" cellulose resolves the cAMP binding proteins in human lymphocytes into three parts. In presence of Mg++ each one possesses cAMP dependent protein-kinase activity, one of them showing allosteric characteristics.