Wound bed preparation: theory to practice.

This article provides an overview of and rationale for wound bed preparation. It seeks to increase nurses' knowledge and understanding of the roles of the main cells and growth factors, involved in the wound healing process, and also to highlight how theory relates to practice.

The elements of wound assessment.

Accurate wound assessment is essential to the appropriate and realistic planning of goals and interventions for patients with wounds. However, the assessment process has a number of components that must be systematically considered.

Translational regulation of the plant S-adenosylmethionine decarboxylase.

It is becoming apparent that control of protein synthesis by metabolites is more common than previously thought. Much of that control is exerted at the level of initiation of mRNA translation, orchestrated by upstream open reading frames (uORFs) and RNA secondary structure. S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in polyamine biosynthesis and both mammalian and plant AdoMetDCs are translationally regulated by uORFs in response to polyamine levels by distinct mechanisms.

Topical negative pressure therapy.

Topical negative pressure therapy (TNP) has been available for clinical use in wound management since the late 1990s. It has been shown to be of particular benefit for the treatment of chronic nonhealing wounds and has also recently been positively linked to wound bed preparation.

The challenge of wound exudate.

The past two decades have seen many advances in tissue viability. Our knowledge of the processes involved in wound healing has increased vastly, and as a result many products and techniques have been developed to speed it up. However, patients with wounds, and the professionals who care for them, still experience a number of challenges.

Wound-bed management: key principles for practice.

Most chronic wounds have become stuck in the late inflammatory phase of wound healing. This can be distressing and frustrating for patients who may have lived with such a wound for many months. The key to the concept of wound-bed management is to prepare the wound so that modern methods of promoting healing can then be applied.

Tibial interface wear in retrieved total knee components and correlations with modular insert motion.

Background: Wear occurring at the interface between the polyethylene insert and metal baseplate of a modular tibial component has become an increasingly common finding at the time of revision total knee arthroplasty. Although this so-called backside wear on retrieved polyethylene inserts has been evaluated in prior studies, wear on retrieved metal baseplates has not been described, to our knowledge. The purposes of the present study were to characterize backside wear on retrieved polyethylene inserts and on the mating surfaces of their corresponding baseplates and to investigate if there is a relationship between backside wear and relative motion of the modular elements.

Production and detection of cold antihydrogen atoms.

A theoretical underpinning of the standard model of fundamental particles and interactions is CPT invariance, which requires that the laws of physics be invariant under the combined discrete operations of charge conjugation, parity and time reversal. Antimatter, the existence of which was predicted by Dirac, can be used to test the CPT theorem-experimental investigations involving comparisons of particles with antiparticles are numerous. Cold atoms and anti-atoms, such as hydrogen and antihydrogen, could form the basis of a new precise test, as CPT invariance implies that they must have the same spectrum.

Alphavirus replicon particles as candidate HIV vaccines.

Replicon particles based on Venezuelan equine encephalitis virus (VEE) contain a self-replicating RNA encoding the VEE replicase proteins and expressing a gene of interest in place of the viral structural protein genes. Structural proteins for packaging of replicon RNA into VEE replicon particles (VRPs) are expressed from separate helper RNAs. Aspects of the biology of VEE that are exploited in VRP vaccines include 1) expression of very high levels of immunogen, 2) expression of immunizing proteins in cells in the draining lymph node, and 3) the ability to induce mucosal immunity from a parental inoculation.

Pain and tissue trauma during dressing change.

Background: Preventing patient pain and tissue trauma to the wound and surrounding skin are key considerations for nurses at the time of dressing changes. Mark Collier and Helen Hollinworth present the preliminary findings from a recent survey among nurses with an interest in wound care on the selection of dressing types.

Conclusion: Education of student and post-registration nurses about the properties of dressing products, with an understanding of the potential detrimental effects as a result of pain and trauma, is needed to help promote quality wound care.

Management of patients with fungating wounds.

In the absence of much literature about fungating wounds, Mark Collier presents a thought-provoking case history and describes the principles underpinning the optimal care of patients with fungating (malignant) wounds.

Nurses' views about pain and trauma at dressing changes: results of a national survey.

Research findings and professional concerns that patients still experience pain and tissue trauma at dressing changes led specialist nurses to develop a postal questionnaire to elicit the views of 1000 nurses with an interest in wound management. The questionnaire asked practitioners to identify the primary considerations underpinning their approach to pain and tissue trauma at dressing changes, the strategies they adopted, and the factors which determined their treatment choices. The higher than anticipated response rate (37%) illustrates the importance nurses attach to this aspect of practice.

Oestrogen protects FKBP12.6 null mice from cardiac hypertrophy.

FK506 binding proteins 12 and 12.6 (FKBP12 and FKBP12.6) are intracellular receptors for the immunosuppressant drug FK506 (ref.

Metal content of dandelion (Taraxacum officinale) leaves in relation to soil contamination and airborne particulate matter.

The global distribution of the common dandelion (Taraxacum officinale Weber, sensu lato; Asteraceae), along with its ability to tolerate a wide range of environmental conditions, make this 'species' a particularly attractive candidate to evaluate for its value as a biological monitor of environmental metal contamination. To examine the metal content of dandelion leaves in relation to environmental metal levels, the concentrations of eight metals (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) were analyzed in leaf and soil samples collected at 29 sites in the mid-western United States differentially impacted by pollution. Sites were chosen primarily to cover a range of annual mean 24-h airborne particulate matter < or = 10 microm (hereafter, PM10) exposure, with PM10 levels varying from those found in isolated rural areas to levels typical of the most industrialized urban locations in the mid-western United States.

In vivo deterioration of tibial baseplate locking mechanisms in contemporary modular total knee components.

Background: The results of recent studies documenting the backside wear of polyethylene inserts retrieved from total knee implants call into question the stability of the locking mechanisms of modular tibial components. Wear of the metal tibial baseplate suggests that the capture mechanisms of some modular fixed-bearing tibial components do not adequately restrict in vivo motion of the insert. The purposes of this study were (1) to present a method for evaluating locking-mechanism stability and (2) to investigate the stability of modular tibial components after an interval in vivo.

Health risk assessment of dioxin emissions from municipal waste incinerators: the Neerlandquarter (Wilrijk, Belgium).

Two municipal waste incinerators in the vicinity of a residential area close to the city of Antwerp caused concern to local habitants. Risk assessment was performed combining chemical, toxicological measurements and model calculations. As the first step in risk assessment an inventory was made of historic emissions from both incinerators with emphasis on dioxins.

Cytogenetic effects of 900 MHz (GSM) microwaves on human lymphocytes.

The cytogenetic effects of 900 MHz radiofrequency fields were investigated with the chromosome aberration and sister chromatid exchange frequency methods. Three different modes of exposure (continuous, pseudo-random and dummy burst) were studied for different power outputs (0, 2, 8, 15, 25, 50 W). The specific absorption rates varied between 0 and 10 W/kg.

Phase I dose-escalation and pharmacokinetic study of a novel folate analogue AG2034.

The novel folate analogue AG2034, which was designed as an inhibitor of GARFT (glycinamide ribonucleotide formyltransferase), was evaluated in this phase I study under the auspices of The Cancer Research Campaign, UK. AG2034 blocks de novo purine synthesis through inhibition of GARFT. A total of 28 patients with histologically proven intractable cancers were enrolled.

Cytogenetic effects of 50 Hz magnetic fields of different magnetic flux densities.

Cytogenetic investigations were performed in human peripheral blood lymphocytes following exposure to 50 Hz magnetic fields alone or in combination with the chemical mutagen mitomycin C or with X-rays. It was found that magnetic fields up to 2500 microT did not significantly influence the chromosome aberration and sister chromatid exchange frequency. Also, the combined treatments failed to indicate the presence of any synergistic, potentiating, or antagonistic effect between the ELF magnetic fields and the mutagens.

Cytogenetic investigations on microwaves emitted by a 455.7 MHz car phone.

The chromosome aberration or sister chromatid exchange frequency was determined in 455.7 MHz microwave-exposed human lymphocytes and in lymphocytes that were subsequently exposed to MMC or X-rays. The exposure was performed by placing the cells at 5 cm from the antenna of a car phone.