Serum Biomarkers of Pulmonary Damage and Risk for Progression of Rheumatoid Arthritis-Associated Interstitial Lung Disease.

Objective: To investigate baseline and change of pulmonary damage biomarkers (serum Krebs von den Lungen 6 [KL-6], human surfactant protein D [hSP-D], and matrix metalloproteinase 7 [MMP-7]) with rheumatoid arthritis-associated interstitial lung disease (RA-ILD) progression.

Methods: In the Korean Rheumatoid Arthritis Interstitial Lung Disease (KORAIL) cohort, a prospective cohort, we enrolled patients with RA and ILD confirmed by chest computed tomography imaging and followed annually. ILD progression was defined as worsening in physiological and radiological domains of the 2022 American Thoracic Society, European Respiratory Society, Japanese Respiratory Society, and Latin American Thoracic Society guideline for progressive pulmonary fibrosis (PPF).

enhancement of CD8+ T cell activity using functionalized hydrogel encapsulating tonsil-derived lymphatic endothelial cells.

This study investigates a method for programming immune cells using a biomaterial-based system, providing an alternative to traditional cell manipulation techniques. It addresses the limitations of engineered adoptive T cell therapies, such as T cell exhaustion, by introducing a gelatin-hyaluronic acid (GH-GMA) hydrogel system. We characterized tonsil mesenchymal stem cells (TMSCs), lymphatic endothelial cells (T-LECs), stimulated T-CD8 T cells (STCs), and GH-GMA biomaterials.

Seasonal Dynamics of Ticks and Tick-Borne Pathogens in Republic of Korea.

Tick-borne diseases are a public health problem and a significant burden on the livestock industry. The seasonal abundance of ticks and tick-borne pathogens strongly correlates with the prevalence of these diseases. To investigate the seasonal variation in ticks and tick-borne pathogens, ticks were collected from Gangwon State, Korea, and the tick-borne pathogens , , , and were examined.

Comparison of Biocompatibility of 3D-Printed Ceramic and Titanium in Micropig Ankle Hemiarthroplasty.

Background: Ankle arthritis is a common degenerative disease that progresses as cartilage damage in the lower tibia and upper talus progresses, resulting in loss of joint function. In addition to typical arthritis, there is also structural bone loss in the talus due to diseases such as talar avascular necrosis. Total talus replacement surgery is the procedure of choice in end-stage ankle arthritis and consists of a tibial, talar component and an insert.

Transcriptomic Changes in Human Tonsil-Derived Mesenchymal Stem Cells Across Culture Passages.

Background/objectives: Tonsil-derived mesenchymal stem cells (TMSCs) are in the limelight in regenerative medicine due to their high proliferation and differentiation potential. It is important to conduct studies to determine the optimal conditions for achieving the maximum yield while maintaining the optimal differentiation capacity of TMSCs.

Methods: This study explores the impact of serial subculture on TMSCs by analyzing gene expression at passages 2, 4, 6, and 8.

Radical Mastectomy Efficiently Improves Long-Term Clinical Outcomes in Dogs with Malignant Mammary Tumors.

The incidence of mammary gland tumors in dogs is a significant issue, characterized by notable rates of malignancy, recurrence, and disease burden. However, the lack of comprehensive information and correlated studies has presented challenges in establishing an effective surgical treatment strategy. In this study, a cohort of 95 dogs diagnosed with mammary tumors underwent evaluation using various surgical methods, medications, ovariohysterectomy, and assessments of margin completeness to analyze clinical outcomes.

Uniportal Full Endoscopic 270° Decompression for Thoracic 1-2 Hard Disc Herniation With Ossification of the Ligamentum Flavum.

This article aims to demonstrate the uniportal full endoscopic surgery for treating complex anterior and posterior spinal pathology at the T1-2 level, offering a invasive, accessible, stable, and versatile approach to challenging anatomical situations. Uniportal full endoscopic surgery is one of the most minimally invasive spinal surgeries, utilizing slim, elongated, and compact instruments that provide access to lesions from any angle and distance. This characteristic makes the technique especially suitable for hard, such as the T1-2 level, where traditional approaches may be limited or difficult.

Effects of genetic mutations on left ventricular myocardial mechanics and fibrosis patterns in hypertrophic cardiomyopathy.

Myocyte disarray and fibrosis are underlying pathologies of hypertrophic cardiomyopathy (HCM) caused by genetic mutations. However, the extent of their contributions has not been extensively evaluated. In this study, we investigated the effects of genetic mutations on myofiber function and fibrosis patterns in HCM.

Perspective of 3D culture in medicine: transforming disease research and therapeutic applications.

Unlabelled: 3D cell culture is gaining momentum in medicine due to its ability to mimic real tissues () and provide more accurate biological data compared to traditional methods. This review explores the current state of 3D cell culture in medicine and discusses future directions, including the need for standardization and simpler protocols to facilitate wider use in research.

Purpose: 3D cell culture develops life sciences by mimicking the natural cellular environment.

Lentivirus-based production of human chimeric antigen receptor macrophages from peripheral blood.

Although chimeric antigen receptor (CAR) T cell therapy has shown remarkable efficacy against leukemic cells, it still has critical limitations. CAR macrophage has been regarded as a potential alternative to CAR T cells. However, due to the difficulties in gene transduction into macrophages, the production of primary human CAR macrophages from peripheral blood mononuclear cells (PBMC) using lentivirus is highly challenging.

Correction: Comparative efficacy of 0.1% and 0.15% Sodium Hyaluronate on lipid layer and meibomian glands following cataract surgery: A randomized prospective study.

[This corrects the article DOI: 10.1371/journal.pone.

Prospective validation and revision of predictive models for post-ERCP pancreatitis: focus on procedure-related factors and a novel risk stratification approach.

Introduction: Post-endoscopic retrograde cholangiopancreatography pancreatitis (PEP) is the most common serious adverse event after endoscopic retrograde cholangiopancreatography (ERCP). Although retrospective models to predict PEP have shown promise, their real-world applicability remains uncertain. Thus, we used prospectively derived cohort data to validate current prediction models.

Frequent complications of deficient patellae after component removal in 1.5-stage exchange arthroplasty for periprosthetic joint infection.

Purpose: To compare the complication rates between deficient patellae (remnant patellae after component removal) and non-resurfaced patellae after 1.5-stage exchange arthroplasty for periprosthetic joint infection and identify risk factors for complications of deficient patellae.

Methods: Complications in 76 deficient patellae (group D) and 41 non-resurfaced patellae (group N), with a minimum follow-up of 2 years after 1.

Clinical and Imaging Features of a Focal Intrahepatic Biliary Stricture Visualized Only as Duct Dilatation.

Purpose: We assessed the proportion of patients with a focal intrahepatic stricture (FIHS) that was a precursor lesion or malignancy and visualized only as a duct dilatation.

Materials And Methods: This retrospective study assessed patients who underwent surgery or biopsy for an FIHS on CT or MRI between January 2010 and March 2022. The number and proportion of non-precursor benign lesions, precursors, and malignancies were calculated.

First record of Monalonion velezangeli (Hemiptera: Miridae) affecting avocado and cherimoya (Annona cherimola) as new hosts in Ecuador.

Monalonion velezangeli is a polyphagous hemipteran reported as one of the main pests of economic importance in avocado and coffee crops. This is the first report of M. velezangeli affecting avocado (Persea americana (Mill)) in Ecuador and the first report of cherimoya (Annona cherimola) as a new host plant.

Reconstructed three-dimensional structure of gas-foamed polycaprolactone/cellulose nanofibrous scaffold for biomedical applications.

One of the unavoidable issues with the bio-scaffolding process is the collapse of the visually appealing external three-dimensional (3D) sponge-like structure and the internal porous and multilayered morphology of a gas-foamed nanofibrous scaffold. Herein, a gas-foamed polycaprolactone/cellulose (g-PCL/CL) nanofibers scaffold is first prepared by electrospinning PCL/cellulose acetate, followed by deacetylation and then Sodium borohydride-assisted gas-foaming technique. The deformed 3D architecture of g-PCL/CL nanofiber is finally reconstructed by mixing it with chitosan (CS) solution and molding.

Interpersonal Factors and Resilience Mediate the Association Between Work-Related Stress in Response to a Viral Epidemic and Depression Among Healthcare Workers in the COVID-19 Pandemic.

Objective: This study aims to explore the association between work-related stress of healthcare workers in response to the coronavirus disease-2019 (COVID-19) pandemic and their depressive symptoms. Additionally, it focuses on the impact of insomnia symptoms, interpersonal factors, and resilience on depressive symptoms.

Methods: The results of an anonymous survey of 329 healthcare workers were analyzed, including the Stress and Anxiety to Viral Epidemics-3 items, Insomnia Severity Index, Interpersonal Needs Questionnaire, Connor-Davidson Resilience Scale-2 items, and Patient Health Questionnaire-9 items.

Sex-Specific Association of Low Muscle Mass with Depression Status in Asymptomatic Adults: A Population-Based Study.

Background: The objective of this study was to examine the correlation between low muscle mass (LMM) and depression, with a specific focus on identifying the sex-specific relationship between LMM and depression in a large sample.

Methods: This population-based cross-sectional study involved 292,922 community-dwelling adults from 2012 to 2019. Measurements were taken using the Center for Epidemiological Studies Depression (CESD) scale and body composition analyses.