The autonomic innervation of rat jugular vein.

The autonomic innervation of rat jugular vein was studied using glyoxylic acid fluorescence and acetylcholinesterase histochemical methods. The rat jugular vein is provided with both adrenergic and cholinergic nerve fibers organized in plexuses located at the adventitial-medial border. The existence of these nerve plexuses does not seem to support biochemical findings that suggests a lack of innervation in the rat jugular vein and which propose this blood vessel as a model for the analysis of drug-smooth muscle cell interaction without the interference of neuronal uptake mechanisms.

Cholinergic nerves in the parietal pleura.

Using the cholinesterase histochemical technique we investigated the innervation pattern of parietal pleura both in normal and chemically sympathectomized rats. AChE containing nerve fiber-like structures were observed within the parietal pleura. The apex, costal, and mediastinal surfaces of the pleura are poorly innervated, while the diaphragmatic pleura shows the richest innervation.

Cholinergic nerves in the human liver.

The cholinergic innervation of the human liver was studied. Slices (150--200 micrometer thick) of human liver and of the greater hepatic blood vessels (hepatic artery and vein, portal vein) were incubated in a solution of 6-hydroxydopamine (6-HDA) in order to obtain a selective degeneration of adrenergic nerves. Controls were prepared from samples incubated with buffer alone.

Acetylcholinesterase containing nerve fibres in coronary circulation.

The cholinergic innervation of coronary arteries and veins has been studied in the human. Structures resembling cholinergic nerve fibres are localised at the level of the extraparenchymal branches of the coronary arteries, organised in the adventitial plexus. Neither the coronary veins nor the intraparenchymal blood vessels are provided with a cholinergic innervation.

Acetylcholinesterase containing nerve fibers in guinea pig ovary.

The distribution of acetylcholine esterase (AChE) in the ovary of normal as well as of sympathectomized guinea pigs was studied. In normal animals AChE-positive nerve fibres were found organized in a perivascular plexus. Some nerve fibres reach the corpus luteum and the follicular wall.

Cholinergic nerves in the mesentery.

The cholinergic innervation of extravascular portions of rat mesentery was studied in normal as well as 6-hydroxydopamine (7 OHDA) sympathectomized animals. We observed the presence of a cholinergic innervation unaltered by 6 OHDA treatment in extra-vascular portions of mesentery. Cholinergic nerve fibers coming from paravascular nerves forms loop]-like structures that innervate the mesentery proper.

Beta adrenergic receptors in the parathyroid glands.

The location of beta-adrenoceptors in human parathyroid gland was studied using an immunohistochemical method. Frozen sections of human parathyroid glands, taken from surgical samples, were treated with (-)-alprenolol, washed and exposed to (-)-alprenolol antibodies conjugated with fluorescent dyes. The (-)-alprenolol was bound to the parathyroid principal cells and to the main blood vessels.

Effect of bombesin on glucose transport system in biomembranes.

Bombesin is able to stimulate the glucose transport system supported by a contractile glucose binding protein (GBP) in biomembranes. The increase of glucose transport indicates that bombesin affects GBP.

[Anterior communicating artery: morphologic and structural aspects].

The gross anatomy of the anterior communicating artery has been studied in 40 adult brains removed at autopsy. We have observed 6 different anatomicals variant of the artery. A histological study shows that the wall of the artery is constituted with three layers: intima, media and adventitia.

Acetylcholinesterase-containing nerve fibers in the dura mater of guinea pig, mouse, and rat.

The cholinergic innervation of the dura mater in the guinea pig, mouse and rat has been investigated. The dura mater is provided with a cholinergic innervation. After short incubation times (4--6 hours) we found acetylcholinesterase (AChE)-containing nerve fibers in close relationship with the main meningeal blood vessels.

The cholinergic innervation of the aorta.

The cholinergic innervation of the aorta has been studied in some mammalian species. Cholinergic nerve fibers (CNF) are organized in the aorta of the rabbit, kid, lamb, and guinea pig in 2 plexuses. The plexuses of CNF consist of a superficial plexus localized in the adventitial layer and a deep one localized in the adventitial-medial transitional zone.

Myosin-like protein in the human parathyroid glands: an immunohistochemical study.

The immunofluorescent staining of the human parathyroid glands shows that the principal cells bind antimyosin-like antibodies. The presence of a myosin-like protein in the principal cells of the human parathyroid glands is presumably in rapport with parathormone secretion.

Tests to localize free proteolytic enzymes in vivo by 14C-cysteine-aprotinin.

Aprotinin, a polyvalent protease inhibitor from bovine organs, has been labelled with 14C-cysteine in 6 M urea to produce a radioactive conjugate, without effect on the inhibitor activity. 138x10(3) dpm of radioactive product, containing 5 mg of protein (20.000 kal.

Cholinergic nerves in blood vessels of the female reproductive system.

Cholinergic innervation of the blood vessels of the female reproductive system was investigated in human by the cholinesterase method. The following results were obtained: 1. The uterine and vaginal arteries are provided with a rich cholinergic innervation.

Glucose-6-phosphate dehydrogenase in kidneys of lead-poisoned rats and adrenalectomized rats.

Glucose-6-phosphate dehydrogenase (G6PDH) activity, assayed biochemically, was significantly increased in kidney homogenates of lead-poisoned rats when compared with controls. Histochemically, G6PDH activity was greatly increased both in the distal tubules and the macula densa, but showed no significant changes in the proximal tubules. Biochemical assay of G6PDH in kidney homogenates of adrenalectomized rats was three times that in control animals.

[Adrenergic receptors in coronary arteries (author's transl)].

The relations between adrenergic nerve fibers and related receptors in the wall of the coronary arteries have been studied in man. On sections of these vessels morphological observations (H.E.

Myosin-like microfilaments in the human normal and pathological testis.

The immunofluorescent staining of the human normal testis shows that both peritubular and tubular cells bind antimyosin-like antibodies. The same cells are provided with a large amount of microfilaments within the cytoplasm. The thin microfilaments observed by electron microscopy in various cells of the human testis likely correspond to the sites of immunofluorescent staining.

The contractile nature of the tubular-like cells of the parietal layer of the Bowman's capsule in male mice. An immunochemical and ultrastructural study.

The tubule-like cells (TLC) of the parietal layer of the Bowman's capsule of the renal corpuscle in normal male mice are provided with bundles of thin microfilaments (50-70 A). Moreover, the ultrastructural observations demonstrated that similar thin filaments (50-70 A) are located in the flattened cells of the parietal layer of the Bowman's capsule and in the tubular cells. All the above cells bind antimyosin-like antibodies.