The significance of complement fixation test in clinical diagnosis of toxoplasmosis.

Background: The complement fixation test (CFT) is the basic method in the diagnosis of toxoplasmosis. Despite its standard and reproducible results, it is rarely used in routine diagnosis of toxoplasmosis where the detection of IgG by means of EIA tests is widely used.

Objective: The study is focused on the verification of CFT value within the spectrum of specific classes of immunoglobulins IgG, IgM, IgA antibodies and avidity of IgG antibodies, as well as on the clinical diagnosis of toxoplasmosis.

[Laboratory diagnosis of toxoplasmosis].

Toxoplasmosis is a world-wide spread parasitosis. The disease potentially highly affects two groups of patients: foetus and immunosuppressed patients. The determination of diagnosis and therapy on the basis of a single serum examination is very important; possible on the basis of a single serum sample.

[Toxoplasma gondii].

The authors offered a general view on Toxoplasma gondii from the historical, taxonomic, biological and epidemiological point of view. They also studied the problems of pathogenesis, pathology, clinics, diagnosis, as well as therapy and prevention. (Tab.

[Morphology and diagnosis of the oral protozoans Trichomonas tenax and Entamoeba gingivalis using the Giemsa-Romanovsky stain].

In the microscopic diagnosis of Trichomonas tenax and Entamoeba gingivalis is the technically and time not demanding native preparation of a culture, in which both protozoans can be detected according to their typical motility, determining. In the permanent preparation of the culture stained according to Giemsa-Romanovsky, which has also documentary character, are all of the characteristic cell organelles stainable, enabling so their detection without their typical motility. Staining according to Giemsa-Romanovsky is technically simple and not time consuming, not very laborious, low cost and the coloration is permanent, that means optimal for the diagnostic of oral protozoans in permanent preparations.

[Pathologic karyotypes in autopsy material].

In 1986-1989 the authors were concerned with the cultivation of necroptic material. Material was collected from five indication groups outlined in advance. A total of 231 specimens of necroptic material were cultivated, incl.

[Postmortem chromosome analysis and its significance].

Cultivation of necroptic material taken by pathologist or obstetrician according to determined indicative groups was performed during the years 1986-1990. Cytogenetical evaluation was feasible in 157 cultivated samples from the total of 252. There were found 32 pathological karyotypes among them.

[Occurrence of the protozoa, Entamoeba gingivalis and Trichomonas tenax in the mouths of children and adolescents with hyperplastic gingivitis caused by phenytoin].

The oral protozoa Entamoeba gingivalis and Trichomonas tenax do not occur in small children and are rarely found in older ones. In adolescents their occurrence rate keeps increasing with age. They parasitize in an oral cavity changed by inflammation, yet also in a healthy mouth.

[The role of Toxoplasma gondii in the etiology of chronic tonsillitis in children].

Toxoplasma gondii is known to have an affinity to lymphatic tissue. A series of 506 children with chronic tonsillitis were examined serologically for toxoplasmosis by means of CBR, IIR and ELISA. In 7.

[The role of Toxoplasma gondii in the etiology of chronic tonsillitis in children].

Toxoplasma gondii is know to have an affinity to lymphatic tissue. The authors studied whether after infection of the child's body T. gondii does or does not determine the chronic course of tonsillitis.

[Morphology and diagnosis of Entamoeba gingivalis and Trichomonas tenax and their occurrence in children and adolescents].

Specimens obtained from 176 eight- to nineteen-year-old subjects were cultured for oral protozoa. Of the given series, 25 subjects (14.2%) had in their mouth only E.

[Cytogenetic examination of amniotic fluid specimens in the 2d trimester of pregnancy (personal experience 1981-1989)].

The authors analyze a group of pregnant women examined at the Chair of Biology and Parasitology of the Medical Faculty J. A. Komensky's University between 1981 and the end of August 1989.

[Experience with postmortem chromosome analysis].

Over the years 1986-1988 necroptic material, collected according to 5 established indication groups, was cultured. A total of 202 samples of necroptic material cultured and 122 of these samples were analyzed cytogenetically. Seventeen pathologic karyotypes were diagnosed in the material, namely 7 cases of Down's syndrome, 2 cases of Klinefelter's syndrome, 2 cases of D/D translocation, 1 case of Turner's syndrome, 1 case of gonosomal mosaicism, and 1 case of Patau's syndrome.

[Ethico-philosophic and socio-legal aspects of AIDS].

The authors analyze ethico-philosophical and socio-legal problems of patients with AIDS in their relation to people they are in frequent contact with, i.e. family, relatives, sexual partners, as well as medical and paramedical staff.

[Toxoplasma antibodies in children in Slovakia with potential toxoplasmosis].

Over the years 1984-1987, 829 children from the Slovak Socialist Republic, aged from 0 to 15 years, with suspect toxoplasmosis were examined for the presence of toxoplasma antibodies by the serodiagnostic methods RVK, NIR, and ELISA. The percentage of toxoplasma antibodies yielded by the individual methods was as follows: RVK--39.5%; NIR--74.