Publications by authors named "C. Bladier"

Human brain lectin (HBL), a beta-galactoside specific soluble lectin, was purified by affinity chromatography. An alkylated derivative of this lectin was also prepared. Both native and modified molecules were conserved at -20 degrees C in the presence or absence of beta-mercaptoethanol, a reducing agent which was described to maintain the lectin activity in vitro or in the presence of beta-mercaptoethanol and lactose.

View Article and Find Full Text PDF

Desialylated erythrocytes, when perfused in mice liver in situ, were shown to be sequestered. This sequestration was inhibited by dihydrocytochalasin B and a neoglycoprotein: N-Acetylgalactosaminyl-albumin. Our results confirm that, under physiological conditions, the recognition of desialylated erythrocytes appear to be related to the presence of N-Acetylgalactosamine or galactose receptor on the surface of Küpffer cells.

View Article and Find Full Text PDF

Rabbit erythrocytes were separated in vitro according to their age in vivo by centrifugation. The content of sialic acid and of neutral hexoses is significantly reduced on the old erythrocyte ghosts when compared to young ones. The content of fucose and hexosamines of young and old erythrocyte ghosts is similar.

View Article and Find Full Text PDF