Arthroscopic Repair of Subscapularis Tendon Using a Percutaneous Continuous Sewing Machine-Like Suture Technique Through a Single Working Portal.

Tearing of the subscapularis tendon is a common shoulder injury that typically requires arthroscopic repair. The suture-passing device is a standard tool for repairing the subscapularis tendon. However, it poses the risk of device breakage and may cause additional damage to the tendon.

Minipatellar Tunnels for Transosseous Fixation of Medial Patellofemoral Ligament Graft Using High-strength Suture.

Patellar dislocation is a common knee injury, with concomitant pathoanatomical risk factors that synergistically interact and predispose to patellofemoral instability. Medial patellofemoral ligament (MPFL) reconstruction has demonstrated significant potential in the re-establishment of MPFL anatomic and biological function, with low patellar redislocation rates. Although many techniques for MPFL reconstruction have been developed, challenges such as patella fractures and high costs persist.

Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches.

Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation.

Chitosan-based composite featuring dual cross-linking networks for the removal of aqueous Cr(VI).

To avoid the environmental detriment caused by Cr(VI) waste, this study constructs a dual cross-linking network structure using sodium alginate (SA) and polyvinyl alcohol (PVA). Chitosan (CS) is further introduced through electrostatic attraction and hydrogen bonding and SA/PVA/CS (SPC) composite with porous structure is successfully prepared for the removal of Cr(VI) from wastewater. Batch adsorption experiments show that SPC has excellent adsorption capacity and practical usability with a broad pH applicability range.

Distally based sural flaps for soft tissue defects following traumatic osteomyelitis of lower leg and foot.

This retrospective study aimed to determine the incidence and risk factors for osteomyelitis recurrence and present our experiences in treating traumatic osteomyelitis in the lower leg and foot. We retrospectively reviewed data from 174 patients with distally based sural flaps for treating traumatic osteomyelitis with soft tissue defects in the lower leg and foot from November 2003 to February 2021. Possible risk factors for osteomyelitis recurrence were compared between the osteomyelitis control and recurrence groups.

Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for CD8 T cell exhaustion and anti-PD-1 resistance.

Resistance to PD-1 blockade in onco-immunotherapy greatly limits its clinical application. T cell immunoglobulin and mucin domain containing-3 (Tim-3), a promising immune checkpoint target, is cleaved by ADAM10/17 to produce its soluble form (sTim-3) in humans, potentially becoming involved in anti-PD-1 resistance. Herein, serum sTim-3 upregulation was observed in non-small cell lung cancer (NSCLC) and various digestive tumors.

The significance of CD4+ and CD8+ T lymphocyte infiltration in esophageal squamous cell carcinoma.

Purpose: To investigate the relationship between the abundance of CD4+ and CD8+ T cells in the tumor microenvironment and the prognosis of patients with esophageal squamous cell carcinoma, and to analyze their correlation and explore its clinical value.

Materials And Methods: In total, we enrolled 120 cases of esophageal squamous cell carcinoma diagnosed. The abundance of CD4+ and CD8+ T lymphocytes in the tissue specimens of esophageal cancer was examined by immunohistochemistry.

First Observation of a Three-Resonance Structure in e^{+}e^{-}→Nonopen Charm Hadrons.

We report the measurement of the inclusive cross sections for e^{+}e^{-}→nOCH (where nOCH denotes non-open charm hadrons) with improved precision at center-of-mass (c.m.) energies from 3.

Vegetation redistribution is predicted to intensify soil organic carbon loss under future climate changes on the Tibetan Plateau.

Vegetation redistribution may bring unexpected climate-soil carbon cycling in terrestrial biomes. However, whether and how vegetation redistribution alters the soil carbon pool under climate change is still poorly understood on the Tibetan Plateau. Here, we applied the G-Range model to simulate the cover of herbs, shrubs and trees, net primary productivity (NPP) and soil organic carbon density (SOCD) at the depth of 60 cm on Tibetan Plateau for the individual years 2020 and 2060, using climate projection for Representative Concentration Pathways (RCP) 4.

IL-1β induced down-regulation of miR-146a-5p promoted pyroptosis and apoptosis of corneal epithelial cell in dry eye disease through targeting STAT3.

Aim: To elaborate the underlying mechanisms by which IL-1β promote progression of Dry eye disease(DED) through effect on pyroptosis and apoptosis of corneal epithelial cells(CECs).

Methods: 400 mOsM solutions were used to establish the DED model (hCECs- DED). RT-qPCR was performed to measure IL-1β mRNA and miR-146a-5p in CECs.

Long-term stable and efficient degradation of ornidazole with minimized by-product formation by a biological sulfidogenic process based on elemental sulfur.

Conventional biological treatment processes cannot efficiently and completely degrade nitroimidazole antibiotics, due to the formation of highly antibacterial and carcinogenic nitroreduction by-products. This study investigated the removal of a typical nitroimidazole antibiotic (ornidazole) during wastewater treatment by a biological sulfidogenic process based on elemental sulfur (S-BSP). Efficient and stable ornidazole degradation and organic carbon mineralization were simultaneously achieved by the S-BSP in a 798-day bench-scale trial.

A study of generative large language model for medical research and healthcare.

There are enormous enthusiasm and concerns in applying large language models (LLMs) to healthcare. Yet current assumptions are based on general-purpose LLMs such as ChatGPT, which are not developed for medical use. This study develops a generative clinical LLM, GatorTronGPT, using 277 billion words of text including (1) 82 billion words of clinical text from 126 clinical departments and approximately 2 million patients at the University of Florida Health and (2) 195 billion words of diverse general English text.

Spatial distribution and driving factors of soil organic carbon in the Northeast China Plain: Insights from latest monitoring data.

Soil organic carbon (SOC) is a critical component of soil fertility and plays a crucial role in the global carbon cycle. Despite the widespread reports of a decrease in SOC content and stock in the Northeast China Plain in recent decades, the current status and driving factors of its content and distribution are unclear. In this study, the surface soil (0-20 cm) SOC content data of 1920 sampling points within the Northeast China Plain covering an area of 2.

NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing.

The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and the vast dynamic range of the plasma proteome. Here we address these challenges with NUcleic acid Linked Immuno-Sandwich Assay (NULISA™), which improves the sensitivity of traditional proximity ligation assays by ~10,000-fold to attomolar level, by suppressing assay background via a dual capture and release mechanism built into oligonucleotide-conjugated antibodies. Highly multiplexed quantification of both low- and high-abundance proteins spanning a wide dynamic range is achieved by attenuating signals from abundant targets with unconjugated antibodies and next-generation sequencing of barcoded reporter DNA.

Advanced time-lagged effects of drought on global vegetation growth and its social risk in the 21st century.

Extensive studies have demonstrated the restricting effect of past and present drought conditions on vegetation growth over the past three decades. However, the underlying mechanism of the impact of prior drought on vegetation growth - along with the magnitude of its impact over the rest of the 21st century - remains uncertain. Herein, we examined the evolution and characteristics of global vegetation growth and drought for both baseline (1982-2014) and future (2015-2100) periods under four representative pathways using the gross primary productivity (GPP) and the Standardized Precipitation Evapotranspiration Index from the CMIP6.

Lactulose vs Polyethylene Glycol for Bowel Preparation: A Single-Center, Prospective, Randomized Controlled Study Based on BMI.

Introduction: Colonoscopy is currently considered as one of the principal techniques to diagnose the colorectal diseases. Admittedly, qualified bowel preparation before colonoscopy is a premise for high-quality examination. Lower quality bowel preparation might seriously impede visualization of the intestinal mucosa, resulting in missed and misdiagnosed intestinal lesions.

Detection of Intracerebral Hemorrhage Using Low-Field, Portable Magnetic Resonance Imaging in Patients With Stroke.

Background: Neuroimaging is essential for detecting spontaneous, nontraumatic intracerebral hemorrhage (ICH). Recent data suggest ICH can be characterized using low-field magnetic resonance imaging (MRI). Our primary objective was to investigate the sensitivity and specificity of ICH on a 0.

Mesoporous Iron Family Element (Fe, Co, Ni) Molybdenum Disulfide/Carbon Nanohybrids for High-Performance Supercapacitors.

As the demand for fuel continues to increase, the development of energy devices with excellent performance is crucial. Supercapacitors (SCs) are attracting attention for their advantages of high specific energy and a long cycle life. At present, the development of high-performance electrode materials is the main point for research and development of SCs.

Development of a Selective and High Affinity Radioligand, [H]VU6013720, for the M Muscarinic Receptor.

M muscarinic receptors are highly expressed in the striatum and cortex, brain regions that are involved in diseases such as Parkinson's disease, schizophrenia, and dystonia. Despite potential therapeutic advantages of specifically targeting the M receptor, it has been historically challenging to develop highly selective ligands, resulting in undesired off-target activity at other members of the muscarinic receptor family. Recently, we have reported first-in-class, potent, and selective M receptor antagonists.

Cerebral Oximetry Monitoring in Extremely Preterm Infants.

Background: The use of cerebral oximetry monitoring in the care of extremely preterm infants is increasing. However, evidence that its use improves clinical outcomes is lacking.

Methods: In this randomized, phase 3 trial conducted at 70 sites in 17 countries, we assigned extremely preterm infants (gestational age, <28 weeks), within 6 hours after birth, to receive treatment guided by cerebral oximetry monitoring for the first 72 hours after birth or to receive usual care.

Neoantigen-targeted CD8 T cell responses with PD-1 blockade therapy.

Neoantigens are peptides derived from non-synonymous mutations presented by human leukocyte antigens (HLAs), which are recognized by antitumour T cells. The large HLA allele diversity and limiting clinical samples have restricted the study of the landscape of neoantigen-targeted T cell responses in patients over their treatment course. Here we applied recently developed technologies to capture neoantigen-specific T cells from blood and tumours from patients with metastatic melanoma with or without response to anti-programmed death receptor 1 (PD-1) immunotherapy.

Venetoclax treatment in patients with cancer has limited impact on circulating T and NK cells.

Venetoclax is an effective treatment for certain blood cancers, such as chronic lymphocytic leukemia (CLL) and acute myeloid leukemia (AML). However, most patients relapse while on venetoclax and further treatment options are limited. Combining venetoclax with immunotherapies is an attractive approach; however, a detailed understanding of how venetoclax treatment impacts normal immune cells in patients is lacking.