Microspectrometer-Enabled Real-Time Concentration Monitoring in the Microfluidic Protein Enrichment Chip.

This study presents a novel microspectrometer-integrated microfluidic system for real-time protein concentration monitoring. The device employs electrokinetic principles for efficient protein preconcentration in a PDMS and Nafion film channel. Using FITC-labeled BSA as a model protein, the system demonstrated a linear correlation between protein concentration and absorbance at 491 nm.

New Library-Based Methods for Nontargeted Compound Identification by GC-EI-MS.

While gas chromatography mass spectrometry (GC-MS) has long been used to identify compounds in complex mixtures, this process is often subjective and time-consuming and leaves a large fraction of seemingly good-quality spectra unidentified. In this work, we describe a set of new mass spectral library-based methods to assist compound identification in complex mixtures. These methods employ mass spectral uniqueness and compound ubiquity of library entries alongside noise reduction and automated comparison of retention indices to library compounds.

NSE protein detection in a microfluidic channel integrated an electrochemical biosensor.

This study proposed a microfluidic chip for the detection and quantification of NSE proteins, aimed at developing a rapid point-of-care testing system for early lung cancer diagnosis. The proposed chip structure integrated an electrochemical biosensor within a straight PDMS microchannel, enabling a significant reduction in sample volume. Additionally, a method was developed to deposit silver and silver chloride layers onto the reference electrode.

Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer-Modified Magnetic Beads and Permalloy Micromagnets.

This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used with aptamer-modified magnetic beads (MBs) of various sizes to successfully separate lung cancer cells from a mixture of other cells. The overall separation efficiency was evaluated based on the ratios of cells in the different outlets and inlets of the chip.