Publications by authors named "C W Bohrer"
Most bacteria lack membrane-enclosed organelles and rely on macromolecular scaffolds at different subcellular locations to recruit proteins for specific functions. Here, we demonstrate that the optogenetic CRY2-CIB1 system from Arabidopsis thaliana can be used to rapidly direct proteins to different subcellular locations with varying efficiencies in live Escherichia coli cells, including the nucleoid, the cell pole, the membrane, and the midcell division plane. Such light-induced re-localization can be used to rapidly inhibit cytokinesis in actively dividing E.
View Article and Find Full Text PDFEnhancers are central for the regulation of metazoan transcription but have proven difficult to study, primarily due to a myriad of interdependent variables shaping their activity. Consequently, synthetic biology has emerged as the main approach for dissecting mechanisms of enhancer function. We start by reviewing simple but highly parallel reporter assays, which have been successful in quantifying the complexity of the activator/coactivator mechanisms at enhancers.
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- Discontinuous transcription is a key aspect of gene regulation, but the detailed mechanisms behind transcriptional bursting remain unclear.
- By using mathematical modeling on single-cell RNA sequencing data, researchers investigated the dynamics of transcriptional bursting and identified specific regulators like MED26, MYC, cohesin, and BRD4.
- MED26 notably affects the frequency of bursting and has the most significant impact on the gene regulatory network, showing that stages beyond the initial transcription start are crucial for integrating gene networks in individual cells.
Article Synopsis
- High-throughput imaging (HTI) generates large and complex datasets, but existing commercial software does not fully allow for customization or integration of new algorithms in image analysis.
- Open-source platforms face limitations in integrating new analysis modules, making it challenging to adopt advanced techniques.
- HiTIPS, a new open-source software, addresses these issues by offering user-friendly capabilities for automated analysis in cell biology, and supports the integration of new modules for enhanced image processing.
CCAT1 lncRNA is chromatin-retained and post-transcriptionally spliced.
Histochem Cell Biol
July 2024
Super-enhancers are unique gene expression regulators widely involved in cancer development. Spread over large DNA segments, they tend to be found next to oncogenes. The super-enhancer c-MYC locus forms long-range chromatin looping with nearby genes, which brings the enhancer and the genes into proximity, to promote gene activation.
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