Publications by authors named "C W Asbury"
Chromosome segregation relies on kinetochores that assemble on specialized centromeric chromatin containing a histone H3 variant. In budding yeast, a single centromeric nucleosome containing Cse4 assembles at a sequence-defined 125 bp centromere. Yeast centromeric sequences are poor templates for nucleosome formation in vitro, suggesting the existence of mechanisms that specifically stabilize Cse4 nucleosomes in vivo.
View Article and Find Full Text PDFConnections between the mechanical properties of DNA and biological functions have been speculative due to the lack of methods to measure or predict DNA mechanics at scale. Recently, a proxy for DNA mechanics, cyclizability, was measured by loop-seq and enabled genome-scale investigation of DNA mechanics. Here, we use this dataset to build a computational model predicting bias-corrected intrinsic cyclizability, with near-perfect accuracy, solely based on DNA sequence.
View Article and Find Full Text PDFPreviously, we reconstituted a minimal functional kinetochore from recombinant proteins that was capable of transmitting force from dynamic microtubules to nucleosomes containing the centromere-specific histone variant Cse4 (Hamilton et al. 2020). This work revealed two paths of force transmission through the inner kinetochore: through Mif2 and through the Okp1/Ame1 complex (OA).
View Article and Find Full Text PDFArticle Synopsis
- Accurate mitosis requires chromosomes to achieve "biorientation," where sister chromatids are linked to opposite microtubules through kinetochores.
- Kinetochores initially attach to the sides of microtubules and must subsequently convert these attachments to the plus ends through a trial-and-error method, emphasizing the need for error-correction mechanisms.
- Research shows that kinetochores have a stronger grip on microtubules pulled towards their plus ends, and this sensitivity to microtubule polarity may help stabilize correct connections early in mitosis.
A randomized trial comparing the intraoperative durability of double-gloving with Biogel® surgical gloves to 3 comparators.
Antimicrob Steward Healthc Epidemiol
October 2024
Objective: To determine and compare the intraoperative durability of 4 major surgical glove brands.
Design Setting And Participants: This study is a randomized open-label clinical trial in which surgical gloves from 4 manufacturers are randomized to 5 surgical subspecialty study groups: (1) orthopedic surgery, (2) neurosurgery, (3) colorectal surgery, (4) trauma or acute general surgery, and (5) cardiac and plastic surgeries. The study was divided into 10 periods, with a cross-over design, and was conducted at a tertiary care academic medical center.