[Not Available].

Calciphylaxis is a rare condition characterised by painful necroses due to microvascular calcifications. It primarily affects individuals with end-stage renal disease and affected calcium-phosphate metabolism. This is a case report of a 55-year-old woman with end-stage renal disease who developed a necrotic ulcer at the breast due to calciphylaxis.

Novel amino-Li resin for water-based solid-phase peptide synthesis.

We report the first application of a novel amino-Li resin to water-based solid-phase peptide synthesis (SPPS) applying the Smoc-protecting group approach. We demonstrated that it is a suitable support for the sustainable water-based alternative to a classical SPPS approach. The resin possesses good swelling properties in aqueous milieu, provides significant coupling sites, and may be applicable to the synthesis of difficult sequences and aggregation-prone peptides.

Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination.

Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. The binding constant (K) of a protein-antibody complex is first determined by immobilizing an antibody or DNA-aptamer on an SPR chip.

[Breast reconstruction with a breast-sharing technique].

Many techniques have been described for breast reconstruction after mastectomy, and in many cases a significant reduction of the contralateral breast is needed for symmetry. This is a case report of a single-stage secondary breast reconstruction after mastectomy using the excided tissue from the contralateral reduced breast based on the perforant vessels of the internal thoracic artery. We used this technique in a 62-year-old woman with a hypertrophic contralateral breast, who had undergone mastectomy due to breast cancer.

Sustainable Peptide Synthesis Enabled by a Transient Protecting Group.

The growing interest in synthetic peptides has prompted the development of viable methods for their sustainable production. Currently, large amounts of toxic solvents are required for peptide assembly from protected building blocks, and switching to water as a reaction medium remains a major hurdle in peptide chemistry. We report an aqueous solid-phase peptide synthesis strategy that is based on a water-compatible 2,7-disulfo-9-fluorenylmethoxycarbonyl (Smoc) protecting group.