Publications by authors named "C Sureau"
Individuals chronically infected with hepatitis B virus (HBV) and hepatitis Delta virus (HDV) present an increased risk of developing cirrhosis and hepatocellular carcinoma in comparison to HBV mono-infected individuals. Although HDV only replicates in individuals coinfected or superinfected with HBV, there is currently no in vitro model that can stably express both viruses simultaneously, mimicking the chronic infections seen in HBV/HDV patients. Here, we present the HepG2BD cell line as a novel in vitro culture system for long-term replication of HBV and HDV.
View Article and Find Full Text PDFSelective inhibition of hepatitis B virus internalization by oxysterol derivatives.
Biochem Biophys Res Commun
October 2023
Given that the current approved anti-hepatitis B virus (HBV) drugs suppress virus replication and improve hepatitis but cannot eliminate HBV from infected patients, new anti-HBV agents with different mode of action are urgently needed. In this study, we identified a semi-synthetic oxysterol, Oxy185, that can prevent HBV infection in a HepG2-based cell line and primary human hepatocytes. Mechanistically, Oxy185 inhibited the internalization of HBV into cells without affecting virus attachment or replication.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to evaluate the connection between the concentration of anti-HBs antibodies and their ability to neutralize the hepatitis B virus, revealing that higher antibody levels do not always correspond to better neutralization effectiveness.
- It involved comparing IgG antibodies from individuals who received different types of hepatitis B vaccines or recovered from infection and testing their neutralization activity in vitro.
- The findings suggest that measuring anti-HBs levels alone is inadequate for assessing neutralization, recommending the inclusion of neutralization assays in quality control and highlighting the importance of matching vaccine types with circulating HBV strains.
Current anti-hepatitis B virus (HBV) drugs are suppressive but not curative for HBV infection, so there is considerable demand for the development of new anti-HBV agents. In this study, we found that fungus-derived exophillic acid inhibits HBV infection with a 50% maximal inhibitory concentration (IC50) of 1.1 µM and a 50% cytotoxic concentration (CC50) of >30 µM in primary human hepatocytes.
View Article and Find Full Text PDFIntracellular transport via microtubule-based dynein and kinesin family motors plays a key role in viral reproduction and transmission. We show here that Kinesin Family Member 4 (KIF4) plays an important role in HBV/HDV infection. We intended to explore host factors impacting the HBV life cycle that can be therapeutically addressed using siRNA library transfection and HBV/NLuc (HBV/NL) reporter virus infection in HepG2-hNTCP cells.
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