Isolation and characterisation of an antifungal antibiotic (GR135402) with protein synthesis inhibition.

A novel antifungal antibiotic GR135402 has been isolated from a fermentation broth of Graphium putredinis which inhibited protein synthesis in Candida albicans but not rabbit reticulocytes. The spectrum of activity included C. albicans and Cryptococcus neoformans but not some other Candida species or Aspergillus species.

The squalestatins, novel inhibitors of squalene synthase produced by a species of Phoma. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activity.

During the screening of fungi for inhibitors of squalene synthase, Phoma sp. C2932 was found to produce three structurally related novel inhibitors. These compounds, designated the squalestatins, exhibited potent activity against both mammalian (rat liver) and fungal (Candida albicans) squalene synthase.

Determination of ionisation constants of radiopharmaceuticals in mixed solvents by HPLC.

The uptake of some ionogenic radiopharmaceuticals may be explained on the basis of a pH shift theory. An HPLC method for pKa measurement has been developed for radiotracers. It has been tested with several amines and used to estimate the pKsa (pKa in the mixed solvent 30% acetonitrile-water) of some 99mTc-PnAO complexes by observing the change in capacity factor k' with change in mobile phase pH.