Publications by authors named "C Stolfi"
Gut homeostasis depends on maintaining a fine equilibrium between the intestinal epithelial barrier, the microbiota, and the host's immune system [...
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- In patients with inflammatory bowel disease (IBD), interleukin-34 (IL-34) activates harmful signaling pathways, but the triggers for its production are not well-understood.
- The study found that bromodomain-containing 4 (BRD4) is over-expressed in IBD and may enhance IL-34 production, with both proteins showing increased levels and co-localization in inflammatory cells.
- Experiments demonstrated that inhibiting BRD4 led to reduced IL-34 expression, suggesting that BRD4 plays a key role in regulating IL-34 and contributing to inflammation in IBD.
Interactions between colorectal cancer (CRC) cells and the noncancerous cells in the tumor microenvironment (TME) induce mechanisms for the escape of tumor cells from immune attack. Hepcidin, a peptide that controls immune cell functions, is overproduced by CRC cells. This study aimed to evaluate whether hepcidin acts as a regulator of anti-tumor immunity in CRC.
View Article and Find Full Text PDFIn the colorectal cancer (CRC) niche, the transcription factors signal transducer and activator of transcription 3 (STAT3) and nuclear factor-κB (NF-κB) are hyperactivated in both malignant cells and tumor-infiltrating leukocytes (TILs) and cooperate to maintain cancer cell proliferation/survival and drive protumor inflammation. Through drug repositioning studies, the anthelmintic drug rafoxanide has recently emerged as a potent and selective antitumor molecule for different types of cancer, including CRC. Here, we investigate whether rafoxanide could negatively modulate STAT3/NF-κB and inflammation-associated CRC.
View Article and Find Full Text PDFBackground And Aim: Bromodomain-containing protein 4 (BRD4), one of the components of the bromodomain and extraterminal domain (BET) family, is a transcriptional and epigenetic regulator of cellular proliferation and cytokine production. In this study, we assessed whether BRD4 regulates the cytokine response in inflammatory bowel diseases (IBD).
Materials And Methods: BRD4 expression was analyzed in intestinal mucosal samples of patients with ulcerative colitis (UC), patients with Crohn's disease (CD), normal controls (CTRs), and mice with chemically-induced colitis by real-time PCR, Western blotting, and confocal microscopy.