Spore inactivation on solid surfaces by vaporized hydrogen peroxide-Influence of carrier material surface properties.

The aim of this study was to investigate the influence of surface hydrophobicity and roughness of carrier materials on the inactivation of bacterial spores with gaseous hydrogen peroxide whereas condensate formation is prevented. Spores of Bacillus subtilis and Bacillus atrophaeus were applied either as single spore culture or as a mixed spore population to simulate natural contamination with microorganisms of different characteristics. Inactivation with gaseous hydrogen peroxide was carried out at 5200 ppm hydrogen peroxide without condensate formation.

Influence of spore and carrier material surface hydrophobicity on decontamination efficacy with condensing hydrogen peroxide vapour.

Aims: To investigate the influence of surface hydrophobicity of carrier material (CM) and bacterial spores of Bacillus subtilis SA 22 and Bacillus atrophaeus (DSM 675) on spore inactivation with condensing hydrogen peroxide (H O ) gas.

Methods And Results: Surface hydrophobicity of bacterial spores and CM was determined by means of contact angle measurement. Spores of B.