Publications by authors named "C Schilde"
Article Synopsis
- The research explores how adhesion forces among submicron particles affect processes like agglomeration, focusing on factors like particle size and surface roughness.
- Experiments were conducted using colloidal probe atomic force microscopy (cp-AFM) to measure adhesive forces in silica and polystyrene, while surface energies were assessed through the capillary rise method.
- Findings reveal a relationship between measured adhesion forces and surface energy using various models, identifying substrate and particle roughness, as well as material behavior, as key factors impacting adhesion.
Background: Cyclic di-guanylate (c-di-GMP), synthesized by diguanylate cyclase, is a major second messenger in prokaryotes, where it triggers biofilm formation. The dictyostelid social amoebas acquired diguanylate cyclase (dgcA) by horizontal gene transfer. Dictyostelium discoideum (Ddis) in taxon group 4 uses c-di-GMP as a secreted signal to induce differentiation of stalk cells, the ancestral somatic cell type that supports the propagating spores.
View Article and Find Full Text PDFIn biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress to achieve high product concentrations. Macroparticles were added to shake flask cultures of the filamentous actinomycete to find these optimal cultivation conditions.
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- * A study analyzing GPCRs in Amoebozoan genomes revealed that most GPCRs are ancient and involved in basic cell functions and environmental sensing, with specific families showing varied presence and roles in dictyostelids.
- * Phylogenetic analysis indicates early divergence of certain GPCR families in Amoebozoa, while some GPCRs have been adapted for signaling and multicellular development in dictyostelids, marking a significant evolutionary transition.
Protein kinases are major regulators of cellular processes, but the roles of most kinases remain unresolved. Dictyostelid social amoebas have been useful in identifying functions for 30% of its kinases in cell migration, cytokinesis, vesicle trafficking, gene regulation and other processes but their upstream regulators and downstream effectors are mostly unknown. Comparative genomics can assist to distinguish between genes involved in deeply conserved core processes and those involved in species-specific innovations, while co-expression of genes as evident from comparative transcriptomics can provide cues to the protein complement of regulatory networks.
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