Physiological responses of the M sainfoin (Onobrychis viciifolia Scop) plants to gamma radiation.

Effects of gamma radiation on physiological responses of the M sainfoin plants were investigated. Seeds of sainfoin ecotype 'Koçaş' were exposed to 0, 400, 500 and 600Gy from a Co source at a dose rate of 0.483 kGyh.

Efficient callus induction and plant regeneration from mature embryo culture of winter wheat (Triticum aestivum L.) genotypes.

Immature and mature embryos of 12 common winter wheat (Triticum aestivum) genotypes were cultured in vitro to develop an efficient method of callus formation and plant regeneration from mature embryo culture, and to compare the responses of both embryo cultures. Fifteen days after anthesis, immature embryos were aseptically dissected from seeds and placed with the scutellum upwards on a solid agar medium containing the inorganic components of Murashige and Skoog (MS) and 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). Mature embryos were moved slightly in the imbibed seeds.

Prolific shoot regeneration from immature embryo explants of sainfoin (Onobrychis viciifolia Scop.).

Immature cotyledons and embryo axes of sainfoin were cultured on Murashige and Skoog (MS) media supplemented with various concentrations of 6-benzylaminopurine (BAP) and a-naphthaleneacetic acid (NAA) to induce adventitious shoot regeneration. The highest frequency of shoot regeneration occurred following an initial callus growth on a MS medium containing 0.5 mg/l BAP and 2 mg/l NAA.