Publications by authors named "C Samakovlis"
Targeted spatial transcriptomic methods capture the topology of cell types and states in tissues at single-cell and subcellular resolution by measuring the expression of a predefined set of genes. The selection of an optimal set of probed genes is crucial for capturing the spatial signals present in a tissue. This requires selecting the most informative, yet minimal, set of genes to profile (gene set selection) for which it is possible to build probes (probe design).
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- The study focuses on the lung's development during the first trimester of human pregnancy, emphasizing its importance for life on land.
- It utilizes advanced imaging and analysis techniques to map the protein-level organization of lung cells over various stages of gestation, examining more than 2 million cells.
- The findings offer a detailed resource on the types of cells in the developing lung as well as their growth and spatial patterns, serving as a foundation for future research on respiratory health.
Article Synopsis
- Fibrosis, especially idiopathic pulmonary fibrosis (IPF), is linked to abnormal healing processes in the lungs that can lead to organ failure, with no current cure.
- The study investigates activated myofibroblasts (aMYFs), their different subtypes, and their roles in lung repair and damage using genetic and transcriptomic analysis in mice, as well as human data.
- Findings reveal that aMYFs can be categorized into four distinct groups, with a specific subset linked to both the progression and resolution of fibrosis, suggesting new potential treatment targets for managing IPF.
Background: The precise origin of newly formed ACTA2+ (alpha smooth muscle actin-positive) cells appearing in nonmuscularized vessels in the context of pulmonary hypertension is still debatable although it is believed that they predominantly derive from preexisting vascular smooth muscle cells (VSMCs).
Methods: mice were used to lineage trace GLI1+ (glioma-associated oncogene homolog 1-positive) cells in the context of pulmonary hypertension using 2 independent models of vascular remodeling and reverse remodeling: hypoxia and cigarette smoke exposure. Hemodynamic measurements, right ventricular hypertrophy assessment, flow cytometry, and histological analysis of thick lung sections followed by state-of-the-art 3-dimensional reconstruction and quantification using Imaris software were used to investigate the contribution of GLI1+ cells to neomuscularization of the pulmonary vasculature.
Background: Fibroblast-to-myofibroblast conversion is a major driver of tissue remodelling in organ fibrosis. Distinct lineages of fibroblasts support homeostatic tissue niche functions, yet their specific activation states and phenotypic trajectories during injury and repair have remained unclear.
Methods: We combined spatial transcriptomics, multiplexed immunostainings, longitudinal single-cell RNA-sequencing and genetic lineage tracing to study fibroblast fates during mouse lung regeneration.